exer 2 pa lang ni.. hindi ko na alam

Question 1

unsa tong letter A to E be

Answer 1

A. preparing a smear
B. simple staining
C. differential stain - Gram staining
D. differential stain - Shaeffer Fulton Staining
E. Negative Staining

(dakoa ang G kay malian nasad :D)

Question 2

unsay ginabuhat sa preparing a smear

Answer 2

• mix small amount of bacterial medium onto a drop of mounting medium
• spread thinly on center of slide
• fix smear by passing it gently over flame (2 to 3 times) AYAW SUNUGA alangan naunsa diay ka

Question 3

oras para ihulat ang dye sa simple staining before rinsing off

Answer 3

3-5 min.

Question 4

4 steps of gram staining

Answer 4

1. primary stain
2. mordant
3. decolorizing
4. counterstain

Question 5

primary stain sa gram staining lab time

Answer 5

ammonium crystal 1 min.

Question 6

mordant sa gram staining lab time

Answer 6

Gram’s iodine 1 min.

Question 7

decolorizing agent sa gram staining

Answer 7

95% 15 seconds

Question 8

counterstain sa gram staining

Answer 8

safranin sol. 30 seconds

Question 9

used to stain endospore

Answer 9

Shaeffer Fulton Staining

Question 10

primary stain of Shaeffer Fulton staining

Answer 10

malachite green 7min.

Question 11

counterstain of Shaeffer Fulton staining

Answer 11

safranin solution 30 to 60 seconds

Question 12

stain used in negative staining

Answer 12

nigrosin

Question 13

unsay ginabuhat sa negative staining

Answer 13

• ibutang ang stain evenly
• tanggalon ang excess
• DILI HUGASAN alangan unsay point sa pag stain kung hugasan
• tapos ipauga dayon alangan lisod pod

Question 14

microorganisms used in exercise 2

Answer 14

• Bacillus subtilis (all)
• Escherichia coli