Exam 4 Flashcards
A fertilized egg will form _______ that cell will divide many times and as it divides it will differentiate into different tissues.
zygote
Our body after cell division and differentiation has about ____
200 different cell types
What is Stem cells?
Stem cells stay behind they sit in the surrounding tissue that divides and differentiates and they stop dividing and sit undifferentiated unspecialized.
_________can give rise to specialized cells once they are called to action.
Stem cells
Scientist discover that some stem cells if kept in the lab can_______
cell divide and while others cannot, some can also differentiate into whole array of cells, and some cannot.
What is High Genetic potential?
If I have a stem cell that can divide and differentiate with success for years, then I say this cell has high genetic potential
Genetic potential of stem cells decreases with _______
our age
What are the two types of stem cells?
Embryonic stem cells and adult stem cells
What’s the difference between Embryonic stem cells and adult stem cells when it comes to differentiate.
Embryonic stem cells: can still differentiate and adult stem cells: can no longer differentiation
Embryonic stem cells are called__________
pluripotent
What can Embryonic stem cells do?
they can differentiate into pretty much any type of the cell in the body
What can Adult stem cells do?
they can differentiate into a limited number of cells there
Ault stem cells are called_________
multipotent
What’s the process of hierarchy of stem cells?
First we start with the totipotent which makes the entire organism, then we have pluripotent which is an embryonic stem cell then they turn into adult stem cells to
Stem cell will always get the _______ DNA
maternal
Where are adult stem cells found?
most parts of the body, including brain, bone marrow, blood vessels, skin, teeth and heart
where do we collect Embryonic stem cells?
Can be collected at the blastocyst where we have 100 cells
Embryonic stem cells can be kept alive____.
can be kept alive indefinitely
What is Stem cell line?
A replicating set of stem cells from a single blastocyst
Embryonic stem cell are capable of ________into almost any type of cell
differentiating
Why are people against on getting Embryonic stem cells?
we destroy the embryo
Cancers cells have the same characteristics to ____________able to _______
Embryonic stem cells, divided fast
Use for repair
Adult stem cells
Caple of differentiating into cell type of the tissue in which they reside
Adult stem cells
Plasticity and survival is limited
Adult stem cells
Very small number of stem cells in each tissue
Adult stem cells
Which of the following statements correctly describes characteristics of adult cells and other cell isolated from the same tissue at the same time?
The two cell types have different patterns of DNA methylation
Adult cells reprogrammed by ________.
gene transfer
What does iPS stand for
Induced pluripotent stem cells
What is IPS?
skin cells were transformed into Es cells by using retroviruses to introduce extra cloned copies of four stem cells regulatory genes
Which of the following processes is used to convert adult cells into pluripotent stem cells (Ips).
A retrovirus is used to introduce four specific master regulatory genes
Making iPS cells from sick people provides a way to study the cells of those people
Including:
-Huntington’s disease
-Gaucher disease
-type I juvenile diabetes
What are mesenchymal stem cells?
multipotent stem cells found in bone marrow that are important for making and repairing skeletal tissues, such as cartilage, bone and the fat found in bone marrow
Stem cell Based Therapy for the treatment of _________
Burn
What is Therapeutic cloning?
using stem cells to cure someone like heat, cancer
Its cloning because they are the same genetically cells only with different gene expression
What is Reproductive cloning?
attempting to create a new organism with is genetically identical to its parent
The cloning of Dolly the sheep is an example of which of the following processes?
transfer of an adult cell’s nucleus into an enucleated sheep egg, followed by incubation in a surrogate
What are some reasons why Dolly the sheep die young.
- It could be due to the length of the telomeres as we age we lose telomeres because of DNA replication of the lagging strength
It could also be that the epigenome wasn’t wipe out
Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning, there is a wider divergence of opinion. Which of the following statement provides the best argument in support of therapeutic cloning?
cloning to produce embryonic stem cells may lead to great medical benefits for many
cancer is a genetic disease because _______________
it relates to our genetic material.
Cancer is not a genetic disease______
in many cases is not inherited
What happens in the (first mutation) ?
boost of energy happens and replace the nucleotide (first mutation) in that one cell and that cell did not die, can reproduce cellularly by mitosis
What happens in the second mutation?
(Second mutation) a clone from the first mutation and each of them is carrying that first mutation. The cell is still doing ok.
What happens in the third mutation?
(Third mutation) a patch of cells that now carry two mutations
What can Healthy cell
do?
-reproduce when it is told to divide and it obeys signals that are coming from two sides, opposite sides that are generated by proteins and proteins are coded by genes.
-cell goes through cell cycle
-cell division requires collaboration of multiple proteins to either promote cell division or stop cell division
What is proto-oncogenes (good genes)?
