Experimental Procedures Flashcards
How might a saturation screen be used?
Designed so that all of the genes in the genome are mutated at some point.
How does ChIP work (4 steps)?
- Cross-link DNA to protein
- Shred DNA
- Add anti-protein antibodies (on beads) and precipitate
- Sequence attached DNA
When would you want to utilize genetic linkage mapping?
When you want to determine the gene which causes a particular condition by first identifying the chromosome and then candidate genes.
How does the GAL4 UAS system work in drosophila?
Cross one fly with upregulated expression of GAL4 and another fly with a UAS activated by GAL4 that will cause expression of any target gene (ex: GFP, etc.).
Why would we want to use fate-mapping?
To determine what the cell we are looking at now becomes later on in development.
What can β-galactosidase-expressing LacZ be used for?
As an indicator of gene expression if inserted into gene of interest.
Why would we want to use the CRE-Lox system?
When we only want to control gene expression at a certain time.
What are the advantages of using chick or zebrafish as a model organism?
Because they are transparent during early development.
Why might Aplysia (sea slug) be a good model organism for studying Axon Guidance?
Simple nervous system and huge neurons, so easy to examine in culture.
Why is a chick an excellent model organism for visualizing limb development?
Because it’s easy to access and manipulate their developing limbs. (also transparent)
Under what circumstances would we want to use “nude mice” as a model organism?
Any time we want to insert tissue into a mature organism. “Nude mice” lack T cells and so won’t reject the donor tissue.
