Exploring genes and genomes Flashcards
1
Q
What are some of the key techniques used in exploring the genome?
A
- Restriction-enzyme analysis
- Blotting techniques
- DNA sequencing
- Solid-phase synthesis of nucleic acids
- Polymerase chain reaction (PCR)
2
Q
What are restriction enzymes and how can they be used by scientists?
A
Enzymes that recognise specific base sequences in double-helical DNA and cleave, at specific places, both strand of that duplex.
Scientists can exploit this and cleave DNA into specified fragments that can be analysed.
3
Q
Restricton enzymes cleave what kind of sequences?
A
Palindromic sequences
4
Q
A
