Factors affecting enzyme

Question 1

how does temperature affect enzymes?

Answer 1

> more heat = more K.E which makes the molecules move faster
this makes substrate molecules more likely to collide with enzyme active site.
Energy of collision increases, which means more succesful collision
Rate of reaction increases until enzyme reaches its optium temperature

Question 2

What happens if the temperature gets too high?

Answer 2

> The reactions stops as rise in temperature causes molecules to vibrate more
The vibration breaks some bonds that hold enzyme in shape
Active site shape changes so the enzyme + substrate don’t fit
Enzyme is denatured

Question 3

What happens if the pH goes above below the optium pH

Answer 3

> The H+ and OH- ions found in acids and alkali can break the ionic and hydrogen bonds that hod the enzyme tertiary structure in place.
This makes the active site change shape so the enzyme is denatured

Question 4

How does the enzyme conc affect?

Answer 4

> More enzyme molecules there are in solution, the more likely a substrate molecule is to collide with and form an enzyme - substrate complex
so increasing con increases rate of reaction

Question 5

how does the substrate concentration affect?

Answer 5

> The higher the substrate conc, the faster the reaction
more substrate = more collision between substrate + enzyme = more enzyme substrate complexes
until the enzyme conc becomes limiting factor