Exam3

Question 0

A solid cone of light delivered to specimen by condenser and gathered by the objective

Answer 0

Aperture

Question 1

What removes dried oil from the lens the best?

Answer 1

Xylene

Question 2

Ability of scope to reveal or separate fine detail

Answer 2

Resolution

Question 3

Distance from center of objective to top of specimen also called focal length

Answer 3

Working distance

Question 4

Imperfect refraction or focalization of a lens inability to bring light rays to a single focus

Answer 4

Aberration

Question 5

Reflects the beam of light upward

Answer 5

Mirror

Question 6

Directs and focuses the light from the light source up to the specimen

Answer 6

Condenser

Question 7

Controls the amount of light

Answer 7

Iris diaphragm

Question 8

How do u calculate the total magnification of a specimen on a microscope

Answer 8

Ocular x objective

Question 9

What is magnification of most oculars

Answer 9

10x

Question 10

Three most common objectives and what are they otherwise known for

Answer 10

10 low power
40-45 high dry
100 oil immersion

Question 11

The visible area through an infocused lens

Answer 11

Field of view

Question 12

Name 6 types of microscope

Answer 12

Brightfield
Darkfield
Flourscent
Phase contrast
Polarized
Electron

Question 13

Microscope use daylight or light bulb with compound lens system

Answer 13

Brightfield

Question 14

Microscope uses Special condenser makes background appear dark condenser cause light waves pass specimen instead of through it

Answer 14

Darkfield

Question 15

Microscope uv light source and special filter for shorter wave lengths

Answer 15

Fluorescent

Question 16

Microscope special diaphragm into or below condenser to view u stained structures

Answer 16

Phase contrast

Question 17

Microscope-use special filter takes ordinary light waves and allows only light waves to pass through and reach specimen illuminate objects that can rotate light N to S

Answer 17

Polarized

Question 18

Microscope- substitution of electron beams for light rays to achieve the resolution

Answer 18

Electron

Question 19

Information on potentially hazardous chemicals and how to work safely with them

Answer 19

MSDS

Question 20

Name Colors of diamond hazard symbol and information rated 0-4
1 fire
2 health
3 Reactivity
4 Specific hazard

Answer 20

1 red
2 blue
3 yellow
4 white

Question 21

Treating all specimens as potentially infected is called

Answer 21

Standard precautions

Question 22

What class of bioterrorism agent is anthrax botulism small pox plaque

Answer 22

Class a

Question 23

What class of bioterrorism agents is bacterial c perfringens E. coli 0157:H7 salmonella ricin toxin

Answer 23

Class b

Question 24

What class of bioterrorism agents is hiv aids hantavirus

Answer 24

Class c

Question 25

Single most effective way to reduce contaminating yourself

Answer 25

Washing hands

Question 26

How long is HBV stable in blood products at 25 c?

Answer 26

7 days

Question 27

Where should contaminated materials be disposed?

Answer 27

Biohazard container

Question 28

What should employee do if they were to get caustic chemicals on skin?