Those genes that promote cell division
What is tumor suppressor genes (good genes)?
tells the cell wait a minute it’s not time to divide now
If everything goes ok the cell checks off _________ that are placed in the cell cycle
check points
How does the cell go through all the check points?
- If everything goes ok the cell checks off check points that are placed in the cell cycle
- The first set of proteins makes check one point. G1 check point is checking if the cell is good to divide, also if its time to divide
- When the cell passes the check point it will replicate its DNA and it needs repair the DNA replication. It will check point G2. When the cell is given the green light to proceed
- The cell will undergo mitosis. The nuclear material will separate into two daughter cells at the telophase when the mitotic check point happens and check if chromosomes separated, chromatid separated properly
- Cytokinesis follows
Stage one cancer
when the tumor is still in one location and its malignant (stage one)
Stage two cancer
when it grows and starts to infect nearby tissue (stage two)
Stange three cancer
When it grows and gets into blood lymbh vessles and can travel through the body
Stange fourth cancer
when cancer cells re-establish themselves in another location
What are the check points?
G1 to check if the cell is good to divide, is it time to divide
-G2Check point two checking if the replication was ok
-M check point to see if chromatid separated properly
What is cell cycle control system?
The sequential events of the cell cycle are directed
What is checkpoints?
where the cell cycle stops until a go-ahead signal is received
Cancer cells may not need ______ to grow and divide:
-make their own ______
growth factors
cancer cells may convey a growth factor’s signal without the presence of _______
-they may have an abnormal _________
- the growth factor
2.cell cycle control system
What is Transformation?
A normal cell is converted to a cancerous
What is a benign tumor?
if abnormal cells remain at the original site
What is Malignant tumor?
invade surrounding tissues and can metastasize
What is Metastasize?
exporting cancer cells to other parts of the body, where they can form secondary tumors
What is Metastasis?
The ability of these cells to spread to other sites in the body
a tumor is form is when
cells may reproduce at the wrong time or place
What is HeLa?
cells are widely used around the world in cancer labs
Genes that regulate cell growth and programmed cell death
- Oncogenes-mutated proto-oncogenes
- Tumor suppressor gene (TSGs)
Mutated genes that suppress cell divisions
- Tumor suppressor gene (TSGs)
- DNA repair genes-mutated genes of DNA repair (good genes)
which of the following describe the role typical proto-oncogenes have when they are expressed in cells that are not cancerous?
They stimulate normal cell growth and division
Which of the following types of mutation would convert a proto-oncogene into an oncogene?
a mutation that greatly increases the amount of the proto-oncogene protein
are the receivers of the signals from the outside that would stimulate growth by stimulating cell division (grow factors)
What is Ras gene?
This is the gene that can be mutated and cause defective that would promote constant pushing of the cell to divide.
rase is for ______
oncogenes
Oncogenes are usually ___________
dominant
What is p53?
is for mutated tumor suppressor genes (cancers reflect environmental insults)
tumor suppressor gene are usually _____
recessive-acting mutation
What is which of the following describes the normal function of the P53 gene product?
It inhibits the cell cycle
What is Retinoblastoma?
this cancer occurs in children. Cancer of the eye
What is DNA repair gene
DNA repair gene
wont pass to generation
if the first mutation happens in the somatic tissue the cells will reproduce clonally, will have a patch of cancer cells
What is Polypolip?
cancer of the colon
wont pass to generation
if the first mutation happens in the somatic tissue the cells will reproduce clonally, will have a patch of cancer cells
its passed to the next generation
If the first mutation happens in the reproductive germ line. The person will have every mutation cancer cell and will be born with a predisposition to cancer
the somatic non inherited cancer develops after ______
age 50
familial cancer have an earlier onset. On average_____________ years old
10
What are Skin cancer types?
basal skin (should be removed)
-squamous cell (goes a little bit deeper)
-Melanoma (grows fast)
What is ABCDE rule
The halves wont match
B is not smooth
C different color
D for diameter
E for evolving
Taxol is an anticancer drug extracted from pacific yew tree. In animal cells. Taxol prevents microtube depolymerization, which interferes with which of the following processes?
Chromosome sepraration
What are early Detection?
PET: tumor image
Genetic testing: identify mutated genes
Screening large numbers of people: Enzyme tests markers for cancer
How does metastasis work?
- Cancer cells break away from their original tissue.
- The metastasizing cells become attached to the wall of a blood vessel or lymph vessel. They secrete digestive enzymes to create an opening. Then they cross the wall at the breach.
- Cancer cells creep or tumble along inside the bloodstream the same way they got in. They start new tumors in new tissues.
