Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbial Quiz 2 > Excerise 6 > Flashcards

Excerise 6 Flashcards

1
Q

What are the 2 major categories in bacteria ?

A

Gram positive and gram negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is it called to distinguish two groups of bacteria?

A

A differential stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is gram positive bacteria? (4)

A

Thick, single layered cell wall
Made of peptidoglycan
Purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is gram negative ? (4)

A

Multilayered cell wall
Thin layer of peptidoglycan
Surrounded by a LPS outer membrane
Pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What does LPS mean for gram - ?

A

Lipopolysaccharide containing outer membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the 3 dyes?

A

Crystal violet
Iodine
Safranin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What’s the 1 decolorizer?

A

Ethanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the 4 steps of a gram stain?

A

Crystal violet ( purple dye)
Iodine ( brown color )
Ethanol ( 95% alcohol )
Safranin ( red )

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What does the 95 ethanol do to a gram negative bacteria?

A

They lose the bluish purple color and become decolorized

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Some gram positive bacteria, may lose their characteristic in older cultures and appear gram negative? What bacteria?

A

Bacillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is gram variability?

A

Identification criterion that more needs to be done to figure out what bacteria it is

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is micrococcus luteus?

A

Gram positive coccus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is Escherichia coli?

A

Gram negative rod

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is staphylococcus?

A

Gram positive gram coccus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Streptobacillus?

A

Gram negative rods

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What’s the full name for gram iodine?

A

Iodine postassium iodide solution

17
Q

What does the iodine do to the crystal violet?

A

The dye complexes with the crystal violet to make a larger complex

18
Q

What happens to a gram positive bacteria when 95 ethanol is added?

A

Dehydrated and the dye is trapped inside the cell

19
Q

What happened to a gram negative when 95% ethanol is added?

A

Outer LPS is dissolved

20
Q

In order to visualize the gram negative cells what do we use?

A

Red dye called safranin ( counter stain )

21
Q

What does the crystal violet do?

A

Stains both bacteria purple

Microbial Quiz 2 (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions