Exam Practice Qs Flashcards

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1
Q

Microscopy

Two structures that are separated by a distance less than half that of the radiation wavelength used in the microscope cannot be seen as separate objects.

If the average wavelength of white light (visible light) is 550 nm (nanometres), what is the resolution limit of a light microscope using this light source?

A

= 550/2
= 225nm

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