finals lesson 4 Flashcards
Mostsensitive indicator of postmortem
change
Mitochondriaand endoplasmic reticulum
➢ Usesaldehyde
○ Ex. glutaraldehyde
○ Stabilizesproteins
○ Effectiveat 1.5% to
primary fixation
➢ Usesosmium tetroxide
○ Retains lipids
○ Effectiveat 1% or 2%
○ Mustbe handled well because the sediments that can be form with this, can lead to blindness
secondary fixation
Osmiumtetroxide is used at what temperature
room temperature
what is the duration in 0.5-1 mm3 in 2.5% glutaraldehyde
2-6hrs
duration in 1% osmiumtetroxide
60-90 minutes
buffer of choice in fixation
phosphate buffer
Fixatives buffered at what pH
pH 7.2-7.6
Embedding material that is not miscible
with water
epoxy resin
Avoidacetone if tissue will be stained with ___________
uranyl acetate
Embeddingmedium of choice
epoxy resin
Cutonly with glass knives
semi-thin sections
➢ Needed if larger viewing areas are required
➢ Plasticsfilms from collodion, formvar, butvar
support films
➢ Silverto straw colored sections
➢ Collectsections with a grid immerse in the floatation fluid
ultra thin sectioning
Colorproduced in staining
shades of gray
Double stranded DNA, helix pattern is developed by
➢ 1953, JamesWatson & Francis Crick
-Developed blotting technique for DNA
-Southern blot
Edwin southern, 1975
Developed of polymerase chain reaction
1985, Kary Mullis
Theflow of information from DNA to RNA to proteins
central dogma
The process by which a
molecule of DNA is duplicated
DNA replication
The process of copying information from DNA into new strands of messenger RNA (mRNA
transcription
