Factors Affecting Enzyme Action Flashcards

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1
Q

What is used to measure the progress of an enzyme-catalysed reaction?

A

Time-course

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2
Q

What shows that all the substrate has been used in a graph showing enzyme reaction?

A

Graph line flattens out

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3
Q

What does an increase in temperature do to enzyme reaction rate?

A

Increases collisions

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4
Q

Does the rate of reaction increase or decrease as the temperature increases?

A

Increase

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5
Q

At what temperature do enzymes begin denaturing in humans?

A

45 degrees Celsius

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6
Q

What happens when an enzyme starts denaturing?

A

Bonds break and active site may change

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7
Q

At what temperature do enzymes denature?

A

60 degrees Celsius

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8
Q

Is denaturing permanent or reversible?

A

Permanent

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9
Q

Name one use of enzymes in the home in cleaning

A

Biological washing powder

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10
Q

What can cause the body temperature to change and denature enzymes?

A

Illness

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11
Q

What does a change in pH do to enzymes?

A

Changes the amino acids that make up the active site

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12
Q

Is the change in pH is significant enough what happens?

A

Bonds break and tertiary structure changes. Active site changes shape

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13
Q

What does a low enzyme concentration do to the reaction?

A

Too few enzymes to substrate molecules so rate of reaction is low

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14
Q

What happens in an intermediate enzyme concentration?

A

The substrate molecules can all occupy an active site and the ears of reaction is increased

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15
Q

What happens when there is a high enzyme concentration?

A

More enzyme that substrate so rate of reaction doesn’t increase past a certain point

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16
Q

What happens if there’s a low substrate concentration?

A

Too few substrate molecules for all available active sites