Final Exam Bench notes Flashcards
Stools Bench:
what enteric pathogens are oxidase positive on SBAP
Aeromonas
Plesiomonas
Vibrio
Stools Bench:
what is SMAC plate used for and how does this organism look macroscopically?
E.coli O157
clear colorless colonies
Stools Bench:
what pathogens grow on MAC plate?
how do they look?
salmonella shigella plesiomonas aeromonas Yersinia (clear colorless)
Stools Bench:
what Pathogens grow on XLD and how do they look?
Shigella- red
Salmonellla-red with black center
Stools Bench:
what Media is used to isolate Campy?
CVA
Stools Bench:
What media is used to isolate and culture Vibrio? How does Vibrio look macroscopically on this media?
TCBS/vibro
Stools Bench:
Why do we incubate the MAC plate for 3 days?
Yersinia
P/0 Bench:
what are the stains used at this bench and what are they used for?
- Trichrome stain
- iodine
- modified acid fast-isospora/cyclospora
P/0 Bench:
what pathogen is ID from corneal scrapings or contact lenses?
acanthomebia
-blinking on agar
P/0 Bench:
what 2 organisms are ID from a KIt test?
Crypto/Giardia
Wounds:
- pleomorphic gram + rod
- pinpoint colonies
cory
Wounds:
Pathogen of vulva
Gram + cocci
Group A strep
Strep. Pyogenes
meningitis in teens to mid twenties
Neisseria menigitidis
causes meningitis in young kids
Haemophilus influenzae
causes meningitis in old people
Strep pneumo
causes meningitis in neonates
Group B Strep
what are the Two Drug associated with the D test used for Pseudo?
Clindamycin and erythromycin
Anaerobes:
what is/ are the gram neg anaerobes?
**which is the only negative diplococci
Veillonella parvula*** paraphyromonas spp. prevotella melaninogenica bacteriodes fragilis fusobacterium spp.
Anaerobes:
what anaerobe is bile Esculine +
Resistant to Penicillin, Kenamycin, Vanco and Colliston?
Bacteriodes fragilis
Anaerobes:
what organism is black pigmented on Lacked Blood agar?
prevetella melaninogenica
Anaerobes:
what gram neg cannot metabolize carbs?
Paraphyromonas spp.
what common pathogens are Oxidase Positive?
Ps. aeruginosa
Aeromonas spp.
Vibrio
Stools Bench:
How does E.coli look on TSI?
yellow slant/ yellow butt ,gas+
Stools Bench:
How does Shigella sonnei look on TSI?
red/yellow no gas
how does Salmonella enterica look on TSI?
red/yellow gas + black butt
how does PSeudo look on TSI?
red/red
Mycology:
what organisms is TBO stain used for?
-encapsulated organisms:
specifically Pneumocystis, Cryptococcus spp., Candida spp. ,Blasto
Mycology:
what characteristic sets Cryptococcus neoformans apart from other Crypto?
- pos bird seed agar test
Mycology:
what organisms are urease pos?
H. Pylori and Cryptococcus spp.
Mycology:
what candida spp. assilates trehalose?
candida glabrata?
Mycology:
what dimorphic fungus is tuberculated at 30?
Histo
Mycology:
what dimorphic fungus looks like a lollipop at 30?
Blasto
Mycology:
what dimorphic fungus has a broad base mother cell that is 20 um at 37?
Blasto
Mycology:
what dimorphic fungus is 70um spherale at 37?
cocci
Mycology:
what dimorphic fungus is barrel shapped at 30?
coccidiodes immitis
Mycology:
what class of fungus DO NOT GRIND??
-which all have this feature?
-what drug inhibits these?
- Zygomyces
- non-septate
- cycohexamide
Mycology: This class of fungus have a birds on a fence appearance
Dermatophytes
Mycology:
what fungus do we use olive oil disks on plates to grow?
malizezia furfur?
Mycology:
what dimorphic fungus is cigar chaped yeast?
sporothrix
Mycology:
what dimorphic fungus has a yeast form that is red pigmented is promenint in S.E Asia and common in HIV patients?
Pen. marnefi
Mycology:
why does CSF specimens only get BHI?
cryptococcus
Mycology:
what plate is best to do lact blue off of?
PDA
Mycology:
what lytic agent lysis RBCs when using the DuPont isolator?
supponin
AST:
what are the antibiotics that pseudo is suscept. too?
aminoglycosides: gentamycin, ampicylin,
what media is fastidious organisms plated too for AST and what is used to make the McFarland? instead of ?
- Blood, BHI
- Mueller hinton, saline
AST:
What drugs are used for E.coli?
Tempsola and ampcylin
Anaerobes:
ric gram neg
pointed ends
Fuso
what organisms are indole +
Proteus vulgaris.
Klebsiella oxytoca
E.coli