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AnaChem > (F) Oral reports > Flashcards

(F) Oral reports Flashcards

1
Q

Determines concentration of solute in solution based on the potential of electrochemical cells
(without drawing current); static conditions preserve analyte composition

A

Potentionmetry

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2
Q

Device that generates electricity from the
chemical reactions occurring in it

A

Electrochemical Cell

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3
Q

Detects specific ions or molecules in a
solution

A

Indicator Electrode

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4
Q

Measure of how much voltage exists between
2 half-cells

A

Cell Potential

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5
Q

Reference point for the potential difference
between the indicator electrode and solution

A

Reference Electrode

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6
Q

Site where oxidation occurs (electrons are lost
to the solution

A

Anode

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7
Q

Site where reduction occurs (electrons are
gained from the solution)

A

Cathode

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8
Q

Maintains electrical neutrality between
half-cells

A

Salt Bridge

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9
Q

T or F : the stronger the oxidizing agent, the more electrons it can accept

A

T

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10
Q

T or F: The weaker the reducing agent, the more electrons it can give away

A

F (the stronger)

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11
Q

Site of gaining electrons

A

Cathode

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12
Q

site of losing lectrons

A

anode

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13
Q

T or F : electrons flow from negatively charged going to positively charged)

A

True anode to cathode

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14
Q

this component of potentiometry prevents charge buildup, neutralizing both slutions and allows for a voltage to exist

A

salt bridge

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15
Q

studies absorption, emission, and scattering of EM radiation by particles in solid, liquid, or gas
form

A

Spectroscopy

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16
Q

measures radiation (via wavelengths) absorbed and transmitted by a sample (in
comparison to a reference/blank sample) to quantify its chemical properties

A

UV-Vis Spectroscopy

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17
Q

Lies between visible light and X-rays along the EM spectrum and can be seen and distinguished by the naked eye

A

Visible Light

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18
Q

wavelengths of visible light

A

400-700 nanometers

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19
Q

frequencies of visible light

A

400 THz to 800 THz

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20
Q

light sources : - first source, loud noise,
intensity decreases overtime

A

Deuterium Lamp

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21
Q

light sources : - second source, very
stable, has low noise and drift

A

Tungsten-Halogen Lamp

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22
Q

light sources : high-intensity, produces
more noise, less stable

A

Xenon Lamp (alt.)

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23
Q

Sample used for UV examination

A

quartz

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24
Q

sample that absorbs uv light

A

plastic

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25
Q

Detectors : amplifies ejected electrons to generate larger currents

A

photomultiplier

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26
Q

Detectors: allows electrons to flow through the semiconductor when exposed to light

A

photodiode detector

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27
Q

Provides energy to change the electronic state of a sample when it absorbs or emits EM
radiation

A

Light Sources

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28
Q

Light sources:- provides useful energy in the visible region of the EM spectrum

A

Deuterium Arc Lamp

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29
Q

Light sources: yields good intensity over the UV spectrum

A

Tungsten Lamp

30
Q

Separates wavelengths of light into a marrow (single-beam) which consists of the entrance and
exit slits

A

Monochromator

31
Q

Separates incoming beams into 2 cuvettes or cells

A

Beam splitter

32
Q

a solvent / blank solution

A

reference cuvette

33
Q

contains the solution or solid being analyzed

A

sample cuvette

34
Q

Transforms a light signal into an electrical signal; should have linear response with high
sensitivity and low noise

2 common types used are:
- Photodiodes
- Photomultiplier tubes

A

Detector

35
Q

Can be presented using a graph of Absorbance, Optical Density, or Transmittance (as a
function of the wavelength)

A

Data Processing/Analysis

36
Q

: Investigative process that utilizes fluorescence properties of samples for quantitative
measurements of chemical properties

A

Fluorescence spectroscopy

37
Q

absorb light energy of a specific wavelength and re-emit it at a longer
wavelengths

A

Fluorochromes/fluorophores

38
Q

T or F: Fluorochromes will not fluoresce if they are
illuminated with a light of corresponding wavelength
(depends on the absorbance spectrum); ensure that
enough energy is delivered to elevate the electrons into an
excited state

A

F (they will only fluoresce if the given criteria are met)

39
Q

Light wavelengths OVER the UV and visible range
can provide the excitation energy

A

Light Source

40
Q

Contains the experimentally needed chromatic
light (with a wavelength range of 10 nm)
- Prisms
- Diffraction gratings

A

monochromators

41
Q

used in addition to monochromators to purify the light

A

optical filters

42
Q

standard detector

A

InGAas array

43
Q

Lab technique that divides DNA, RNA, or protein combinations based on their molecular size

most difficult and expensive also - sabi ni mam

A

GEL ELECTROPHORESIS

44
Q

Carried out under constant voltage, current,
or power (migration rate should be
constant

A

Power Supply

45
Q

Establishes an electrical charge on the
system’s solute and pH

A

buffers

46
Q

Ca be in the form of slabs, sheets, and colums made out of starch, polyacrylamide, agarose, ad cellulose acetate membrane acting as a molecular sieve separating the molecules

A

support media

47
Q

T or F : Support media is in a crystalline form with more than 90% water

A

False it should be colloid

48
Q

Separates biomolecules based on
molecular size and surface charge

A

Starch Gel and DNA Ladder

49
Q

its a solution of DNA of different
lengths used as a reference to estimate the
size of unknown DNA molecules separated
based on their mobility in the electrical field

A

DNA Ladder

50
Q

Prepared by treating acetic anhydride with
cellulose
- Must be soaked in the buffer to get the
softened form and cleaned afterwards

A

Cellulose Acetate

51
Q

Allows DNA molecules to travel within it, the ends of the gel containing holes where DNA is placed`

A

agarose

52
Q

Accommodates large volumes of protein in
a single shot

A

Polyacrylamide

53
Q

T or F : separation is based on molecular weight and mass to charge ratio

A

True

54
Q

There is diffusion of large proteins

A

False as pore sizes are smaller

55
Q

A plastic box or tank filled with a buffer for
biomolecule migration

A

Electrophoresis Chamber

56
Q

Used to stain and destain gels
- Open-form and lidded boxes are available

A

gel containers

57
Q

T or F : it has 2 copper electrodes facilitating molecule separation by attracting opposite charges

A

False platinum

58
Q

T or F : anode binds to negative ions while cathode binds to positive ions

A

F (baliktad)

59
Q

Greek meaning below

A

INFRA

60
Q

What does INFRARED mean?

A

below red

61
Q

latin for image

A

sPECTRA

62
Q

means observation

A

skopia

63
Q

the analysis of infrared light interacting with a molecule through measuring absorption, emission, and reflection

A

infrared spectroscopy

eYYYYY REPORT NAMIN YAN

64
Q

Instrumentation: Measures the difference between the
amount of IR of a particular wavelength
generated by the lamp and the amount that
reaches the detector

A

non dispersive

65
Q

Examines IR radiation absorbed by the
sample
- Separated using a prism or grating
- Detectors are used to measure light
strength
- Is labor-intensive

A

Dispersive

66
Q

Analyzes the wavelengths or frequencies of
radiation absorbed by a sample

A

Fourier Transform

67
Q

T or F : In fourier transform, the mechanism of the moving mirror must be precise

A

T

68
Q

the x-axis

A

wavenumber

69
Q

y-axis

A

transmittance

70
Q

lowest part of the wave

A

trough

71
Q

(Rightmost side of the
graph where fluctuations are observed, unique for every element

A

fingerprint region

72
Q

ti

A

te

AnaChem (9 decks)

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