Final Flashcards
Acute serological markers for hepatitis
- Anti-HAV
- HBsAg
- Anti-HBc
- Anti-HCV
Simultaneous affected with HepB and HepD
Co-infection
Pre-existing HepB then infected with HepD
Super infection
Anti-HBc Ab
HBV Ab marker for indicates Current/recent infection
Anti-HBe Ab
HBV Ab marker for rEcovery from infection
Anti-HBs Ab
HBV Ab marker for immunity to HBV (only seen in vaccinated people)
HBsAg
HBV Ag marker for active infection
HBeAg
HBV Ag marker for high degree of infectivity
What is the order of the serological markers in HBV?
- HBsAg
- HBeAg
- HBcAb
- HBeAb
- HBsAb
What are the acute serological markers for Hepatitis?
- Anti-HAV
- HBsAg
- Anti-HBc
- Anti-HCV
Excitation light
occurs at smaller wavelength and higher energy; incident
Emission light
higher wavelength and lower energy; fluorescent
Conventional microscope
uses light to illuminate the sample and produce a magnified image of the sample
Fluorescence microscope
uses a much higher intensity light to illuminate the sample
The antibodies used in emission light are labeled with what?
Fluorophores that can absorb light from incident light and convert into light with high wavelength and lower energy
Photoluminesence
Ability of living or non-living, organic or inorganic specimens to absorb and re-radiate light
Epiflouresence
combination of excitation and emission wavelengths travel through a specimen, emitting fluorescene
MEIA
microparticle EIA
Nephelometry
Light scattering used to detect Ag/Ab complexes
Competitive radioactive labels (RIA)
usually Ag is labeled (“tracer”)
Noncompetitive RIA
Ab is labeled
Enzymes labels (horseradish, alkaline, glucose)
Catalyzes substrates (OPD) and amplifies the signal
FITC
common fluorescent label that is a compound that absorbs radiant energy at one wavelength and emit radiant energy at a longer wavelength
Competitive immunoassay
Labeled Ag + unlabeled Ag + limited Ab
Noncompetitive immunoassay
concentration of labeled Ag = bound Ab; sandwich assays that has wash steps
Homogenous immunoassay
do not require separation of bound immune complexes from unbound complexes so limited washing
Heterogenous immunoassay
require separation of bound immune complexes from unbound complexes so needs washing; better because you’re removing unnecessary stuff; uses solid phase component
What immunoassay is used for drug detection?
EMIT
