Expression of recombinant proteins in E coli Flashcards
1
Q
name some protein-based therapeutics
A
hormones
cytokines
vaccines
monoclonal antibodies
2
Q
what amount of protein-based therapeutics are produced in E.coli?
A
1/3
3
Q
what is protein purification?
A
Purify a single protein from a mixture of proteins
4
Q
why is protein purification useful?
A
- Separation from proteins with similar function to measure activity/function of one particular protein
- Comparison of mutant proteins
- Structural studies (X-ray, NMR, Cryo-EM)
5
Q
what are some ‘old-fashioned’ methods for protein purifications that are still used?
A
New overexpression systems
Tag-based protein purification
Cell free expression systems
6
Q
A
gene transcribed by mrna. mrna is translated into protein by ribosome
7
Q
slide 18 and beofre
A
8
Q
slide 18 and beofre
A
