Final

Question 1

Abcissa, dependent variable

Answer 1

x-axis

Question 2

Increase working distance by

Answer 2

Decreasing mag power of objective lense

Question 3

Log x not log graph

Answer 3

semi-log scale

Question 4

Improve resolving power by changing light to

Answer 4

violet

Question 5

increase in numerical aperture improves

Answer 5

resolution

Question 6

monochromatic light

Answer 6

a single wavelength of light

Question 7

standard curve plots

Answer 7

absorbance vs. concentration

Question 8

y-axis

Answer 8

ordinant axis , independent variables

Question 9

why do we blank

Answer 9

to remove the background solution

Question 10

in cell fractionation we used

Answer 10

differential centrifugation

Question 11

homogenation

Answer 11

disrupts cell boundaries and releases cell contents

Question 12

nuclear fraction

Answer 12

pellet 1, contains nuclei intact cells and tissue

Question 13

post-nuclear supernant

Answer 13

produces the post-chloroplast supernant

Question 14

chloroplast fraction

Answer 14

Pellet 2, contains chloroplast and some organelles

Question 15

post mitochondrial supernant and mitochondrial fraction

Answer 15

pellet 3, smallest contains mitochondria, micro bodies and lysosomes

Question 16

smaller particles in supernant

Answer 16

greater sedimentation coefficient

Question 17

RPM

Answer 17

can be used stand alone

Question 18

RCF

Answer 18

need rotar size

Question 19

Ph

Answer 19

positive

Question 20

Ph>PI

Answer 20

negative

Question 21

Bromophenyl blue dye

Answer 21

tracker dye

Question 22

glycerol

Answer 22

weighs down the sample so it will stay in the well

Question 23

anions (-) travel towards

Answer 23

anodes (+)

Question 24

Native gel separates based on

Answer 24

charge, still have enzymatic activity

Question 25

glyciene

Answer 25

slow runner helps in stacking

Question 26

chloride ion

Answer 26

pushes sample down helps in stacking

Question 27

Non-native separates by

Answer 27

molecular weight

Question 28

Ammonium disulfate

Answer 28

isolates LDH

Question 29

APS

Answer 29

free radicles

Question 30

SDS

Answer 30

constant denaturing to linearize, all will have an overall negative charge. anionic detergent

Question 31

histochemistry

Answer 31

tissue staining

Question 32

4 steps in histotechnique

Answer 32

fixation sectioning staining mounting

Question 33

@low salt

Answer 33

solubility of protein increases, lower H2O solubility

Question 34

@high salt

Answer 34

solubility of protein decreases, higher H2O solubility

Question 35

BME

Answer 35

breaks di-sulfide bonds to linerarize( like chlorine)

Question 36

TEMED

Answer 36

polymerizing agent, works with/activates APS