Exchange of substances- Topic 3 Flashcards
plasma membrane
the membrane around organelles and cells
functions of plasma membrane
Cell signalling and recognition
Important for recognition by neurotransmitters and hormones
Isolates the contents of the cell and organelles
Entry and exit of substances
The fluid mosaic model of the plasma
membrane
Mosaic because proteins in the membrane are dotted around in a mosaic pattern. Fluid because the proteins and phospholipids are constantly moving.
phosphate head
polar and hydrophilic
fatty acid tails
non-polar and hydrophobic
cholesterol function in membranes
controlling membrane fluidity. It does this by
fitting between the phospholipids and binding to the tails, which results in their movement being restricted. The more cholesterol, the less fluid – and the less permeable –
the membrane.
integral/ transmembrane proteins
span the whole width of the
membrane.
peripheral/ extrinsic proteins
confined to the inner or outer surface of the
membrane, usually receptors or involved in cell recognition
glycoproteins
proteins with attached carbohydrate chains
carrier molecules or channels
integral proteins that transport substances such as ions, sugars and amino acids these cannot diffuse across the membrane but are still vital to a cell’s functioning.
simple diffusion
the net movement of molecules or ions from a region where they are at high concentration to a region of low concentration. It is a passive process
passive process
does not require energy from ATP
rate of diffusion depends on
1.the concentration gradient
2.the distance over which diffusion occurs
3.the area over which diffusion occurs
4.the size and nature of the diffusing molecule
5.the size of the pores over which diffusion occurs
6.the temperature
which molecules can diffuse directly through the plasma membrane
non-polar and lipid soluble molecules
facilitated diffusion
diffusion which takes place through transmembrane channels and carriers. needed for polar and lipid insoluble molecules. Passive process.
Channel proteins
for water soluble molecules. Some channels are open all the time others open when a hormone is attached.
Carrier proteins
change shape when molecules bind with them allowing the molecule to diffuse through the membrane.
osmosis
The passage of water from a region where it has a higher water potential to a region where it has a lower water potential through a partially permeable membrane
partially permeable membrane
permeable to water molecules and a few other small molecules, but not to larger molecules
Water potential
the pressure created by water molecules, measured in kiloPascals (kPa).
highest water potential
0
more negative (lower) water potential
more concentrated solution
isotonic solutions
have the same water potential
water moves from a ….. water potential to a ……. water potential in osmosis
higher, lower
dynamic equilibrium
no net movement
active transport
The movement of molecules or ions into or out of a cell from a region of lower concentration to a region of higher concentration (against concentration gradient) using ATP and carrier protein
ATP——>
ADP + Pi
steps of active transport
1) molecule binds to receptor sites on carrier protein
2) on the inside ATP binds to protein
3) this splits ATP causing the shape of the protein to change so it opens on the other side
4) molecule released through open side
5) Pi released so protein returns to original shape. Pi recombines with ADP
Sodium Potassium pump
Na+ moves out of cell, K+ taken into cell
Co-transport
bind two molecules at a time, one moves down its concentration gradient and this is used to move the other molecule across the membrane against its concentration gradient
sodium glucose co transport in small intestine
1) Na+ ions actively transported out of epithelial cells by Na+/K+ pump
2) creates a concentration gradient
3) concentration gradient causes Na+ ions to diffuse from the ileum lumen into epithelial cells through Na+/glucose co transporter
4) glucose enters the cell with Na+
dilution series
A dilution where successive concentrations
increase/decrease in a logarithmic fashion
affect of increased temperature on phospholipid bilayer
Phospholipids gain kinetic energy, vibrate more and drift apart leaving gaps. Membrane proteins become denatured at high temperatures
practical to test membrane permeability
beetroot in different concentrations of alcohol or different temperatures
affect of ethanol on phospholipid bilayer
The embedded cell membrane proteins are denatured by the ethanol molecules causing the membrane to lose it’s integrity and become more leaky
surface area of a sphere
4 x pi x r^2
surface area of a cylinder
2 x pi x r x h + 2 x pi x r^2
volume of a sphere
4/3 x pi x r^3
large surface area to volume ratio
beneficial as short diffusion distance so quick
features of a gas exchange system
large SA to volume ratio, thin to shorten diffusion pathway, selectively permeable