Exercise 14

Question 1

conjugation

Answer 1

Direct cell to cell contact involving the formation of a conjugation bridge, allowing the host cell to transfer plasmid DNA to recipient

Question 2

Donor strain (F+)

Answer 2

Has plasmid DNA (Ec2). can make the sex pili. male. donor

Question 3

Recipient Strain (F-)

Answer 3

only has chromosomal DNA. can not transfer or make sex pili

Question 4

Chromosomal DNA

Answer 4

linear shape. does not transfer during conjugation

legit DNA

Question 5

Plasmid DNA

Answer 5

Circular pieces of DNA that exist and replicate independently of the chromosomal DNA. Carries the R factor, the genes that confer resistance to antibiotics. Often have enzymes that chemically inactivate the drug. not required like chromosomal DNA. can only benefit the cell in limited set of environments, none encode essentials cellular functions

Question 6

What type of genes are found on plasmids?

Answer 6

the R factor that is resistance to antibiotics

Question 7

What type of DNA is transferred from an F+ strain?

Answer 7

plasmid DNA

Question 8

What resistance markers were used on this experiment and which strains had them?

Answer 8

streptomycin Ec1

ampicilian Ec2 naldixic Ec2

Question 9

what is a recombinant?

Answer 9

the result of F+ and F-, typically stronger when it comes to resistant against antibiotics than the parents. or much weaker.

Question 10

How was the recombinant used to verify the identities of the parent strains?

Answer 10

it was resistant to streptomyocin and ampicilin (Ec1 resistant to strep and Ec2 resistant to ampicillin)