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MLT > Exam3 > Flashcards

Exam3 Flashcards

0
Q

A solid cone of light delivered to specimen by condenser and gathered by the objective

A

Aperture

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1
Q

What removes dried oil from the lens the best?

A

Xylene

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2
Q

Ability of scope to reveal or separate fine detail

A

Resolution

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3
Q

Distance from center of objective to top of specimen also called focal length

A

Working distance

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4
Q

Imperfect refraction or focalization of a lens inability to bring light rays to a single focus

A

Aberration

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5
Q

Reflects the beam of light upward

A

Mirror

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6
Q

Directs and focuses the light from the light source up to the specimen

A

Condenser

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7
Q

Controls the amount of light

A

Iris diaphragm

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8
Q

How do u calculate the total magnification of a specimen on a microscope

A

Ocular x objective

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9
Q

What is magnification of most oculars

A

10x

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10
Q

Three most common objectives and what are they otherwise known for

A

10 low power
40-45 high dry
100 oil immersion

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11
Q

The visible area through an infocused lens

A

Field of view

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12
Q

Name 6 types of microscope

A 
Brightfield
Darkfield
Flourscent
Phase contrast
Polarized
Electron
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13
Q

Microscope use daylight or light bulb with compound lens system

A

Brightfield

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14
Q

Microscope uses Special condenser makes background appear dark condenser cause light waves pass specimen instead of through it

A

Darkfield

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15
Q

Microscope uv light source and special filter for shorter wave lengths

A

Fluorescent

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16
Q

Microscope special diaphragm into or below condenser to view u stained structures

A

Phase contrast

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17
Q

Microscope-use special filter takes ordinary light waves and allows only light waves to pass through and reach specimen illuminate objects that can rotate light N to S

A

Polarized

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18
Q

Microscope- substitution of electron beams for light rays to achieve the resolution

A

Electron

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19
Q

Information on potentially hazardous chemicals and how to work safely with them

A

MSDS

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20
Q 
Name Colors of diamond hazard symbol and information rated 0-4
1 fire
2 health
3 Reactivity
4 Specific hazard
A

1 red
2 blue
3 yellow
4 white

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21
Q

Treating all specimens as potentially infected is called

A

Standard precautions

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22
Q

What class of bioterrorism agent is anthrax botulism small pox plaque

A

Class a

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23
Q

What class of bioterrorism agents is bacterial c perfringens E. coli 0157:H7 salmonella ricin toxin

A

Class b

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24
Q

What class of bioterrorism agents is hiv aids hantavirus

A

Class c

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25
Q

Single most effective way to reduce contaminating yourself

A

Washing hands

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26
Q

How long is HBV stable in blood products at 25 c?

A

7 days

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27
Q

Where should contaminated materials be disposed?

A

Biohazard container

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28
Q

What should employee do if they were to get caustic chemicals on skin?

A

Wash 5 min seek medical attention

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29
Q

Explain what employee should do if they get caustic chemicals in their eyes

A

Wash 15 minutes seek medical attention

30
Q

Name 5 safety features labs should have

A 
Fire blanket
First aid kit
Spill kit 
eyewash
PPE
Shower
Fire extinguisher
31
Q

Means contents of pipettes should be rinsed out with solution it is being added to

A

T.C. To contain

32
Q

Means -contents of pipettes should be blown out

A

T.D. To deliver

33
Q

Glassware resists normal cleaning should be cleaned with? (2)

A

Solution of potassium and concentrated sulfuric acid

34
Q

2 brands of heat resistant glassware

A

Pyrex

Kimax

35
Q

Pipettes -Bulb in center calibrated to deliver fixed volume of liquid used when great accuracy is needed

