FINAL EXAM Flashcards
What are you trying to prove with your experiment this semester?
That HP3 cell line will be Apoptotic when treated w/ Capsaicin.
NAME + DESCRIBE your Cell Line
HP3 - The cancer cell line that is widely used in research because of _____, _____, & _____.
NAME + DESCRIBE your Compound
Capsaicin - is a Metabolite of Chili Peppers & it is commonly used for ____, _____, & _____.
What is the Difference b/t/n Research PURPOSE + Research HYPOTHESIS?
The PURPOSE is WHY you are doing the experiment.
The HYPOTHESIS is WHAT you’re trying to prove.
What is the Central Hypothesis for your semester long project? (what did you think was going to happen with your experiment?)
Capsaicin will cause Cell Apoptosis.
What is a VEHICLE CONTROL?
a Vehicle Control is WHAT the Treatment/Substance of Interest is Administered in.
(e.g) - a hormone of interest is within a PBS solution and this is injected into the test group. Giving just the vehicle is to show that the response seen is due to the treatment being tested and not the solution it is in.
When would a VEHICLE CONTROL be added?
Depends on whether compound is Organic or Inorganic.
Did your Experimental Assays have a VEHICLE CONTROL? If so, Explain why.
Yes, our experimental assays used DMSO as a VEHICLE CONTOL b/c Capsaicin is an ORGANIC compound.
What are CONTROLS?
Control is the portion that you are NOT performing experiments on. It is just there to serve as the blank state that experiments compare to.
(e.g.) - in a drug trial, the control group are giving placebos and data from the experimental group that actually took the drug will be compared to it to see effects.
What is a POSITIVE (+) CONTROL?
POSITIVE (+) CONTROL = Something that SUPPORTS your HYPOTHESIS
What is a NEGATIVE (-) CONTROL?
The NEGATIVE CONTROL group is a group in which NO RESPONSE is EXPECTED.
What is the Difference b/t/n POSITIVE (+) CONTROL & NEGATIVE (-) CONTROL?
The NEGATIVE CONTROL group is a group in which NO RESPONSE is EXPECTED. It is the opposite of the POSITIVE (+) CONTROL, in which a KNOWN RESPONSE is EXPECTED.
What is a NEGATIVE (-) CONTROL?
The NEGATIVE (-) CONTROL group is a group in which NO RESPONSE is EXPECTED.
What is the Difference b/t/n POSITIVE (+) CONTROL & NEGATIVE (-) CONTROL?
The NEGATIVE (-) CONTROL group is a group in which NO RESPONSE is EXPECTED. It is the opposite of the POSITIVE (+) CONTROL, in which a KNOWN RESPONSE is EXPECTED.
My Hypothesis is —–. SO FAR my POSITIVE CONTROL is —– because it (either, Kills/Allows Cells to Proliferate.)
Capsaicin?
In your Project, what would Function as your NEGATIVE (-) CONTROL & your POSITIVE (+) CONTROL?
ie. Camptothecin, the Media, Which is positive /negative
WHY do we Conduct our Experiment more than once? (e.g. - In the Wound Assay, why did we “Scratch” cells w/in 3 wells instead of just 1 ? )
To yield more accurate results as well as test the variation from one to another.
WHY do we Conduct our Experimental Assays in Replicates? ( Why do we divide the Assays b/t/n Week 1 & Week 2?)
To test the Validity of the previous methods/conditions used from the prior week.
Which Assay DETERMINES the CONCENTRATION that your Reagent Kills?
EC-50?
Which Assay Utilizes FLUORESCENT MICROSCOPY?
Assay #5?
Which Assay DETERMINES that the Chosen Compound Affects the Ability of your CELLS to PROLIFERATE?
Assay #3?
Which Assay tested your ABILITY to PIPETTE?
Assay #1
