Experiment #1 pH and Buffers

Question 1

a measure of hydrogen ion concentration

Answer 1

pH

Question 2

Samples used in the lab for pH determination

Answer 2

Urine
Fresh milk

Question 3

Normal pH of
pure water
blood
fresh milk
urine

Answer 3

pure water = 7
blood = 7.35-7.45
fresh milk =7
urine = 6 (1-6.4)

Question 4

ph ___ numerically as the acidity of the solution _______

Answer 4

ph decreases

solution increases

Question 5

Litmus Paper

Answer 5

Red-red solution red = Acid solution

blue-remains blue in basic solution = basic solutions

Question 6

solutions of combinations that resist change in acidity or basicity

Answer 6

Buffer Soultion

Question 7

solutions of a weak acid with the salt of the weak acid, or of a weak base with a salt of the base, usually serve as a buffer solution
(true or false)

Answer 7

true

Question 8

hydrochloric acid + acetic acid and sodium acetate

Question 9

a number of buffer systems in the body to assist in maintaining the nearly constant pH of the blood
(true or false)

Answer 9

true

Question 10

the phenomenon in which the solvent passes through a semipermeable membrane from a solution of LESSER CONCENTRATION to one of greater concentration

Answer 10

Osmosis

Question 11

osmosis is necessary for the proper functioning of living matter (true or false)

Answer 11

true

Question 12

the pressure that must BE EXERTED to stop the flow of water across the selectively permeable membrane by osmosis

Answer 12

Osmotic Pressure

Question 13

The three types of osmotic conditions include-

Answer 13

hypertonic, isotonic, and hypotonic.

Question 14

when a solution has the same osmotic pressure as some body fluid
NO CHANGE IN SIZE OR SHAPE

Answer 14

ISOTONIC

Question 15

a physiological salt solution (0.85%-0.90% sodium chloride)

Answer 15

Isotonic

10% salt
salt and water
comparing it to the blood plasma
Osmotic pressure = blood plasma

Question 16

red blood cells burst if lower osmotic pressure

water flows into these cells FASTER THAN IT FLOWS out of them

Answer 16

Hemolysis

hemo - blood
lysis - burst separates divides

Question 17

when a solution has lower osmotic pressure than the blood plasma
SWELL

Answer 17

Hypotonic

Question 18

a solution that has a higher osmotic pressure than the blood plasma

if placed in the blood plasma/red blood cells
SHRINK OR CRENATE

Answer 18

Hypertonic

Question 19

a measure of the attractive forces exerted among molecules at the surface of a liquid

Answer 19

surface tension

Question 20

what is used sample in osmosis

Answer 20

Defibrinated blood

Reagent
0.9% NaCI
0.1 M NaCI
1.0 M NaCI

Question 21

what is used as test solutions in buffer solution

Answer 21

Test Solution:
Distilled water
Sodium acetate solution
Phosphate buffer
Albumin solution

Question 22

What is added in the samples buffer solution

Answer 22

pH + pOH = 14

(Strong Acid) HCI Hydrochloric acid
(Strong Base) NaOH Sodium hydroxide

Question 23

Satisfactory Buffer solution

Answer 23

Acetic Acid - Sodium Acetate
Ammonium chloride - Ammonium hydroxide
Potassium Dihydrogen Phosphate - Dibasic monohydrogen phosphate

Question 24

most molecules are transported across biological membranes by specific protein carriers called as ___

Answer 24

permeases

Question 25

Dialysis Chemical tests what are reagents added and how to know the results

Dialysate for testing
fresh milk
cellophane
distilled water