Experimental Procedures Flashcards
1
Q
(trans)dermal application
A
- drug in solution applied directly on (shaven) skin or mucous membrane
- not accurate, variability in skin penetration &risk of rubbing, licking
2
Q
enteral administration of compounds
A
- oral administration to the gastrointestinal tract
- e.g. through food & drinking water
- not possible with unpalatable substances
3
Q
Types of injections
A
- intracutaneous (intradermal)
- subcutaneous
- intramuscular
- intraperitoneal
- intravenous
4
Q
Attention to features of injection techniques:
A
- width (gauge) of needle
- sterility
- max volume of injection
- air bubbles
- injected fluids must be brought to room temp (cold fluids are painful)
- IP inj risk of damaging internal organs
- high or low pH fluids should be diluted in saline
- large amounts of fluid should be adminstered IV, IP or orally
5
Q
Maximum volume of blood to be collected
A
- total volume is constant at ca. 8% of body weight for most animals
- > 10% of blood volume sample weight can lead to hypovolaemia and cardiovascular failure (shock)
- rule of thumb: max 8ml/kg of bodyweight
6
Q
Suturing techniques
A
- interrupted stitch vs continuous stitch (continuous is faster but not recommended for larger wounds as rupture can result in the entire stitch unravelling)