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BioChemMod6 > Experimental procedures for membrane proteins > Flashcards

Experimental procedures for membrane proteins Flashcards

1
Q

How can RBC be used to study membrane proteins?

A

Cytosolic side of the plasma membrane can be studied in RBC ghosts

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2
Q

How can mild detergents be used?

A

Used to solubilise, purify and reconstitute functional membrane proteins

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3
Q

What is Valinomycin?

A

Mobile carrier, carries potassium ions through the membrane .
A peptide ionophore
Carrier in Matthews

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4
Q

Terms explained

A

SIMPLE DIFFUSION = PASSIVE DIFFUSION • PASSIVE TRANSPORT = FACILITATED DIFFUSION = CARRIER MEDIATED TRANSPORT • PORE=CHANNEL • CARRIER = TRANSPORTER = PERMEASE (INTEGRAL MEMBRANE PROTEINS • IONOPHORE (CARRIER IN MATTHEWS) = SMALL MOBILE POLYPEPTIDE

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5
Q

Pore forming toxins

A

Alpha- Hemolysin from Staphylococcus aureus forms a transmembrane channel

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BioChemMod6 (8 decks)

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