A

Volumetric pipettes

36
Q

Pipettes graduated pipettes delivered full volume mark to mark

A

Serological pipettes

37
Q

Pipettes with bulb close to deliver tip used for viscous liquids

A

Ostwald-Folin pipettes

38
Q

Pipettes deliver full volume mark to tip

A

Graduated pipettes

39
Q

What does an etched ring near the mouth of pipettes mean

A

Blow out

40
Q

Have flat bottoms sloping sides to neck no critical mixing

A

Erlenmeyer flask

41
Q

Wide mouth straight sides used for estimating liquid amounts used for mixing heating and storage

A

Beakers

42
Q

Cylidgerical glass or plastic several calibrated marking from 5ml to 2 liters not for precise volume but reagent water

A

Graduated cylinders

43
Q

Flask Calibrated and used for more precise measurements

A

Volumetric flask

44
Q

What is low actinic glass used for?

A

Light sensitive solutions

45
Q

Most common material used for plastic ware in lab

A

Polyethylene

46
Q

Reference material from which 99.95% of chemical can be retrieved

A

Primary standard

47
Q

Reference material in which analyte concentration has been ascertained by reference and is most commonly used in labs

A

Secondary standard

48
Q

high degree of purity most commonly used in clinical labs

A

A.R. Analytical reagent

49
Q

Limit of impurities tolerated suitable for general application

A

C.P. Chemically pure

50
Q

Less pure than c.p but reactant meet specification used in medications (2)

A

N.F. National formulary

USP untied states pharmacopeia

51
Q

Boiled steamed cooled vapors collected mineral remain beyond contain dissolved gases and organic solvents

A

Distilled water

52
Q

Passed through a column of charged resin particles particles combine with ions present in water to remove them removes substance containing electrical charges

A

Deionized water

53
Q

Distiller water re distiller higher degree of purity

A

Double distilled water

54
Q

Ideal for reconstituting chemistry or coag products preparing standards or rinsing electrodes prepared using reverse osmosis mixed bed deionzaton activated carbon filtration and final filtration essential free from organic and inorganic particulate and soluble contaminants

A

Reagent grade water

55
Q

Explain proper storage of reagents and chemicals(3)

A

1Cool dry area
2properly labeled and within easy reach
3 proper temperature

56
Q

Should reagents ever be stored unlabeled?

A

No

57
Q

Name 5 things a reagent label must contain

A 
1date made
2 name
3 person who made
4 cautions
5 expiration date
58
Q

What is the expiration date of a reagent if it is not specified?

A

1 month

59
Q

Steps u take once u have transferred chemical from weigh boat into receiving vessel to ensure accurately prepared solution (2)

A

1Rinse weight boat

2 and funnel with your solvent

60
Q

Centrifuge cups positioned at fixed angle

A

Fixed angle rotor centrifuge

61
Q

Centrifuge cups begin vertical but swing out hozoiontal while spinning

A

Horizontal rotor centrifuge

62
Q

Centrifuge that can reach high speed up 100,000 rpm used to separate specimen that usually take hours or days to separate

A

Ultracentrifuge

63
Q

Centrifuge used in hemotology to centrifuge small amount of blood used for hemocrit determination

A

Microhematocrit centrifuge

64
Q

Centrifuge table or floor model can spin large amounts of blood;

A

Refrigerated blood bank centrifuge

65
Q

Centrifuge small centrifuge with low and high speed

A

Serofuge

66
Q

Used to wash RBC’s

A

Cell washers

67
Q

Centrifuge makes slides in monolayer and used for body fluid analysis

A

Cytospin centrifuge

68
Q

List 4 precautions when operating a centrifuge

A

1 glass breakage and spillage must be cleaned out immediately
2 never stop with hands
3 keep caps on vials or para film them
4 do not open lid till completely stopped

69
Q

Define the accuracy of a trip balance vs analytical balance

A

Trip 0.1 gram

Analytical 0.1mg

70
Q

Describe the proper placement of a analytical balance (3)

A

1Flat surface
2Away from heat
3and movement

71
Q

What function does glass enclosure and balancing screws have on a analytical balance?

A

Glass protects from air current

Balancing screw level balance

72
Q

What is the proper way to handle objects that are to be weighed?

A

Tongs

MLT (2 decks)

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