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Final Serology > Final > Flashcards

Final Flashcards

1
Q

Acute serological markers for hepatitis

A
  1. Anti-HAV
  2. HBsAg
  3. Anti-HBc
  4. Anti-HCV
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2
Q

Simultaneous affected with HepB and HepD

A

Co-infection

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3
Q

Pre-existing HepB then infected with HepD

A

Super infection

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4
Q

Anti-HBc Ab

A

HBV Ab marker for indicates Current/recent infection

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5
Q

Anti-HBe Ab

A

HBV Ab marker for rEcovery from infection

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6
Q

Anti-HBs Ab

A

HBV Ab marker for immunity to HBV (only seen in vaccinated people)

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7
Q

HBsAg

A

HBV Ag marker for active infection

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8
Q

HBeAg

A

HBV Ag marker for high degree of infectivity

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9
Q

What is the order of the serological markers in HBV?

A
  1. HBsAg
  2. HBeAg
  3. HBcAb
  4. HBeAb
  5. HBsAb
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10
Q

What are the acute serological markers for Hepatitis?

A
  • Anti-HAV
  • HBsAg
  • Anti-HBc
  • Anti-HCV
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11
Q

Excitation light

A

occurs at smaller wavelength and higher energy; incident

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12
Q

Emission light

A

higher wavelength and lower energy; fluorescent

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13
Q

Conventional microscope

A

uses light to illuminate the sample and produce a magnified image of the sample

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14
Q

Fluorescence microscope

A

uses a much higher intensity light to illuminate the sample

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15
Q

The antibodies used in emission light are labeled with what?

A

Fluorophores that can absorb light from incident light and convert into light with high wavelength and lower energy

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16
Q

Photoluminesence

A

Ability of living or non-living, organic or inorganic specimens to absorb and re-radiate light

17
Q

Epiflouresence

A

combination of excitation and emission wavelengths travel through a specimen, emitting fluorescene

18
Q

MEIA

A

microparticle EIA

19
Q

Nephelometry

A

Light scattering used to detect Ag/Ab complexes

20
Q

Competitive radioactive labels (RIA)

A

usually Ag is labeled (“tracer”)

21
Q

Noncompetitive RIA

A

Ab is labeled

22
Q

Enzymes labels (horseradish, alkaline, glucose)

A

Catalyzes substrates (OPD) and amplifies the signal

23
Q

FITC

A

common fluorescent label that is a compound that absorbs radiant energy at one wavelength and emit radiant energy at a longer wavelength

24
Q

Competitive immunoassay

A

Labeled Ag + unlabeled Ag + limited Ab

25
Q

Noncompetitive immunoassay

A

concentration of labeled Ag = bound Ab; sandwich assays that has wash steps

26
Q

Homogenous immunoassay

A

do not require separation of bound immune complexes from unbound complexes so limited washing

27
Q

Heterogenous immunoassay

A

require separation of bound immune complexes from unbound complexes so needs washing; better because you’re removing unnecessary stuff; uses solid phase component

28
Q

What immunoassay is used for drug detection?

A

EMIT

Final Serology (1 decks)

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