Genes that promote cancer for proto-oncogene
Oncogenes
Genes that promote cancer for tumor suppressor gene
Muted tumor suppressor gene
What is the Function of the membrane
is the boundary that separates living cell form often nonliving surroundings of from extracellular space. That separate cell from its environment
membrane creates that boundary the border is has to allow for the cell to _____________and _________ of the multicellular organism or_____
- uptake things that cell needs
2.release things that cell either produce for the benefit - the waist
The membrane has to be selectively permeable, allowing some substances to _______and some substances to be ____________
- cross
2.blocked from leaving or coming in
What is Transmembrane proteins?
protein is inside the cell, the other side of the protein is outside of the cell
What is Selective permeability?
allowing some substances to cross it more easily than others
What is Fluid mosaic model?
states that a membrane is a fluid structure with a mosaic of various proteins embedded in it
What are Phospholipids?
are the most abundant lipid in the plasma membrane
What is amphipathic molecules?
Are phospholipids containing hydrophobic and hydrophilic regions
Phospholipid, two ________ and a phosphate group are attached to ______
- fatty acids
- glycerol
the two fatty acid tails are _______, but the phosphate group and its attachments from a __________
1.hydrophobic
2. hydrophilic head
__________ are the major component of all cell membranes
phospholipids
Fatty acids are __________which means that they are afraid of water
hydrophobic
the head is _________, which means affinity for water
hydrophilic
Hydrophobic, fatty acid tails are inside the _____
bilayer
What is Phospholipid bilayer?
is constructed in a way that hydrophilic heads are facing outside.
Fluid unsaturated tails ____________
prevent packing
Viscous
saturated tails packed together
cholesterol within the animal cell membrane
Cholesterol reduces membrane fluidity at moderate temperatures, but at low temperatures hinders solidification.
Cholesterol moderates the fluidity of the membrane by hardening in___________
colder temperatures
-we have fatty acids that are_________
saturated and unsaturated
Saturated fatty acids have the tales straight because of ________-
hydrogenation
Unsaturated fatty acids have those__________ therefore they can __________________
- kinks
2.boost fluidity and permeability of the plasma membrane
you want to prevent rigid plasma membrane (you ______________ because ____________
- want to keep their fluidity
- that speaks to the plasticity)
If we have very rigid plasma membranes in the arteries, when we exercise and the blood flows faster, __________
the blood pressure increases the blood will be hitting the walls of the arteries and if the rigid are stiffed, they can break.
__________ keep the molecules from packing together
tails
Cholesterol reduces fluidity at moderate temperature by ______________________________It hinders solidification
reducing phospholipid movement; at low temp
The cholesterol promotes fluidity in _________________, reduces fluidity _________________-
1.warmer temperatures
2. in colder temperatures
Membranes must be ________to work properly
fluid
As temperatures cool, membranes switch from a fluid state to a _______
solid state
Membranes rich in _________ fatty acids are more fluid that those rich saturated fatty acids
unsaturated
Phospholipids are moving __________
around all the time
Small, nonpolar molecules can fit in the opening between___________
Phosphate group and glycerol heads
Small, uncharged but polar molecules (H2O is a polar molecule) can fit into the pores between the __________
phospholipid bilayer’s hydrophilic part
__________that do not fit in between their heads they will not be allowed to enter
Large molecules
_________polar molecules when they are large they will not be allowed to enter
Uncharged
_______________ like ions (they are not allowed)
Small but charged molecules
_________determine most of the membrane’s specific functions
Proteins
Proteins are embedded in the _______
fluid matrix
What is Peripheral proteins?
are bound to the surface of the membrane
What is Integral proteins?
penetrate the hydrophobic core integral proteins that span the membrane
What are the Six major functions of membrane proteins
1.Transport
2.Enzymatic function
3.Signal transduction
4.Cell- cell recognition
5.Intercellular joining
6.In animal cells we have extracellular matrix
What is Transport?
we can have proteins that are like hallways that are channels that allow for transport.) or (we can have proteins that are related to transport, but they need to open or close to allow things in or out)
What is Enzymatic function?
Enzymatic function (the proteins embedded in the plasma membrane can have a specific shape that matches the substrate and they have an enzymatic function)
What is Signal transduction
(cells need some kind of recognition) they can have plasmas membranes that are serving as the receptor to receive the signal. Those would be involved in signal transduction pathway and they are called receptors
What is Cell- cell recognition?
We also have proteins that are just like antennas sticking out and they are serving self-recognition part. That antenna can be recognized by another protein in the surrounding cells and that would be the way they communicate
What is Intercellular joining?
Proteins can also provide a kind of junctions that are joining cells together .
What is extracellular ?
