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Clinicals Diag Micro > Final Exam Bench notes > Flashcards

Final Exam Bench notes Flashcards

1
Q

Stools Bench:

what enteric pathogens are oxidase positive on SBAP

A

Aeromonas
Plesiomonas
Vibrio

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2
Q

Stools Bench:

what is SMAC plate used for and how does this organism look macroscopically?

A

E.coli O157

clear colorless colonies

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3
Q

Stools Bench:
what pathogens grow on MAC plate?
how do they look?

A 
salmonella 
shigella 
plesiomonas 
aeromonas 
Yersinia
(clear colorless)
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4
Q

Stools Bench:

what Pathogens grow on XLD and how do they look?

A

Shigella- red

Salmonellla-red with black center

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5
Q

Stools Bench:

what Media is used to isolate Campy?

A

CVA

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6
Q

Stools Bench:

What media is used to isolate and culture Vibrio? How does Vibrio look macroscopically on this media?

A

TCBS/vibro

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7
Q

Stools Bench:

Why do we incubate the MAC plate for 3 days?

A

Yersinia

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8
Q

P/0 Bench:

what are the stains used at this bench and what are they used for?

A
  • Trichrome stain
  • iodine
  • modified acid fast-isospora/cyclospora
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9
Q

P/0 Bench:

what pathogen is ID from corneal scrapings or contact lenses?

A

acanthomebia

-blinking on agar

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10
Q

P/0 Bench:

what 2 organisms are ID from a KIt test?

A

Crypto/Giardia

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11
Q

Wounds:

  • pleomorphic gram + rod
  • pinpoint colonies
A

cory

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12
Q

Wounds:
Pathogen of vulva
Gram + cocci

A

Group A strep

Strep. Pyogenes

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13
Q

meningitis in teens to mid twenties

A

Neisseria menigitidis

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14
Q

causes meningitis in young kids

A

Haemophilus influenzae

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15
Q

causes meningitis in old people

A

Strep pneumo

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16
Q

causes meningitis in neonates

A

Group B Strep

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17
Q

what are the Two Drug associated with the D test used for Pseudo?

A

Clindamycin and erythromycin

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18
Q

Anaerobes:
what is/ are the gram neg anaerobes?
**which is the only negative diplococci

A 
Veillonella parvula***
paraphyromonas spp.
prevotella melaninogenica
bacteriodes fragilis
fusobacterium spp.
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19
Q

Anaerobes:
what anaerobe is bile Esculine +
Resistant to Penicillin, Kenamycin, Vanco and Colliston?

A

Bacteriodes fragilis

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20
Q

Anaerobes:

what organism is black pigmented on Lacked Blood agar?

A

prevetella melaninogenica

21
Q

Anaerobes:

what gram neg cannot metabolize carbs?

A

Paraphyromonas spp.

22
Q

what common pathogens are Oxidase Positive?

A

Ps. aeruginosa
Aeromonas spp.
Vibrio

23
Q

Stools Bench:

How does E.coli look on TSI?

A

yellow slant/ yellow butt ,gas+

24
Q

Stools Bench:

How does Shigella sonnei look on TSI?

A

red/yellow no gas

25
Q

how does Salmonella enterica look on TSI?

A

red/yellow gas + black butt

26
Q

how does PSeudo look on TSI?

A

red/red

27
Q

Mycology:

what organisms is TBO stain used for?

A

-encapsulated organisms:

specifically Pneumocystis, Cryptococcus spp., Candida spp. ,Blasto

28
Q

Mycology:

what characteristic sets Cryptococcus neoformans apart from other Crypto?

A
  • pos bird seed agar test
29
Q

Mycology:

what organisms are urease pos?

A

H. Pylori and Cryptococcus spp.

30
Q

Mycology:

what candida spp. assilates trehalose?

A

candida glabrata?

31
Q

Mycology:

what dimorphic fungus is tuberculated at 30?

A

Histo

32
Q

Mycology:

what dimorphic fungus looks like a lollipop at 30?

A

Blasto

33
Q

Mycology:

what dimorphic fungus has a broad base mother cell that is 20 um at 37?

A

Blasto

34
Q

Mycology:

what dimorphic fungus is 70um spherale at 37?

A

cocci

35
Q

Mycology:

what dimorphic fungus is barrel shapped at 30?

A

coccidiodes immitis

36
Q

Mycology:
what class of fungus DO NOT GRIND??
-which all have this feature?
-what drug inhibits these?

A
  • Zygomyces
  • non-septate
  • cycohexamide
37
Q 
Mycology:
This class of fungus have a birds on a fence appearance
A

Dermatophytes

38
Q

Mycology:

what fungus do we use olive oil disks on plates to grow?

A

malizezia furfur?

39
Q

Mycology:

what dimorphic fungus is cigar chaped yeast?

A

sporothrix

40
Q

Mycology:

what dimorphic fungus has a yeast form that is red pigmented is promenint in S.E Asia and common in HIV patients?

A

Pen. marnefi

41
Q

Mycology:

why does CSF specimens only get BHI?

A

cryptococcus

42
Q

Mycology:

what plate is best to do lact blue off of?

A

PDA

43
Q

Mycology:

what lytic agent lysis RBCs when using the DuPont isolator?

A

supponin

44
Q

AST:

what are the antibiotics that pseudo is suscept. too?

A

aminoglycosides: gentamycin, ampicylin,

45
Q

what media is fastidious organisms plated too for AST and what is used to make the McFarland? instead of ?

A
  • Blood, BHI

- Mueller hinton, saline

46
Q

AST:

What drugs are used for E.coli?

A

Tempsola and ampcylin

47
Q

Anaerobes:
ric gram neg
pointed ends

A

Fuso

48
Q

what organisms are indole +

A

Proteus vulgaris.
Klebsiella oxytoca
E.coli

Clinicals Diag Micro (5 decks)

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