Finals Flashcards

1
Q

When are blood cultures ordered

A

when there is Fever of Unknown Origin (FUO)

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2
Q

LIst the 3 aims of blood culture

A
  • Confirm the infectious etiology
  • Identify the etiological agent
  • Guide the antimicrobial therapy
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3
Q

a positive blood culture means what

A

there is bacteria in blood

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4
Q

a systemic infection is known as

A

sepsis

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5
Q

blood cultures should be requested when

A

bloodstream infection or sepsis is suspected

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6
Q

clinical symptoms in a patient that can lead to suspicious of bloodstream infection

A
  • undetermined fever or chills
  • severed local infections
  • abnormally raised heart rate
  • low or raised body temperature
  • raised respiratory rate
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7
Q

yellow top

A

pediatric aerobic (1-3mLgree)

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8
Q

green top

A

adult aerobic (5-10mL)

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9
Q

orange top

A

anaerobic (5-10mL)

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10
Q

black top

A

mycobacteria (5-10mL)

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11
Q

silver

A

mycoplasma (3-5mL)

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12
Q

if using a winged blood collection set, which bottle should be filled

A

aerobic bottle

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13
Q

if using needle and syringe which bottle should be filled first

A

anaerobic bottle

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14
Q

under-filling can cause

A

false negative results

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15
Q

over-filling can cause

A

inaccurate results

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16
Q

most important part of blood culture

A

antiseptics

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17
Q

alternative collection procedure for small amounts of blood

A

capillary puncture

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18
Q

it is the usual collection procedure for infants and children under 2 years old

A

capillary puncture

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19
Q

used for bleeding times, ancillary blood glucose testing, and obese patients

A

capillary puncture

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20
Q

mixture of arterial blood, venous blood and tissue fluid

A

capillary blood

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21
Q

bladed instrument that either puncture or makes an incision in the skin to obtain capillary blood specimens for testing

A

Lancet

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22
Q

use to disinfect the area before incision

A

alcohol pads

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23
Q

plain tubes with no anticoagulant

A

blue capillary tubes

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24
Q

heparinized tubes

A

red capillary tubes

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25
use to seal capillary tubes
sealing clay
26
small plastic tubes used to collect tiny amounts of blood obtained from capillary punctures
microtainer tubes
27
for whole blood hematology and lead determinations
K2EDTA
28
for plasma determinations in clinical chemistry
Litium Heparin
29
for plasma determinations in clinical chemistry
Lithium heparin and gel for plasma separation
30
for glucose determinations
NaFI/Na2 EDTA
31
for serum determinations in clinical chemistry
clot activator with gel for serum seperation
32
for serum determinations in clinical chemistry
no additive
33
order of draw for capillary puncture
1. capillary blood gases 2. blood smear 3. EDTA tubes 4. other anticoagulant tubes 5. serum tubes
34
capillary puncture site for babies
planter surface of heel
35
depth of capillary puncture for babies
2mm
36
cause of hemolysis in capillary puncture for babies
1. alcohol not completely dried 2. finger or heel squeezed to vigorously 3. blood was scraped off 4. newborns have increased RBC fragility
37
defined as the time it takes for a standard wound to stop bleeding
bleeding time
38
duke's BT
earlobe/fingertip
39
Ivy BT
* volar surface of arm with BP cuff of 40mm Hg * pressure of BP cuff will vary with age
40
Template BT
* modification of Ivy * uses a template or a commercial device
41
causes of Prolonged BT (bleeding time)
* thrombocytopenia * qualitative platelet disorders *blood vessel abnormality * drug therapy
42
a process of spinning down or separating the cells from liquid portion of blood
centrifugation
43
a laboratory device that is used for the separation of fluids, gas or liquid, based on density.
centrifuge
44
centrifugal force is expressed as
gravity
45
of the centrifuge vibrates and makes noises, this means that
the centrifuge is unbalanced
46
centrifuge must be calibrated with a
tachometer
47
centrifuge that is * commonly used in labs * can take up to 100 tubes * slow speed (up to 4000 rpm)
small benchtop
48
centrifuge that is * takes tubes of small volume (2mL) * commonly used in biochemistry, molecular biology. and biological labs * can generate forces up to ~15,000 x g
microcentrifuge
49
centrifuge that is * normally refrigerated * 15,000 - 20,000 rpm * research applications * differentiation separation of nucleus, mitochondrial, protein precipitate, large intact organelle, cellular debris, bulky protein aggregates
high speed centrifuges
50
centrifuge that is * expensive!! * requires special rotors * 65,000 - 120,00 rpm * balance is important
ultracentrifuge
51
centrifuge that is * centrifugal fields of 3000 - 6000 x g * efficient separation of coarse precipitate or whole cells
large-capacity preparative centrifuge
52
used for determination of volume fraction of erythrocytes in blood and also for separation of microblood and solutions
hematocrit centrifuge
53
a procedure of dividing or separating specimens into separate containers patient samples
aliquoting
54
if red cells were left in contact with serum or plasma, glucose will decrease and potassium can increase. This process is called what
glycolysis
55
greatest concerns of all laboratory workers
mixing up patient samples
56
the time take for blood to clot in a person
clotting time
57
advantages of lee and white method
1. more accurate and standard method 2. test can be run with control
58
disadvantage of lee and white method
1. it is also a rough method 2. there can be contamination of syringe or tube
59
normal clotting time for lee and white method
5-10 minutes
60
advantages of capillary tube method
can be performed when venous blood cannot be obtained
61
disadvantages of capillary tube method
1. method is insensitive 2. method is unreliable
62
what significance does high value time for clotting time have?
1. hemophilia disorders 2. congenital deficiencies in clotting factors
63
what significance does low value time for clotting time have?
* disseminated intravascular coagulation (DIC) * thrombotic predisposition
64
normal value for clotting time
2-4 minutes
65
used to determine whether the ulnar artery can provide collateral circulation to the hand after the radial artery puncture
allen test
66
was originally developed by Edgar V. Allen in 1929
allen test
67
if allen test is negative, what should u do
use another site to select
68
portion of a larger whole
Aliquot
69
most laboratory specimens are centrifuged at
750 to 1000g for 15 minutes
70
excessive crying of infant can lead to
elevated leukocyte count