In animal cells we have extracellular matrix where the proteins have other molecules attached to them some kind of fibers, collagen-based and so on. That provide that attachment of one cell to another
What is Cells recognize?
by binding to surface molecules, often containing carbohydrates, on the extracellular surface of the plasma membrane
- Membrane carbohydrates may be covalently bonded to_________ forming _________ or more commonly to _______forming ______
1.to lipids
2.glycolipids
3.proteins
4.glycoproteins
Carbohydrates on the external side of the plasma membrane vary among _________, _________ and even ______ in an individual
species, individuals,
cell types
Receptors are very important in __________, but also sometimes__________, because pathogens can get into the cell if the part of the pathogen body called _______________
- receiving signals
- blocking pathogens
3.antigen matches the shape of the receptor, like viruses
How is HIV blocked into cells?
HIV can infect a cell that has CCR5 on its surface, as in most people. HIV cannot infect a cell lacking CCR5 on its surface, as in resistant individuals.
The plasma membrane is found inside_______
the cell
In _____and in the _______ apparatus so the distribution of proteins and ________, ___________ and everything is determine when the membrane is built.
ER
Golgi
Proteins
lipids
Carbohydrates
the membrane is built by _______ and _________apparatus. The built membrane is then in the form of __________ traveling to the __________ and they fuses with the _________
ER
Golgi
vesicles
plasma membrane
plasma membrane
A cell must exchange materials with its ___________, a process controlled by the _________
surroundings
plasma membrane
Plasma membranes are selectively _________, regulating the cell’s __________
- permeable
2.molecular traffic
selective permeability refers to _______
Transport
What is diffusion?
If I have molecules that can pass through the membrane that can pass through the membrane, a passive transport based on chemistry and physics
The cell might need all the other ___________that are not allows to __________. Those molecules are transported mainly by _____________.
1.molecules
2. diffuse
3.active transport
Transport permeability of the ___________we have to differentiate between ________ and ____________.
- lipid bilayer
- passive transport
3.active transport
Passive transport is _________is the type of _________ when the molecules can pass through the membrane because of the ______ of the openings and also the ______.
- diffusion
2.passive transport
3.size
4.charges
What is Diffusion?
is the tendency of molecules to spread evenly in the available space. That refers to diffusion that can happen without a membrane being present.
What is an example of Diffusion?
I have perfumes and I spray them in the room even if somebody is on the other side of the room, after a while that person will smell the perfume
the movement of molecules is always down the ____________
concentration gradient
What is concentration gradient?
the difference in concentration of a substance from one area to another
No work must be done to move substances __________________because the molecules move by diffusion
1.down the concentration gradient
2. move by diffusion
the molecules from high concentration will move to the areas of _____________
lower concentration
when I talk about the movement of water we talk about diffusion of water called _______
osmosis
What is Osmosis?
is the diffusion of water across a selectively permeable membrane
Water diffuses across a membrane from the region of ___________ to the region of ___________
1.lower solute concentration
2.high solute concentration
solute will always move ___________
down the concentration gradient
from the lower solute concentration to the region of higher solute concentration. After a while I’ll have ___________
uneven levels of solution in my two arm dish
when I have a low concentration of sugar solute, I have ________water molecules free to ________. When I have high concentration of sugar , that sugar ___________. I have less water molecules available here.
- more
2.move
3.binds water
water will move from higher concentration of water to a __________ of water
lower concentration
Water also moves down the __________
concentration gradient
If I have concentrations of different molecules on one side higher than on the other side and if that molecule can move, it will move ______________________________________________
down the concentration gradient from high to low
the concentration of the solute as a reference point water will move from _________
one five molar solution
when I have a cell put into the environment that has the same concentration of chemicals water probably moves ________, but there is no net movement, this is an_____________
1.in and out
2. isotonic solution
What is Isotonic solution?
is a solution where the solute concentration is the same in the environment as it is in the solution
If I put a cell in the concentration higher than inside the cell water will move __________trying to________ the environment. The content of water inside the cell will______. The cell will ______.
1.out of the cell
2. dilute
3. decrease
4.shrivel
If I put this cell in the environment that has a lower concentration of solutes that inside the cell, then water will ___________
move into the cell.
-animals because they don’t have cell wall will uptake that water, uptake water (water will diffuse in, its not the will of the cell) to the point that the cell would _______, we call it ______
1.bust
2.lyses
If I have a plant cell, that cell will limit the diffusion of water, osmosis, by its cell wall. The plant will be normal, we say__________.
turgid
Isotonic solution for red blood cells is ________sodium chloride. Blood, our physiological fluids have _________ of sodium chloride
0.9%
low salt concentration the red blood cells will look like a _____, will have this _______ shape like a ____________
disc
donut without a hole