EXPERIMENT 5

Question 1

this produces spores

Answer 1

SPORE FORMERS

Question 2

resistant to heat to any environmental condition

Answer 2

SPORE FORMERS (spore-forming)

Question 3

This is very difficult to kill with simple physical agent

Answer 3

SPORE FORMERS (spore-forming)

Question 4

What are the materials used for Growth inhibition and destruction of bacteria by physical agents

Answer 4

Suspensions of Bacillus subtilis and Escherichia coli

Filter paper discs impregnated with Bacillus subtilis and Escherichia coli

Nutrient broths

Question 5

This cannot resist harsh environmental conditions

Answer 5

VEGETATIVE BACTERIA

Question 6

How many nutrient broths were prepared for the experiment on Growth Inhibition and Destruction of Bacteria
by Physical Agents

Answer 6

5 nutrient broths on different test tubes

Question 7

How were the 5 test tubes with nutrient broths labeled

Answer 7

a. Autoclave
b. Hot air
c. Cold
d. boiling
e. Control

Question 8

What is the purpose of nutrient broths?

Answer 8

They are general purpose broths and used for routine culture. Non-selective (promotes growth of any microorganisms)

Question 9

Is nutrient broth selective or non-selective

Answer 9

NON-SELECTIVE

Question 10

When acquiring specimen from liquid suspension what material should be used?

Answer 10

Wire loop

Question 11

What is the mechanism of action in sterilization with moist heat?

Answer 11

Protein coagulation

Question 12

Name the three types of sterilization with moist heat?

Answer 12

1. Boiling
2. Steam under pressure or autoclave
3. Fractional / intermittent sterilization
4. Pasteurization or tyndallization

Question 13

What temperature should be used for sterilizing through boiling

Answer 13

100 degrees Celsius

Question 14

How long should the sterilization through boiling be done?

Answer 14

30 minutes

Question 15

What are the advantages of using boiling as a sterilization with moist heat method

Answer 15

It is the most inexpensive and the most practical

Question 16

What is the disadvantage of boiling as the method for sterilization with moist heat?

Answer 16

It is not effective in killing spores

Question 17

For the suspensions in Growth inhibition and destruction of bacteria by physical agents, what staining was used?

Answer 17

Gram staining

Question 18

For the control test tube, what temperature should it be incubated at?

Answer 18

37 degrees Celsius

Question 19

This sterilization method is considered the most powerful heat sterilizing agent

Answer 19

STEAM UNDER PRESSURE or AUTOCLAVE

Question 20

What is another name of the sterilization method “Steam under pressure”

Answer 20

Autoclave

Question 21

What temperature should be set when using the autoclave or the steam under pressure method?

Answer 21

121 degrees celsius

Question 22

How long should the autoclave method be done?

Answer 22

At least 15 - 20 minutes

Question 23

The amount of pressure to be placed when doing the autoclave method.

Answer 23

15 lbs. pressure

Question 24

What is/are the advantage/s of using the autoclave method?

Answer 24

a. All microorganisms and their endoscopes are destroyed

b. Shorter time is required compared to the dry heat sterilization

Question 25

What type of microorganism cannot be killed using the autoclave method?

Answer 25

The prions