Final

Question 1

Egg yolk agar

Answer 1

Detects Lipase, Lecithinase, and protease activities

Question 2

Which Clostridium spp has a double zone of hemolysis?

Answer 2

C. Perfringens

Question 3

Skim milk plates

Answer 3

Detects proteolysis of caesin protein by caseinase enzyme

Question 4

Spirit Blue Plates

Answer 4

Contains lipids and an indicator of pH for detection of lipase

Question 5

Nutrient Gelatin Tubes

Answer 5

Detects gelatin liquidification (gelatin protease activity)

Question 6

1% Tryptone broth

Answer 6

Used for detection of indole production by tryptophanase

Question 7

Litmus Milk Tubes

Answer 7

Provides lactose as a sugar source, Casein as a protein source, and looks for pH change

Question 8

McFarland Turbidity Standard

Answer 8

0.5 standard that contains 99.5 ml of 1% sulfuric acid and 0.5 ml 1.175% barium chloride and is used to dilute an inoculum until its turbidity matches the sample

Question 9

Mueller-Hinton Agar

Answer 9

Special agar for testing antimicrobial susceptability

Question 10

Minimum Inhibitory Concentration

Answer 10

A tube dilution method of measuring antimicrobial susceptibility. Measured by the lowest concentration of a drug that inhibits growth

Question 11

Minimal Bactericidal Concentration

Answer 11

The least concentration of an antimicrobial that is needed to kill 99.9% of organisms

Question 12

Potato Dextrose Agar

Answer 12

A high sugar growth medium used to grow fungi with a slightly acidic pH to inhibit bacterial growth

Question 13

10% KOH

Answer 13

Used in dermal isolates to dissolve keratinized cells leaving chitinous cell walls of fungi. Hyphae can be observed microscopically

Question 14

Lactophenol cotton blue

Answer 14

Stain for chitin

Question 15

Sabourad’s Agar

Answer 15

Agar with 4% glucose and a pH of 5.6 favor mold and inhibit bacteria

Question 16

Germ Tube Test

Answer 16

A 2 hour, sure fire way to ID pathogenic yeast. Combine with bovine serum, incubate and examine for germ tubes