Answer 28

Wash 5 min seek medical attention

Question 29

Explain what employee should do if they get caustic chemicals in their eyes

Answer 29

Wash 15 minutes seek medical attention

Question 30

Name 5 safety features labs should have

Answer 30

``` Fire blanket First aid kit Spill kit eyewash PPE Shower Fire extinguisher ```

Question 31

Means contents of pipettes should be rinsed out with solution it is being added to

Answer 31

T.C. To contain

Question 32

Means -contents of pipettes should be blown out

Answer 32

T.D. To deliver

Question 33

Glassware resists normal cleaning should be cleaned with? (2)

Answer 33

Solution of potassium and concentrated sulfuric acid

Question 34

2 brands of heat resistant glassware

Answer 34

Pyrex | Kimax

Question 35

Pipettes -Bulb in center calibrated to deliver fixed volume of liquid used when great accuracy is needed

Answer 35

Volumetric pipettes

Question 36

Pipettes graduated pipettes delivered full volume mark to mark

Answer 36

Serological pipettes

Question 37

Pipettes with bulb close to deliver tip used for viscous liquids

Answer 37

Ostwald-Folin pipettes

Question 38

Pipettes deliver full volume mark to tip

Answer 38

Graduated pipettes

Question 39

What does an etched ring near the mouth of pipettes mean

Answer 39

Blow out

Question 40

Have flat bottoms sloping sides to neck no critical mixing

Answer 40

Erlenmeyer flask

Question 41

Wide mouth straight sides used for estimating liquid amounts used for mixing heating and storage

Answer 41

Beakers

Question 42

Cylidgerical glass or plastic several calibrated marking from 5ml to 2 liters not for precise volume but reagent water

Answer 42

Graduated cylinders

Question 43

Flask Calibrated and used for more precise measurements

Answer 43

Volumetric flask

Question 44

What is low actinic glass used for?

Answer 44

Light sensitive solutions

Question 45

Most common material used for plastic ware in lab

Answer 45

Polyethylene

Question 46

Reference material from which 99.95% of chemical can be retrieved

Answer 46

Primary standard

Question 47

Reference material in which analyte concentration has been ascertained by reference and is most commonly used in labs

Answer 47

Secondary standard

Question 48

high degree of purity most commonly used in clinical labs

Answer 48

A.R. Analytical reagent

Question 49

Limit of impurities tolerated suitable for general application

Answer 49

C.P. Chemically pure

Question 50

Less pure than c.p but reactant meet specification used in medications (2)

Answer 50

N.F. National formulary | USP untied states pharmacopeia

Question 51

Boiled steamed cooled vapors collected mineral remain beyond contain dissolved gases and organic solvents

Answer 51

Distilled water

Question 52

Passed through a column of charged resin particles particles combine with ions present in water to remove them removes substance containing electrical charges

Answer 52

Deionized water

Question 53

Distiller water re distiller higher degree of purity

Answer 53

Double distilled water

Question 54

Ideal for reconstituting chemistry or coag products preparing standards or rinsing electrodes prepared using reverse osmosis mixed bed deionzaton activated carbon filtration and final filtration essential free from organic and inorganic particulate and soluble contaminants

Answer 54

Reagent grade water

Question 55

Explain proper storage of reagents and chemicals(3)

Answer 55

1Cool dry area 2properly labeled and within easy reach 3 proper temperature

Question 56

Should reagents ever be stored unlabeled?

Answer 56

No

Question 57

Name 5 things a reagent label must contain

Answer 57

``` 1date made 2 name 3 person who made 4 cautions 5 expiration date ```

Question 58

What is the expiration date of a reagent if it is not specified?

Answer 58

1 month

Question 59

Steps u take once u have transferred chemical from weigh boat into receiving vessel to ensure accurately prepared solution (2)

Answer 59

1Rinse weight boat | 2 and funnel with your solvent

Question 60

Centrifuge cups positioned at fixed angle

Answer 60

Fixed angle rotor centrifuge

Question 61

Centrifuge cups begin vertical but swing out hozoiontal while spinning

Answer 61

Horizontal rotor centrifuge

Question 62

Centrifuge that can reach high speed up 100,000 rpm used to separate specimen that usually take hours or days to separate

Answer 62

Ultracentrifuge

Question 63

Centrifuge used in hemotology to centrifuge small amount of blood used for hemocrit determination

Answer 63

Microhematocrit centrifuge

Question 64

Centrifuge table or floor model can spin large amounts of blood;

Answer 64

Refrigerated blood bank centrifuge

Question 65

Centrifuge small centrifuge with low and high speed

Answer 65

Serofuge

Question 66

Used to wash RBC's

Answer 66

Cell washers

Question 67

Centrifuge makes slides in monolayer and used for body fluid analysis

Answer 67

Cytospin centrifuge

Question 68

List 4 precautions when operating a centrifuge

Answer 68

1 glass breakage and spillage must be cleaned out immediately 2 never stop with hands 3 keep caps on vials or para film them 4 do not open lid till completely stopped

Question 69

Define the accuracy of a trip balance vs analytical balance

Answer 69

Trip 0.1 gram | Analytical 0.1mg

Question 70

Describe the proper placement of a analytical balance (3)

Answer 70

1Flat surface 2Away from heat 3and movement

Question 71

What function does glass enclosure and balancing screws have on a analytical balance?

Answer 71

Glass protects from air current | Balancing screw level balance

Question 72

What is the proper way to handle objects that are to be weighed?

Answer 72

Tongs