Experiment 4 part 1

Question 1

Assay

Answer 1

• measurement of enzyme activity

Question 2

Chromogenic substances

Answer 2

• products converted by enzyme which are easily detected by a spectrophotometer
• substrate= colourless
• product = colour

Question 3

Substrates In experiment

Answer 3

BA - D, L -arginine p-niteoanilide hydrochloride ( trypsin more specific to arginine side chain )
S- phenylalanine-p-nitroNilide ( chymotrypsin specific side chain )

Question 4

Zymogen activation

Answer 4

• mode of enzyme regulation (ex digestive, blood clotting , activate enzymes for programmed cell death )

Question 5

Zymogen

Answer 5

• inactive form of enzyme

Question 6

Zymogen in experiment

Answer 6

Zymogen-a-chymotrypsinigen (converted to to active a-chymotrypsin by trypsin)