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Discuss the evidence for the theory that mitochondria may have evolved from free-living prokaryotes by endosymbiosis. [3 marks]
a. «double» membrane may have formed when engulfed
d. they have «70s» ribosomes AND can manufacture «their own» proteins
b. replicate by binary fission like free-living prokaryotes
Identify the stage of mitosis labelled X in the image, giving a reason. [1 mark]
telophase because the chromosomes/chromatids have reached the poles
The structure of prokaryotic cells has been investigated using electron microscopy. Draw a labelled diagram to show prokaryotic cell structure. [4 marks]
a. cell wall — a uniformly thick wall ✔
b. pili — hair-like structures connected to cell wall
d. «70S» ribosomes — drawn as small discrete dots not circles ✔
e. naked DNA/nucleoid — region with DNA not enclosed in membrane ✔
Discuss the cell theory and its limitations. [7 marks]
Cell Theory:
a. cell theory is the accepted explanation of life ✔
b. organisms are composed of «one or more» cells ✔
c. cells are the basic/fundamental/smallest units of life ✔
d. cells can only come from pre-existing cells ✔
e. spontaneous generation of life has been disproven ✔
Limitations:
f. striated muscle cells contain many nuclei «while most eukaryotic cells have one nucleus»
g. giant algae have complex single cell structure
Draw a labelled diagram to show the fluid mosaic model of the plasma membrane. [4 marks]
a. two correctly oriented layers of phospholipids/phospholipid bilayer shown with heads facing in opposite directions
b. phospholipids shown with two parts labelled hydrophilic/phosphate head AND hydrophobic/hydrocarbon tail
c. protein (any) shown as a globular structure embedded in one/both layers of phospholipid
d. peripheral protein, shown as globular structure at the surface of the membrane AND integral protein shown as embedded globular structures
Unicellular and multicellular organisms share the same functions of life. Outline four functions of life. [4 marks]
b. metabolism: conversion of organic molecules/chemical reactions in an organism
c. growth: increase in size/mass/number of cells within an organism
d. response/irritability/sensitivity: reactions/responsiveness to stimuli/factors
e. homeostasis: regulating/maintaining constant/stable interior environment
State the name of the structures labelled I and II. [1 mark]
I. cell wall
II. nucleus/chromatin
Outline the function of the structure labelled III. [2 marks]
a. necessary for photosynthesis/converts light energy into chemical energy
b. contains chlorophyll to absorb light
Describe how Pasteur’s experiments provided convincing evidence to falsify the concept of spontaneous generation [3 marks]
a. spontaneous generation is life appearing from nothing / from non-living / cells only come from pre-existing cells/life
b. broth/culture medium (for bacteria) (used/placed) in flasks
c. broth boiled/sterilized «in some flasks» to kill microbes
State the function of life in Paramecium that is carried out by: cilia. [1mark]
movement/locomotion
State the function of life in Paramecium that is carried out by: the contractile vacuole.
[1mark]
homeostasis
Discuss the advantages and disadvantages of the use of adult stem cells. [3 marks]
Advantages:
a. «adult stem cells» can divide «endlessly» / can differentiate
b. «adult stem cells» can be used to repair/regenerate «tissues»
Disadvantages:
i. difficult to obtain/collect/find in adult body/very few available
(i) State the process that divides one nucleus into two genetically identical nuclei.
(ii) Explain how the cell cycle is controlled.
[5 marks]
(i) mitosis
(ii)
a. cell cycle is a sequence of stages / cell cycle is G1, S, G2 and mitosis
b. (control of the cell cycle) by cyclins/cyclin
c. levels of cyclins rise (and fall)/fluctuate during the cell cycle/surge at different times/have to reach a certain concentration
d. conditions inside as well as outside the cell affect regulation
Outline the stages of the cell cycle.
[5 marks]
a. interphase is the longest phase;
b. interphase includes G1, S and G2;
c. in G1 and G2 /G phases, cell performs normal functions/protein synthesis/cell grows/organelles are replicated;
d. S/synthesis phase when the DNA replicates;
e. mitosis is when nucleus/genetic material divides;
Cell membranes separate aqueous environments in cells. Explain how the properties of phospholipids help to maintain the structure of cell
membranes.
[8 marks]
a. (labelled) phospholipid consisting of head and two tails;
b. head is glycerol and phosphate;
c. tails are fatty acid chains;
d. head hydrophilic and tails hydrophobic;
e. hydrophilic molecules/heads attracted to/soluble in
water;
f. hydrophobic molecules/tails not attracted to water but attracted to each other;
g. (properties of phospholipids leads to) formation of double layer in water;
h. stability in double layer because heads on outer edge are attracted to water
Milk contains lactose which some people can digest but some cannot. Explain the production of lactose-free milk.
[3 marks]
a. lactase added to milk / lactase immobilised;
b. lactose hydrolysed/broken down into glucose and galactose;
c. for people who are lactose intolerant/lack lactase;
Cellulose is the most abundant organic polymer on Earth. Describe the structure of cellulose. [3 marks]
a. unbranched/straight chain of glucose molecules
b. formed of beta glucose;
c. formed by condensation reactions/glycosidic bonds
State two structural features that differ between RNA and DNA. [2 marks]
a. number of strands
b. base composition
Water has important solvent properties. Explain these properties using an example to illustrate your answer.
[3 marks]
a. water molecule is polar
c. water forms hydrogen bonds with polar substances
d. positive/hydrogen side/pole of water attracted to negative ions
Compare and contrast cis-fatty acids and trans-fatty acids.
[2 marks]
a. both are unsaturated fatty acids
b. in cis-fatty acids the two H atoms are on the same side while in trans-fatty acids they are on opposite sides
Outline the stages in the production of mRNA by transcription.
[4 marks]
a. DNA is unwound/strands are separated «by RNA polymerase» ✔
b. new nucleotides attached to template strand «by RNA polymerase» ✔
d. mRNA detaches from template ✔
e. DNA rewinds ✔
Outline how translation depends on complementary base pairing. [3 marks]
a. translation converts a sequence of mRNA nucleotides to a sequence of amino acids
b. «triplets of» nucleotides «activated» tRNAs pair with complementary «triplets of» nucleotides on mRNA
c. base pairing occurs when adenine pairs with uracil and guanine pairs with cytosine
Describe the polymerase chain reaction (PCR), including the role of Taq DNA polymerase. [4 marks]
a. PCR is process by which a small sample of DNA can be amplified/copied many times
b. PCR involves repeated cycling through high and lower temperatures «to promote melting and annealing of DNA strands»
c. «mixture» is heated to high temperatures to break «hydrogen» bonds between strands of DNA/to separate the double-stranded DNA
d. Taq DNA polymerase can withstand high temperatures without denaturing
Draw the structure of a section of DNA showing all possible bases.
[4 marks]
k
Outline the structural and genetic characteristics of eukaryotic chromosomes. [4 marks]
a. linear/not circular DNA molecule
b. associated with histone proteins/nucleosomes
c. centromere joins sister chromatids «after DNA
replication»
d. telomeres at the end «of the chromosome/chromatid»