First Aid General Bacteriology Flashcards
What media is used for isolation of lactose-fermenting bugs?
MacConkey agar

Fermentation produces acid, causing colonies to turn pink.
Chocolate agar is used for isolation of what bug?
H. influenzae

What media is used for isolation of Legionella?
Charcoal yeast extract agar buffered with cysteine and iron

Tellurite agar and Löffler medium are used to isolate what bug?
C. diphtheriae
Löwenstein-Jensen agar is used for isolation of what bug?
M. tuberculosis

Eosin–methylene blue (EMB) agar is used for isolation of what bug?
E. coli
Colonies with green metallic sheen

What are the media contents of Bordet-Gengou agar?
potato
(Bordet-Gengou is red, Regan-Lowe is black)

Charcoal yeast extract agar buffered with cysteine and iron is used for isolation of what bug?
Legionella

Bordet-Gengou agar (Bordet for Bordetella) and Regan-Lowe medium are used for isolation of what bug?
B. pertussis
(Bordet-Gengou is red, Regan-Lowe is black)

What media is used for isolation of N. gonorrhoeae and N. meningitidis?
Thayer-Martin agar

What media is used for isolation of B. pertussis?
- Bordet-Gengou agar (Bordet for Bordetella) (red)
- Regan-Lowe medium (black)

What media is used for isolation of M. tuberculosis?
Löwenstein-Jensen agar

Eaton agar is used for isolation of what bug?
M. pneumoniae
What media is used for isolation of E. coli?
Eosin–methylene blue (EMB) agar
Colonies with green metallic sheen

Thayer-Martin agar is used for isolation of what bugs?
N. gonorrhoeae, N. meningitidis

What media is used for isolation of C. diphtheriae?
Tellurite agar, Löffler medium
Sabouraud agar is used for isolation of what bugs?
Fungi
“Sab’s a fun guy!”

What media is used for isolation of H. influenzae?
Chocolate agar

What are the media contents of Regan-Lowe medium?
Charcoal, blood, and antibiotic
(Bordet-Gengou is red, Regan-Lowe is black)

Factors V (NAD+ and X (hematin) are the media contents of what bacterial growth media?
chocolate agar

What media is used for isolation of M. pneumoniae?
Eaton agar
What are the media contents of Thayer-Martin agar?
- Vancomycin (inhibits gram-positive organisms),
- Trimethoprim,
- Colistin (inhibits gram-negative organisms except Neisseria), and
- Nystatin (inhibits fungi)
Very Typically Cultures Neisseria

MacConkey agar is used for isolation of what bugs?
Lactose-fermenting enterics
Fermentation produces acid, causing colonies to turn pink.
