First Aid General Bacteriology Flashcards

1
Q

What media is used for isolation of lactose-fermenting bugs?

A

MacConkey agar

Fermentation produces acid, causing colonies to turn pink.

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2
Q

Chocolate agar is used for isolation of what bug?

A

H. influenzae

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3
Q

What media is used for isolation of Legionella?

A

Charcoal yeast extract agar buffered with cysteine and iron

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4
Q

Tellurite agar and Löffler medium are used to isolate what bug?

A

C. diphtheriae

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5
Q

Löwenstein-Jensen agar is used for isolation of what bug?

A

M. tuberculosis

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6
Q

Eosin–methylene blue (EMB) agar is used for isolation of what bug?

A

E. coli

Colonies with green metallic sheen

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7
Q

What are the media contents of Bordet-Gengou agar?

A

potato

(Bordet-Gengou is red, Regan-Lowe is black)

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8
Q

Charcoal yeast extract agar buffered with cysteine and iron is used for isolation of what bug?

A

Legionella

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9
Q

Bordet-Gengou agar (Bordet for Bordetella) and Regan-Lowe medium are used for isolation of what bug?

A

B. pertussis

(Bordet-Gengou is red, Regan-Lowe is black)

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10
Q

What media is used for isolation of N. gonorrhoeae and N. meningitidis?

A

Thayer-Martin agar

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11
Q

What media is used for isolation of B. pertussis?

A
  • Bordet-Gengou agar (Bordet for Bordetella) (red)
  • Regan-Lowe medium (black)
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12
Q

What media is used for isolation of M. tuberculosis?

A

Löwenstein-Jensen agar

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13
Q

Eaton agar is used for isolation of what bug?

A

M. pneumoniae

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14
Q

What media is used for isolation of E. coli?

A

Eosin–methylene blue (EMB) agar

Colonies with green metallic sheen

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15
Q

Thayer-Martin agar is used for isolation of what bugs?

A

N. gonorrhoeae, N. meningitidis

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16
Q

What media is used for isolation of C. diphtheriae?

A

Tellurite agar, Löffler medium

17
Q

Sabouraud agar is used for isolation of what bugs?

A

Fungi

Sab’s a fun guy!”

18
Q

What media is used for isolation of H. influenzae?

A

Chocolate agar

19
Q

What are the media contents of Regan-Lowe medium?

A

Charcoal, blood, and antibiotic

(Bordet-Gengou is red, Regan-Lowe is black)

20
Q

Factors V (NAD+ and X (hematin) are the media contents of what bacterial growth media?

A

chocolate agar

21
Q

What media is used for isolation of M. pneumoniae?

A

Eaton agar

22
Q

What are the media contents of Thayer-Martin agar?

A
  • Vancomycin (inhibits gram-positive organisms),
  • Trimethoprim,
  • Colistin (inhibits gram-negative organisms except Neisseria), and
  • Nystatin (inhibits fungi)

Very Typically Cultures Neisseria

23
Q

MacConkey agar is used for isolation of what bugs?

A

Lactose-fermenting enterics
Fermentation produces acid, causing colonies to turn pink.