Final Exam - Quizzes

Question 1

Quiz 5

Select one: Relaxed plasmids are _____.

a) The ones that are easily isolated
b) The ones that have loose structures
c) The ones that have a copy number > 20 per cell
d) The ones that are easily digested by endonuclease

Answer 1

c) The ones that have a copy number > 20 per cell

Question 2

Quiz 5

Select all that apply: You want to transform two plasmids into an E. coli strain. Which of the following combinations are feasible?

a) An IncQ plasmid and an IncQ plasmid
b) An IncF plasmid and an IncM plasmid
c) An IncP plasmid and an IncQ plasmid
d) An IncP plasmid and an IncP plasmid

Answer 2

b) An IncF plasmid and an IncM plasmid

c) An IncP plasmid and an IncQ plasmid

Question 3

Quiz 5

Select one: Which of the following process involves the transfer of naked fragments of DNA between bacteria?

a) Conjugation
b) Transformation
c) Transfection
d) Horizontal gene transfer
e) Transduction

Answer 3

b) Transformation

Question 4

Quiz 5

Select one: Conjugation between an F+ bacterium and an F- bacterium will generate _____.

a) one Hfr bacterium and one F- bacterium
b) one F+ bacterium and one F- bacterium
c) two F+ bacteria
d) two F- bacteria
e) one Hfr bacterium bacterium and one F- bacterium

Answer 4

c) two F+ bacteria

Question 5

Quiz 5

Select one: Plasmids carry genetic information coding for enzymes that are associated with all of the following except _____.

a) sex pilus
b) normal cellular activity
c) toluene depredation
d) antibiotic response

Answer 5

b) normal cellular activity

Question 6

Quiz 5

Select one: What information can be determined from the interrupted mating of bacteria?

a) The sequence of the chromosome
b) Chromosomal locations of bacterial genes
c) Bacterial recombining
d) The size of the chromosome

Answer 6

b) Chromosomal locations of bacterial genes

Question 7

Quiz 5

Select one: Which one of the following processes involves a sex plius?

a) Integration of a temperate bacteriophage
b) Conjugation resulting in transfer of an R ( resistance factor )
c) Transposon of a mobile genetic element
d) Transduction of a chromosomal gene

Answer 7

b) Conjugation resulting in transfer of an R ( resistance factor )

Question 8

Quiz 5

Select one: Which bacterial gene transfer process is most sensitive to extracellular nucleases?

a) Vertical gene transfer
b) Transduction
c) Horizontal gene transfer
d) Transformation
e) Conjugation
f) Homologous recombination

Answer 8

d) Transformation

Question 9

Quiz 5

Select one: Which of the following events is MOST likely to be due to bacterial conjugation?

a) A Pseudomonas areginosa strain produces B-lactamase encoded by a plasmid similar to a plasmid of another gram-negative organism
b) A gene encoding resistance to gentamicin in the E. coli chromosome appears in the genome of a bacteriophage that has infected E. coli
c) A Corynebacterium diphtheria strain produces a toxin encoded by a prophage
d) An encapsulated strain of Streptococcus pneumonia acquires the gene for capsule formation from an extract of DNA from another encapsulated strain.

Answer 9

? check?

a) A Pseudomonas areginosa strain produces B-lactamase encoded by a plasmid similar to a plasmid of another gram-negative organism

Question 10

Quiz 6

Fill in the blank: Lysogenic phages are also called ____ phages

Answer 10

temperate

Question 11

Quiz 6

Select all that apply: Lambda phage will ____.

a) not result in any plaques on a lawn of E. coli
b) generate clear plaques on a lawn of E. coli
c) generate cloudy plaques on a lawn of E. coli
d) not be able to infect lamda lysogens
e) infect lamda lysogens and generate clear plaques

Answer 11

c) generate cloudy plaques on a lawn of E. coli

d) not be able to infect lamda lysogens

Question 12

Quiz 6

Select all that apply: The pLysE is used in the pET system ____.

a) when a toxin protein is to be overproduced
b) to enhance the expression of the target gene
c) to inhibit leaky expression of the T7 RNAP
d) to help lyse the host cell

Answer 12

a) when a toxin protein is to be overproduced

c) to inhibit leaky expression of the T7 RNAP

Question 13

Quiz 6

Select one: In the lambda lysogen, ____ is the only lambda protein being produced.

a) attP
b) Cro
c) O protein
d) CI
e) Int
f) N

Answer 13

d) CI

Question 14

Quiz 6

Select one: Specialized transduction can be distinguished from generalized transduction by the fact that ____.

a) lysogeny is involved in the former but usually not the latter
b) transducing particles are involved in the former but usually not the latter
c) a selective medium is required to demonstrate the former but usually not the latter
d) homologous recombination is involved in the former but usually not the latter

Answer 14

a) lysogeny is involved in the former but usually not the latter

Question 15

Quiz 6

Select all that apply: Induction of the lambda lytic cycle requires ____.

a) DNA Pol II and Pol V
b) RecA
c) LexA
d) Xis
e) Int

Answer 15

b) RecA
d) Xis
e) Int

Question 16

Quiz 6

Select all that apply: Homologous recombination could occur ____.

a) during conjugation
b) during transformation
c) during transposition
d) during the formation of prophages
e) during transduction

Answer 16

? check ?

a) during conjugation
b) during transformation
d) during the formation of prophages

Question 17

Quiz 8

Select one: Why does nonhomologous recombination occur less frequently than homologous recombination?

a) Nonhomologous is not involved in regular cellular activities
b) Nonhomologous typically involves recognizing highly specific sites
c) Homologous does not require any enzymes
d) Homologous is the preferred mechanism

Answer 17

b) Nonhomologous typically involves recognizing highly specific sites

Question 18

Quiz 8

In the Lederberg and Tatum experiment, why did they have both strain A and strain B lack the ability to produce 2 or 3 biosynthetic compounds respectively?

a) They needed to involve the regulatory pathway
b) To ensure that phototrophic colonies did not just have a reversion of the mutation
c) They wanted to make it more difficult for the bacteria to mate
d) They were eating specific biosynthetic effects

Answer 18

b) To ensure that phototrophic colonies did not just have a reversion of the mutation

Question 19

Quiz 8

Which of the following statements regarding the TA system is/are correct?

a) Some antitoxins act like endonucleases to degrade toxin mRNA directly
b) The toxin of the MazEF system prevents cells division by inhibiting the cytoskeletal proteins
c) Some TA systems can protect the bacteria population from orange infection
d) The Edin genes are repressed by LexA. Depression of these genes will lead to cell death
e) The antitoxin can be a protein

Answer 19

a) Some antitoxins act like endonucleases to degrade toxin mRNA directly
c) Some TA systems can protect the bacteria population from orange infection
d) The Edin genes are repressed by LexA. Depression of these genes will lead to cell death
e) The antitoxin can be a protein

Question 20

Quiz 8

If a mutated lambda phase page infects a lawn of E. coli and generates clear plaques, which of the following could be the possible mutations of the lambda phage?

a) A constitutive bro expression
b) A cIII::Tn10
c) A deletion in Pre
d) An over-expression of CII
e) A frameshift occurred near the amnio - terminus of the N protein

Answer 20

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