final

Question 1

what is differential labeling

Answer 1

a method used to differently label 2 molecules of interest in an experiment so that investigators are able to distinguish btwn 2 molecules in the results

Question 2

what are 2 molecules that they were able to label in their experiment

Answer 2

dna and protein

Question 3

how did they label they molecule that was passed on to the next generation of phage

Answer 3

sulfur

Question 4

how did they label the molecules that were associated with phage ghosts after the experiment

Answer 4

P32

Question 5

what is the impact of having a nucleus on the creation of mature mrna in prokaryotes

Answer 5

because prokaryotes do not have a nucleus they translational machinery is able to attach to the mrna as it is being synthesized. this there is no time to process the mrna

Question 6

what is the impact of having a nucleus on the creation of mature mrna in eukaryotes

Answer 6

in eukaryotes the translational machinery is not present in the nucleus. thus there is time for the cell to process the mrna before it is transported from the nucleus to the cytoplasm and translated into protein.

Question 7

central dogma

Answer 7

replication, storage, expression

Question 8

what is the definition of biologically active dna in this context

Answer 8

dna that can be transcribed and translated and ultimately code for a viable protein.

Question 9

dna polymerase III adds nucleotides

Answer 9

to the 3’ end of the rna primer

Question 10

which of the following terms accurately describes the replication of dna in vivo ?

Answer 10

semidiscontinous

Question 11

which of the following enzymes are known to be involved in the replication of dna in bacteria ?

Answer 11

dna polymerase I, DNA ligase, rna primase

Question 12

which are characteristics of DNA sequence at the telomeres

Answer 12

• they consist of cytosine and adenine nucleotides
• the consist of repeated sequences
• one strand protrudes beyond the other, creating some single stranded dna at the end

Question 13

the term peptidyltransferase relates to

Answer 13

peptide bond formation during protein synthesis

Question 14

an intron is a section of

Answer 14

rna that is removed during rna processing

Question 15

in eukaryotes trna are

Answer 15

transcribed in the nucleus but function in the cytoplasm

Question 16

reverse transcriptase is an enzyme found in association with retroviral activity it has the property of

Answer 16

synthesis of dna from rna template

Question 17

What would Avery, Macleod, and McCarty have concluded if their results had been that only RNAse tréatment of the heat-killed bacteria prevented transformation of genetic virulence?

Answer 17

the rna is the genetic material

Question 18

which of the following statements best describes the mrna

Answer 18

mrna is the only type of rna that carries dna protein building instructions

Question 19

dna consists of repeating units of nucleotides. which is not a component of a nucleotide

Answer 19

a ribose sugar

Question 20

dna replication the lagging strand is

Answer 20

the strand which must be replicated In the direction away from the replication fork

Question 21

the spliceosome is a large enzyme that

Answer 21

catalyzes removal of specific introns in the pre mrna

Question 22

eukaryotic chromosomes contain two general domains that relate to the degree of condensation regions are

Answer 22

called heterochromatin and euchromatin

Question 23

what enzyme is exploited to produce synthetic mrna

Answer 23

polynucleotide phosphorylase

Question 24

the anti codon on the trna molecule

Answer 24

binds to the mrna in complementary fashion