Final Flashcards
What is the purpose of replication?
the process by which genetic information is duplicated. This occurs to ensure that each cell in an organism has a complete and correct copy of the genome
What is the purpose of transcriptions?
DNA is transcribing into mRNA
What is the translation?
mRNA is translated into a polypeptide chain with the aid of rRNA.
How does the DNA Polymerase complex work to replicate DNA?
By adding nucleotides one at a time to the 3’ end of a new strand, directed by the old template strand
A and G pairs with…
T and C
What is the difference between leading and lagging strands?
The leading strand is replicated continuously while the lagging strand is replicated discontinuously as Okazaki Fragments
What is the job of DNA Ligase?
It is and enzyme that can use ATP-Hydrolysis to link together breaks in the sugar-phosphate backbone
Where can DNA Polymerase add deoxynucleotides?
To the 3’ end and only if there is a sequence of nucleic acids to be copied on the 5’ side
Primase
synthesizes a new RNA primer
DNA Helicase
Unwinds the double helix
What is a gene?
Has 3 regions - Promoter region, Transcribed region and the terminator region
Promoter Region
1) Contains binding sites for transcription factors. 2) Contains binding sites for other factors that can un-pack or un-wind the chromatin. 3) Specifies where RNA Polymerase should begin transcription
Transcribed Region
1) The template strand of DNA (3’-5’) serves as a template to make an RNA copy of the non-template (5’-3’). 2) The RNA copy specifies where translation should start and end, also the amino acid sequence of the encoded protein.
Terminator Region
1) Contains binding sites for other factors that cause RNA Polymerase to stop transcription and leave the DNA. 2) Specifies the end of the RNA and how it should process the end
Introns
the non-coding part of the DNA that gets removed during the processing mRNA
Exons
The coding part of the DNA
mRNA
Encodes proteins and are translated by ribosomes
rRNA
Make up the majority of ribosomes, including most of the enzymatic functions
tRNA
Essential for translation of proteins
Eukaryotes
Multicellular organism (US!)
Caps and tails are added to…?
Eukaryotic RNA’s. Cap to 5’ and tail to 3’
How is the central dogma infiltrated?
DNA–transcription–>RNA–translation–>Polypeptide
How does the mRNA nucleotide sequence code for a protein amino acid sequence?
To code for 20 amino acids, need at least 3 nucleotides (4x4x4) = 64 codons. Thus a triplet code (3 nucleotides code one amino acid) seemed likely.
What are the stop codons for mRNA?
UAA, UAG, UGA
Point mutations
Caused by base-pair substitution, insertion, or deletion of nucleotides
Base -pair substituions are caused by
replication errors
Insertion or Deletion
Mutations caused by mistakes in repair or replication
Silent Mutation
don’t cause any changes in the encoded protein
Non-silent Mutation
Cause changes in coding. 3 types