Experimental Techniques Flashcards
Q: What is qualitative data in cell biology?
descriptive data that can provide insights into cellular behaviours
Q: What is the purpose of using Ki-67 in cell proliferation studies?
-Ki-67 is a protein expressed only in proliferating cells
-making it useful for detecting and visualizing cell proliferation through immunofluorescence.
Q: How can cell death be measured in dopaminergic neurons? By using viability markers that fluoresce upon entering dead cells
By using viability markers that fluoresce upon entering dead cells such as Sytox or propidium iodide
Q: What is the hypothesis regarding the drug xPD and dopaminergic neurons?
The hypothesis is that the addition of xPD reduces cell death in dopaminergic neurone
potentially addressing the loss of these cells in Parkinson’s disease.
Q: Describe the Transwell assay.
The Transwell assay is used to measure cell migration potential by allowing cells to migrate through a porous membrane in response to a chemoattractant.
Q: What is the significance of using DAPI in microscopy images? fluorescent stain that binds to DNA
-fluorescent stain that binds to DNA
-allowing visualization of all cells in a sample
Q: What does the experimental design for analyzing muscle cell proliferation with FGF-6 involve?
-Growing muscle cells with and without FGF-6 for 72 hours
-counting Ki-67 positive cells and total cells to calculate the percentage of proliferating cells.
Q: What are some examples of fluorescent dyes used to assess cell viability?
Examples include Sytox ethidium homodimer 1
Q: Why is it important to take multiple images in cell biology experiments?
Taking images from multiple locations increases the accuracy of data by providing a more representative sample of the cell population.
Q: What cellular behaviors can be studied in cell biology? A: Cellular behaviors include migration
Cellular behaviors include migration and invasion
Q: What is the role of the control group in experiments analyzing cell death?
The control group allows for comparison to determine the effect of treatments (like xPD) on cell viability and death rates.
Q: What is the observation related to lung cancer cells in migration studies?
-Lung cancer cells are found in other body locations
-this suggests they have a greater migration potential compared to normal lung cells.
Q: What is the significance of the FGF-6 growth factor in muscle regeneration? FGF-6 is hypothesized to enhance muscle cell proliferation
-FGF-6 is hypothesized to enhance muscle cell proliferation
-potentially improving muscle regeneration after injury.
