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Cellular Physio Lab > Final > Flashcards

Final Flashcards

1
Q

Abcissa, dependent variable

A

x-axis

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2
Q

Increase working distance by

A

Decreasing mag power of objective lense

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3
Q

Log x not log graph

A

semi-log scale

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4
Q

Improve resolving power by changing light to

A

violet

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5
Q

increase in numerical aperture improves

A

resolution

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6
Q

monochromatic light

A

a single wavelength of light

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7
Q

standard curve plots

A

absorbance vs. concentration

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8
Q

y-axis

A

ordinant axis , independent variables

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9
Q

why do we blank

A

to remove the background solution

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10
Q

in cell fractionation we used

A

differential centrifugation

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11
Q

homogenation

A

disrupts cell boundaries and releases cell contents

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12
Q

nuclear fraction

A

pellet 1, contains nuclei intact cells and tissue

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13
Q

post-nuclear supernant

A

produces the post-chloroplast supernant

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14
Q

chloroplast fraction

A

Pellet 2, contains chloroplast and some organelles

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15
Q

post mitochondrial supernant and mitochondrial fraction

A

pellet 3, smallest contains mitochondria, micro bodies and lysosomes

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16
Q

smaller particles in supernant

A

greater sedimentation coefficient

17
Q

RPM

A

can be used stand alone

18
Q

RCF

A

need rotar size

19
Q

Ph

A

positive

20
Q

Ph>PI

A

negative

21
Q

Bromophenyl blue dye

A

tracker dye

22
Q

glycerol

A

weighs down the sample so it will stay in the well

23
Q

anions (-) travel towards

A

anodes (+)

24
Q

Native gel separates based on

A

charge, still have enzymatic activity

25
Q

glyciene

A

slow runner helps in stacking

26
Q

chloride ion

A

pushes sample down helps in stacking

27
Q

Non-native separates by

A

molecular weight

28
Q

Ammonium disulfate

A

isolates LDH

29
Q

APS

A

free radicles

30
Q

SDS

A

constant denaturing to linearize, all will have an overall negative charge. anionic detergent

31
Q

histochemistry

A

tissue staining

32
Q

4 steps in histotechnique

A

fixation
sectioning
staining
mounting

33
Q

@low salt

A

solubility of protein increases, lower H2O solubility

34
Q

@high salt

A

solubility of protein decreases, higher H2O solubility

35
Q

BME

A

breaks di-sulfide bonds to linerarize( like chlorine)

36
Q

TEMED

A

polymerizing agent, works with/activates APS

Cellular Physio Lab (1 decks)

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