Exam Flashcards
Explain Universal Precautions as it applies to phlebotomy
Blood and certain body fluids of all individuals are considered potentially infectious.
Explain Standard Precautions as it applies to phlebotomy
Precautions to use in caring for all patients regardless of diagnosis or presumed infection status. Standard precautions apply to all body fluids. Wear proper PPE
Explain Transmission based precautions as it applies to phlebotomy
Precautions to use for patients either suspected or known to be infected with certain pathogens transmitted by airborne, droplet, or contact routes
What are 5 steps that can be taken to reduce the risk of exposure to bloodborne pathogens?
1) Wash hands when changing gloves and between patients.
2) Wear gloves during all blood collections.
3) Wear protective covering when clothing is likely to be soiled.
4) Wear mask or eye protection.
5) Properly dispose of needles / syringes / sharps.
What are typical precautionary measures and actions that are taken in the event of an accidental needle puncture or body substance exposure?
Immediately cleanse exposed area.
Report incident to supervisor immediately.
Healthcare worker is tested for HIV, HBV and hepatitis C if consent is given.
If source patient is known, consent must be obtained and baseline HIV, HBV and hepatitis C tests are performed.
If source patient refuses testing, worker has a right to prophylaxis treatment.
Follow-up testing
Describe Protective (Reverse) Isolation. When is it utilized? What is the phlebotomist’s responsibility to a patient in protective isolation?
Protective isolation is designed to protect the patient from the healthcare worker.
Patients who are immunosuppressed or transplant patients are often in protective isolation.
PPE must be worn before entering the patient’s room. All phlebotomy supplies need to remain outside of the patients room or cleaned before be brought in.
List two instances when a syringe is more applicable than the evacuated® tube method for blood draw?
Drawing from a vein other than in the anticubital area.
Difficult-to-draw patient who have fragile, thin, or “rolling” veins.
List the anticubital veins in order of choice for routine venipuncture:
a. Median cubital vein
b. Cephalic vein
c. Basilic vein
What step should be done prior to beginning a specimen collection utilizing a syringe?
Pull back plunger to remove air lock.
State the relationship between bore size and the gauge of the needle.
The larger the gauge the smaller the bore. A larger gauge needle would be used on a narrow vein.
How does EDTA prevent coagulation?
Binds/chelates calcium
How does Potassium Oxalate prevent coagulation?
Precipitates calcium
How does Citrate prevent coagulation?
Binds/chelates calcium
How does Fluoride prevent coagulation?
Inhibits glycolysis
How does Heparin prevent coagulation?
Inhibits thrombin
What anticoagulant is in the following?
Light blue
Gray
Green
Light blue - Sodium citrate 3.2%
Gray - Lithium iodoacetate and lithium heparin
Green - Sodium heparin or Lithium heparin
What anticoagulant is in the following?
Lavender (purple)
Red
Red/black
Lavender - EDTA
Red - No anticoagulant
Red/black - gel/clot activator. Sodium heparin
What is the purpose of adding a silicon coating to a collection tube?
Fills the microscopically rough surfaces of glass so that cells don’t adhere to the sides of the tube.
Reduces the chance for hemolysis.
What is the purpose of adding silica particles to a collection tube?
Acts as a clot activator, initiates clotting process
What is the purpose of adding thixotropic gel to a collection tube?
Inert gel that undergoes change in viscosity during centrifugation.
Acts as an interface between cells, serum or plasma
In what order should blood tubes be drawn for evacuated tube?
Stop Light Red Stay Put, Green Light Go
Sterile (Blood culture), light blue, red, SST and PST(gold), green, lavender, grey
What role does the tourniquet play in specimen collection? What effects are there if the tourniquet remains on to long?
Used to access and find a good vein
Leaving it on to long can cause hemoconcentration/hematoma.
Describe different types of lancets used in capillary specimen collection based on area of collection and depth of puncture.
Neonatal to 12 months of age: heelsticks, < 2.0 mm
Shallow incision: for toddlers, children, = 1.8 mm
Normal incision: point of care tests. = 2.4 mm
Deeper incision: = 3.0 mm
Explain social, clerical, and technical skills.
Social - skills involving the patient
Clerical - skills with data entry and record keeping
Technical - skills drawing the patient