Exercise 1 Flashcards
Laboratory Tools and Equipments (16)
- _________: Sterilizes equipment and materials using high-pressure steam to kill microorganisms.
- _________: Preserves samples or reagents at low temperatures to prevent degradation or spoilage.
- _________: Removes moisture from samples or materials by heating them in an oven, typically at a controlled temperature.
- _________: Provides a controlled environment for safely conducting experiments with volatile or hazardous substances by containing and venting fumes.
- _________: Heats materials to high temperatures for various purposes such as annealing, sintering, or decomposition.
- _________: Stores moisture-sensitive materials in a dry environment to prevent absorption of moisture.
- _________: Used for electrophoresis to separate and analyze DNA, RNA, or proteins based on their size and charge.
- _________: Concentrates or purifies solutions by evaporating solvent under reduced pressure while rotating the sample.
- _________: Cuts thin sections of samples, typically biological tissues, for microscopic examination.
- _________: Maintains a constant temperature for incubating samples or conducting reactions that require precise temperature control.
- _________: Measures the mass of substances accurately for various applications including preparing solutions or determining sample quantities.
- _________: Measures the absorption or transmission of light by a sample at different wavelengths, used for quantifying concentrations or characterizing substances.
- _________: Removes moisture from samples by freezing them and then subjecting them to a vacuum, preserving the sample’s structure and properties.
- _________: Separates components of a mixture based on their density using centrifugal force, commonly used for isolating cells, proteins, or nucleic acids.
- _________: Magnifies and resolves the details of microscopic objects or specimens for observation and analysis.
- _________: Stores perishable materials or samples at low temperatures to prolong their shelf life or maintain their stability.
- autoclave
- freezer
- ovendry
- fumehood
- furnace
- dessicator
- bolt mini gel tank
- rotary evaporator
- microtome
- water bath
- balance
- spectrophotometer
- freeze dryer
- centrifuge
- microscope
- refrigerator
used to wipe down laboratory table/workbench and the stage of dissecting scope, as well as its knobs, and the instrument tray
70% alcohol
______ are importance tools to dissect embryos in any species and it should include (5)
dissecting tools:
- pair of scissors, forceps, knives, probes, and scalpels
most tools can be sterilized by (4)
- soaking in 70% alcohol for 10 minutes (most tools)
- placing in boiling water
- UV fluorescent bulb
- autoclaving
T or F
some researchers prefer to sandwich their instruments between folds of a paper towel that has previously been soaked in 70% alcohol instead of using an open instrument tray
True
is an exquisitely refined, expensive piece of equipment
compound microscope
is a specialized precision cutting instrument, which accurately and repeatedly slices sections from a block of a tissue.
microtome
different kinds of microtomes are used to section __________ and ___________ as well as the specialized microtomes used to section _________.
paraffin
plastic embedded tissue
frozen tissues
In any microtome, a _________ and the ______ are held in fixed relation to each other.
sharp knife and the tissue block
___________: 8-15 mm section thickness
___________: 4-10 mm
___________: 0.5-3 mm
frozen section
wax section
plastic section
