Exam 4

Question 1

The figure above illustrates the difficulty of sequencing [What?]:

Answer 1

A repeat region [In this case, a dinucleotide repeat]

Question 2

What about the figure above illustrates part of a genotyping procedure?

Answer 2

The molecule on the left is a ddNTP. This is used to terminate extension during a cycle sequencing reaction.

Question 3

The peak spacing and shape problems at the left in the electropherograms above reflect the difficulty of adjusting for the effect of ______ for small DNA fragments.

Answer 3

Dye mobility

Question 4

Using the formula including theta (In contrast to using the Hardy Weinberg Formula) will have this effect on the random match probability.

Answer 4

RMP will become greater.

Question 5

What is a Pseudogene?

Answer 5

A locus with a sequence mostly characteristic of a gene but not close enough to be a functional gene.

Question 6

What is the term used for a sequence electropherogram peak quality score?

Answer 6

Phred