Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BLOOD Bank > Final > Flashcards

Final Flashcards

1
Q

List the reagents and patient specimen used in an antibody screen.

A

Reagents: screening cells, LISS, and check cells
Specimen: patient serum or plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the purpose of the DAT, i.e. what does it detect?

A

Detects antibodies and complement attached to RBCs in vivo

– HDN, HTR, AIHA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

List the components of DAT

A
  1. patient cells

2. AHG

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

If a Rh control tube is necessary in the Rh testing, and it is positive, the interpretation of the Rh type is what?

A

Invalid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Name and differentiate the two types of AHG that can be used in BB

A
  1. Broad spectrum/ polyspecific: contains both anti-complement (C3d) and anti-IgG
  2. Monospecific:contains either, but not both
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the purpose of the IAT, i.e. what does it detect?

A

Detects antibodies and complement attached to RBCs in vitro

– used for XM and to screen cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

List the components of IAT

A
  1. patient serum

2. screening cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Name two blood bank procedures that incorporate the IAT

A
  1. crossmatching

2. Antibody screen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

List the steps in D^u testing, including the tubes included in the test.

A

Tubes: D test and Rh control test tubes

  1. Incubate both tubes at 37C for 15 mins
  2. Wash cells 3 times with saline
  3. add 2 drops of AHG to each tube and mix
  4. Centrifuge on high for 15 seconds
  5. Add 1 drop of check cells to any non-reactive tubes (results should be +)

(IWACA - to help remember the steps thanks to Angie!!!)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In the fetal bleed screening test, the presence of agglutinated cells indicates that the maternal blood contains a significant number of what?

A

Rh positive fetal cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What term is used to describe the agglutination that constitutes a positive test?

A

rosettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What test is used to determine the amount of fetal cells present in the maternal circulation.

A

Kleihaure- Betke Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

The AABB requires that Rh ____ mothers who have Rh ____ babies have the fetal bleed screening test performed.

A

Rh negative mothers with Rh positive babies.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

If a mother is Rh negative and has a baby that is Rh positive name three situations that may warrant the Kleihaure- Betke Test to be performed.

A

Abortion
trauma
miscarriage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

List 4 conditions that DAT can be used to detect.

A
  1. HDN
  2. AIHA
  3. HTR
  4. Drug-induced RBC sensitization
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

List the components of an auto control.

A

Patient red cells and

Patient serum

17
Q

Two cell populations in a patients specimen can produce _______ agglutination.

A

Mixed field agglutination

18
Q

The components of a forward ABO grouping are what?

A

Anti-A and anti-B antiserum and

patient cells.

19
Q

The components of a reverse ABO grouping are what?

A

Patient serum and

A1 and B cells

20
Q

What can be done to enhance an ABO reaction that is very weak upon immediate spin?

A

Sit at room temp for 30 mins or

Incubate at 4C

21
Q

What blood bank procedure is used to resolve a positive DAT?

A

Elation

22
Q

What blood bank procedure is used to resolve a positive antibody screen?

A

Antibody panel

23
Q

Clinically significant antibodies are Ig__ antibodies which like to react at ____C and in the ____ phase.

A

IgG
37C
AHG

24
Q

In a pregnant female, what antibody class is clinically significant?

A

Rh- IgG

Why? If the mother is Rh neg and baby is pos it could cause HDN, (sensitization of D antigen of fetal cells)

25
Q

What are the two AABB requirements for routine crossmatching?

A
  1. Demonstrate ABO incompatibility

2. Demonstrate clinically significant Ab

26
Q

The ____ is on the patient’s RBC; the ____ is in the patient’s serum

A

Antigen on RBC

Antibody in serum

27
Q

Why is the washing procedure before the addition of AHG so important?

A

To prevent neutralization of AHG by globulins in the blood

28
Q

FFP of the AB group is compatible with what blood group recipients?

A

All blood groups because AB plasma doesn’t have any antibodies

29
Q

Jk^a and Jk^b

Phenotypes #23

A

Pt 1: Jk(a= b=)
Pt 2: Jk(a+ b+)
Pt 3: Jk(a+ b=)

Pt 3 is heterozygous
Pt 2 is homozygous

30
Q

ABO compatible units # 24

A

O can only have O
B can have B and O
A can have A and O
AB can have A, B, AB, and O

(O is the universal donor where as AB is the universal recipient)

31
Q

Blood group types # 25

A

Blood group types # 25 1. O pos

  1. A neg
  2. B Rh control pos- unable to interpret
  3. A pos with possible A subgroup
  4. AB pos
  5. A neg with possible A subgroup or A transfused with O
32
Q

After the Coombs control cells are added to a negative AHG and the tube remains negative. What can explain this?

A

After the Coombs control cells are added to a negative AHG and the tube remains negative. What can explain this? 1. AHG was inactive
2. AHG was neutralized
Because RBCs not washed adequately
(Test needs to be repeated because the results are invalid)

BLOOD Bank (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions