extracción DNA tecnica fenol cloroformo Flashcards

1
Q

paso 1

A

lisis de celulas y liberacion de DNA
lisis de eritocitos, membranas celulares y nucleares
cloruro de amonio

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2
Q

paso 2

A

extracción de proteinas y lípidos con solventes orgánicos
fenol
fase organica y acuosa

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3
Q

paso 3

A

Precipitación del DNA

solución salina y etanol 100%

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4
Q

paso 3

A

lavado de DNA
etanol 70
agua

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