Final Exam Bench notes

Question 1

Stools Bench:

what enteric pathogens are oxidase positive on SBAP

Answer 1

Aeromonas
Plesiomonas
Vibrio

Question 2

Stools Bench:

what is SMAC plate used for and how does this organism look macroscopically?

Answer 2

E.coli O157

clear colorless colonies

Question 3

Stools Bench:
what pathogens grow on MAC plate?
how do they look?

Answer 3

salmonella 
shigella 
plesiomonas 
aeromonas 
Yersinia
(clear colorless)

Question 4

Stools Bench:

what Pathogens grow on XLD and how do they look?

Answer 4

Shigella- red

Salmonellla-red with black center

Question 5

Stools Bench:

what Media is used to isolate Campy?

Answer 5

CVA

Question 6

Stools Bench:

What media is used to isolate and culture Vibrio? How does Vibrio look macroscopically on this media?

Answer 6

TCBS/vibro

Question 7

Stools Bench:

Why do we incubate the MAC plate for 3 days?

Answer 7

Yersinia

Question 8

P/0 Bench:

what are the stains used at this bench and what are they used for?

Answer 8

• Trichrome stain
• iodine
• modified acid fast-isospora/cyclospora

Question 9

P/0 Bench:

what pathogen is ID from corneal scrapings or contact lenses?

Answer 9

acanthomebia

-blinking on agar

Question 10

P/0 Bench:

what 2 organisms are ID from a KIt test?

Answer 10

Crypto/Giardia

Question 11

Wounds:

• pleomorphic gram + rod
• pinpoint colonies

Answer 11

cory

Question 12

Wounds:
Pathogen of vulva
Gram + cocci

Answer 12

Group A strep

Strep. Pyogenes

Question 13

meningitis in teens to mid twenties

Answer 13

Neisseria menigitidis

Question 14

causes meningitis in young kids

Answer 14

Haemophilus influenzae

Question 15

causes meningitis in old people

Answer 15

Strep pneumo

Question 16

causes meningitis in neonates

Answer 16

Group B Strep

Question 17

what are the Two Drug associated with the D test used for Pseudo?

Answer 17

Clindamycin and erythromycin

Question 18

Anaerobes:
what is/ are the gram neg anaerobes?
**which is the only negative diplococci

Answer 18

Veillonella parvula***
paraphyromonas spp.
prevotella melaninogenica
bacteriodes fragilis
fusobacterium spp.

Question 19

Anaerobes:
what anaerobe is bile Esculine +
Resistant to Penicillin, Kenamycin, Vanco and Colliston?

Answer 19

Bacteriodes fragilis

Question 20

Anaerobes:

what organism is black pigmented on Lacked Blood agar?

Answer 20

prevetella melaninogenica

Question 21

Anaerobes:

what gram neg cannot metabolize carbs?

Answer 21

Paraphyromonas spp.

Question 22

what common pathogens are Oxidase Positive?

Answer 22

Ps. aeruginosa
Aeromonas spp.
Vibrio

Question 23

Stools Bench:

How does E.coli look on TSI?

Answer 23

yellow slant/ yellow butt ,gas+

Question 24

Stools Bench:

How does Shigella sonnei look on TSI?

Answer 24

red/yellow no gas

Question 25

how does Salmonella enterica look on TSI?

Answer 25

red/yellow gas + black butt

Question 26

how does PSeudo look on TSI?

Answer 26

red/red

Question 27

Mycology: | what organisms is TBO stain used for?

Answer 27

-encapsulated organisms: | specifically Pneumocystis, Cryptococcus spp., Candida spp. ,Blasto

Question 28

Mycology: | what characteristic sets Cryptococcus neoformans apart from other Crypto?

Answer 28

- pos bird seed agar test

Question 29

Mycology: | what organisms are urease pos?

Answer 29

H. Pylori and Cryptococcus spp.

Question 30

Mycology: | what candida spp. assilates trehalose?

Answer 30

candida glabrata?

Question 31

Mycology: | what dimorphic fungus is tuberculated at 30?

Answer 31

Histo

Question 32

Mycology: | what dimorphic fungus looks like a lollipop at 30?

Answer 32

Blasto

Question 33

Mycology: | what dimorphic fungus has a broad base mother cell that is 20 um at 37?

Answer 33

Blasto

Question 34

Mycology: | what dimorphic fungus is 70um spherale at 37?

Answer 34

cocci

Question 35

Mycology: | what dimorphic fungus is barrel shapped at 30?

Answer 35

coccidiodes immitis

Question 36

Mycology: what class of fungus DO NOT GRIND?? -which all have this feature? -what drug inhibits these?

Answer 36

- Zygomyces - non-septate - cycohexamide

Question 37

``` Mycology: This class of fungus have a birds on a fence appearance ```

Answer 37

Dermatophytes

Question 38

Mycology: | what fungus do we use olive oil disks on plates to grow?

Answer 38

malizezia furfur?

Question 39

Mycology: | what dimorphic fungus is cigar chaped yeast?

Answer 39

sporothrix

Question 40

Mycology: | what dimorphic fungus has a yeast form that is red pigmented is promenint in S.E Asia and common in HIV patients?

Answer 40

Pen. marnefi

Question 41

Mycology: | why does CSF specimens only get BHI?

Answer 41

cryptococcus

Question 42

Mycology: | what plate is best to do lact blue off of?

Answer 42

PDA

Question 43

Mycology: | what lytic agent lysis RBCs when using the DuPont isolator?

Answer 43

supponin

Question 44

AST: | what are the antibiotics that pseudo is suscept. too?

Answer 44

aminoglycosides: gentamycin, ampicylin,

Question 45

what media is fastidious organisms plated too for AST and what is used to make the McFarland? instead of ?

Answer 45

- Blood, BHI | - Mueller hinton, saline

Question 46

AST: | What drugs are used for E.coli?

Answer 46

Tempsola and ampcylin

Question 47

Anaerobes: ric gram neg pointed ends

Answer 47

Fuso

Question 48

what organisms are indole +

Answer 48

Proteus vulgaris. Klebsiella oxytoca E.coli