Experimental Design Flashcards
What is the observed signal from a pH meter?
The volume of NaOH or Hcl Added to the reaction mixture
What are the three main steps of quenching a reaction?
- initiate reaction (trigger)
- Quenching
- separating and quantifying components
why does a fluorimeter have two monochromators?
Because the fluorophore absorbs light of one wavelength and emits it at another so the light needs to be directed and regathered towards the photomultiplier
What does surface plasmon resonance measure?
The relative Kd of a ligand binding a protein (via refractive index)
In what circumstances would you use radioactivity to assay a reaction?
If you required a highly sensitive technique
What is an example of a molecule which can be used with flash photolysis
Caged ATP
What features make HPLC more accurate than normal chromatography?
stronger column matrices, automatic injection and high pressure.
What is the function of a scintillant?
A scintillant is added to a quenched reaction component to convert radiation energy to light quanta
What feature does the platinum ball give to continuous flow?
Two reactants can be pushed against the platinum ball to mix them at very high speeds - allows quantification of very fast reaction times
In what circumstance would you use a quenched flow technique?
When the reaction components need to be separated to receive a clear signal
