Examination of Fresh Tissue

Question 1

Is the microscopic study of the normal tissues of the body

Answer 1

Histology

Question 2

Is the microscopic study of tissues affected by disease

Answer 2

Histopathology

Question 3

The simplest, least invasive test and uses the smallest needle to simply remove cells from the area of abnormality

Answer 3

Fine Needle Aspiration

Question 4

It removes not only cells, but also a small amount of the surrounding tissue.

Answer 4

Core Needle Biopsy

Question 5

Only a small portion of lesion is sliced or removed from a tissue

Answer 5

Incisional Biopsy

Question 6

Removal of the entire area in question

Answer 6

Excisional Biopsy

Question 7

Considered the primary technique for obtaining diagnostic full-thickness skin specimen.

Answer 7

Punch Biopsy

Question 8

Small fragments of tissue are scraped from a surface (e.g. skin)

Answer 8

Shave Biopsy

Question 9

Tissue is scooped or spooned to remove tissue or growths from body cavity (eg. Endometrium or Cervical canal)

Answer 9

Curettings

Question 10

Autolysis is retarted by ___________ and accelerated at _____________.

Answer 10

Cold, Room Temperature

Question 11

A process whereby a selected tissue specimen is immersed in isotonic salt solution; carefully dissected with a needle and separated by direct or zigzag spread using an applicator stick.

Answer 11

Teasing or Dissociation

Question 12

Process where small pieces of tissue not more than 1mm in diameter are placed in a microscopic slide and forcibly compressed with another slide or with coverglass

Answer 12

Squash preparation/Crushing

Question 13

Normally utilized when a rapid diagnosis of the tissue in question is required

Answer 13

Frozen Section

Question 14

It is recommended when lipids and nervous tissue elements are to be demonstrated

Answer 14

Frozen Section

Question 15

It is useful in cytological examinations, particularly for cancer diagnosis

Answer 15

Smearing

Question 16

What are the four Smearing Techniques

Answer 16

Streaking
Spreading
Pull-apart
Touch Preparation/Impression Smear

Question 17

A smearing technique where using an applicator stick, the material is rapidly and gently applied in a direct or zigzag line throughout the slide to obtain a uniform distribution.

Answer 17

Streaking

Question 18

A selected portion of the material is transferred to a clean slide and gently spread into a moderately thick film by teasing the mucous strands with an applicator stick.

Answer 18

Spreading

Question 19

It is done by placing a drop of secretion or sediment upon one slide and facing it to another clean slide

Answer 19

Pull-Apart Technique

Question 20

A special method where slide surface is in contact and pressed on the site

Answer 20

Touch Preparation/ Impression Smear

Question 21

Fresh tissue on a frozen section is kept at an atmospheric temperature of _________ to __________.

Answer 21

-10°C, -20°C

Question 22

What is the advantage of frozen section method?

Answer 22

Rapid processing with less equipment requirement.

Question 23

The most rapid of the commonly available freezing agents

Answer 23

Liquid Nitrogen

Question 24

Majority of non-fatty unfixed tissues are sectioned well at temperatures between _______ and _______

Answer 24

-10°C, -25°C

Question 25

How to fix vapor phase formation when using a liquid nitrogen?

Answer 25

Freezing tissue in Isopentane, OCT, Freon 2.2

Question 26

What are the two Methods of Frozen Section Preparation?

Answer 26

Cold Knife Procedure Cryostat Procedure/ Cold Microtome

Question 27

Optimum condition for sectioning using cold Knife method Knife: Tissue: Environment:

Answer 27

Knife: -40°C to -60°C Tissue: -5°C to -10°C Environment: 0°C to -10°C

Question 28

Optimum condition for sectioning using cold Knife method Knife: Tissue: Environment:

Answer 28

Knife: -40°C to -60°C Tissue: -5°C to -10°C Environment: 0°C to -10°C