Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Biochemistry Lab > FINAL review > Flashcards

FINAL review Flashcards

1
Q

what is purpose of Lowry assay

A

determine protein level in solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What color is generated in Lowry assay and what absorbance level is it measured

A

blue color; 750nm and 540 nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

the total protein concentration in Lowry assay is exhibited by what

A

color change of sample solution in proportion to protein concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how is absorbance measured

A

using spectrophotometer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Lowry reagent \/ Colin phenol is

A

a strong acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

in bradford assay, the absorbance is at

A

595 nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what amino acids are required for Lowry assay

A

tyrosine and tryptophan

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Lowry assay requires samples containing only

A

10-200 ug/ml of protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Lowry assay generates ____ color; biuret assay results in _____ color

A

blue; purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what two groups are found in an amino acid

A

amino group and carboxylic acid group

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

titration curve is a plot of what

A

pH of weak acid against degree of neutralization of acid by strong base

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what does a zwitterion contain

A

a protonated amino group and deprotonated carboxyl group

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what titrants are used in amino acid titration

A

1M KOH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how is unknown amino acid identified in amino acid titration

A

calculating its molecular mass

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

ninhydrin reaction is what type of reaction

A

redox reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what groups are involved in ninhydrin reaction

A

amino group of sample and ninhydrin reagent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

what is end product formed similar to

A

di-dehydrin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

di-ketohydrin has what color and what is it also known as

A

deep blue pigmentation; ruhemann’s purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

what is ninhydrin reagent used for

A

qualitative and quantitive colorimetric analysis for amino acids and sometimes for peptides and proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

what molecules are used in part A of ninhydrin reaction

A

glucose, serine, and glycine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

what technique is used and what is its purpose in ninhydrin reaction

A

thin layer chromatography; used to separate and identify amino acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

what result is a positive test in ninhydrin reaction

A

purple color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

what is function of protease

A

enzyme that catalyzes proteolysis or breakdown of protein (break down peptide bonds)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

how is protease activity measured

A

colormetric method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

what are protease enzymes involved in

A

cell division, growth, apoptosis, and migration

26
Q

TCA is used in protease activity to do what

A

stop the reaction by precipitating enzymes and unreacted substrates

27
Q

ninhydrin reagents can be used in protease activity for what

A

quantification of TCA soluble peptides

28
Q

what enzymes are used in protease activity

A

trypsin and substrate casein

29
Q

enzyme kinetics is study of

A

rates of enzyme catalyzed chemical reactions

30
Q

trypsin plays role of ___ and casein plays role of _____

A

enzyme; substrate

31
Q

what molecule is used to stop enzyme catalyzed reaction

A

trichloroacetic acid

32
Q

molecular weight of casein is

A

33,300 daltons

33
Q

what is name of v vs [s] plot

A

Michaeles Menten plot

34
Q

velocity is ____ variable and substrate conc. is ___ varible

A

dependent; independent

35
Q

galactose is _____ because its aldehyde group is easily oxidized to carboxylic acid

A

reducing sugar

36
Q

what result does sucrose give in resorcinol test

A

positive result

37
Q

sucrose and trehalose are what type of sugars

A

non-reducing sugars

38
Q

purpose of carbohydrate analysis experiment

A

test characteristics of sugars

39
Q

Benedict’s reagents tests is for

A

reducing sugar

40
Q

what is positive result in beneditcs reagent; Molisch test; resorcinol test; iodine-KI test

A

greenish red color (reducing sugar); red violet color (carbohydrate presence); red for ketone sugar and identifies between aldose and ketone sugar; blue color in presence of amylose/starch

41
Q

what is purpose of DNA plasmids isolation and PCR

A

extract DNA plasmids from cell cultures and measure DNA concentration

42
Q

what are steps for PCR

A

denaturation, annealing and extenstion

43
Q

SDS has what purpose in DNA isolation lab

A

detergent that destabilizes cell wall and cause cell wall to burst and release concents

44
Q

TE contains what

A

Tris-HCl which is a buffer to obtain correct pH

45
Q

EDTA is what

A

chelates ions needed by DNAse, inactivate DNAses and keep DNA from degrading

46
Q

another name for DNA fragment is

A

amplicon

47
Q

DNA absorbs light at

A

260 nm and protein at 280nm

48
Q

Agarose gel electrophoresis is good at separating fragments ranging from

A

100 bp to 25kb

49
Q

purpose of DNA agarose gel lab

A

check PCR product and DNA size

50
Q

what is DNA agarose gel made of

A

0.5 g agarose, TBE buffer, and ethidium bromide

51
Q

what is purpose of using UV light in DNA agarose gel

A

visualize DNA

52
Q

ethidium bromide bonded to DNA will glow what color at what wavelength

A

orange; 302

53
Q

comparing mobility of DNA of known size to mobility of

A

your PCR fragment

54
Q

purpose of gel filtration chromatography is

A

purification of biological macromolecules

55
Q

gel filtration chromatography can separate protein of

A

different masses

56
Q

how is molecular mass of unknown protein found

A

by comparing to elution volumes of molcular mass standards

57
Q

how is hydrodynamic radius determined

A

by fluidity instruments …

58
Q

AGE stands for

A

polyacrylamide gel electrophoresis

59
Q

the unique migration rate of protein in SDS PAGE is found by

A

size of protein

60
Q

SDS is considered what

A

an anionic deteregent

61
Q

large negative charge of SDS can

A

impart once bounded to protein

62
Q

what is SDS PAGE used for

A

separate molecules of DNA RNA or protein on basis of size or electric hcarge

Biochemistry Lab (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions