Factors Affecting Enzyme Action Flashcards
What is used to measure the progress of an enzyme-catalysed reaction?
Time-course
What shows that all the substrate has been used in a graph showing enzyme reaction?
Graph line flattens out
What does an increase in temperature do to enzyme reaction rate?
Increases collisions
Does the rate of reaction increase or decrease as the temperature increases?
Increase
At what temperature do enzymes begin denaturing in humans?
45 degrees Celsius
What happens when an enzyme starts denaturing?
Bonds break and active site may change
At what temperature do enzymes denature?
60 degrees Celsius
Is denaturing permanent or reversible?
Permanent
Name one use of enzymes in the home in cleaning
Biological washing powder
What can cause the body temperature to change and denature enzymes?
Illness
What does a change in pH do to enzymes?
Changes the amino acids that make up the active site
Is the change in pH is significant enough what happens?
Bonds break and tertiary structure changes. Active site changes shape
What does a low enzyme concentration do to the reaction?
Too few enzymes to substrate molecules so rate of reaction is low
What happens in an intermediate enzyme concentration?
The substrate molecules can all occupy an active site and the ears of reaction is increased
What happens when there is a high enzyme concentration?
More enzyme that substrate so rate of reaction doesn’t increase past a certain point