Exam 3 Practice Questions Flashcards
Where does glycolysis occur in a eukaryotic cell?
The cytoplasm
In glycolysis, what type of enzyme catalyzes the conversion of glucose-6-phosphate?
A kinase
Define Kinase
An enzyme involved in phosphoryl group transfer
In glycolysis, what type of enzyme catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate?
An isomerase
isomerase: Conversion between isomers
In glycolysis, how many ATP are required to convert 1 molecule of glucose to a molecule of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate?
2 ATP required
*** At this point, no ATP have been generated
In glycolysis, 1,3-biphosphoglycerate is converted to 3-phosphoglycerate by the enzyme phosphoglycerate kinase. What other reaction is coupled to this process?
ADP –> ATP
The net reaction of 1 glucose molecule that is converted to 2 molecules of pyruvate results in the production of how many molecules of NADH?
2
This reaction happens 2x per glucose
The net reaction of 1 glucose molecule that is converted to 2 molecules of pyruvate results i the NET production of how many molecules of ATP?
2 molecules of ATP
= -2 molecules of ATP expended
+4 molecules of ATP generated.
Total yield = 2
In the presence of 02, pyruvate will be converted predominantly to which molecule?
Acetyl CoA
What is the most direct purpose of the conversion of pyruvate to ethanol under anaerobic conditions in organisms that do fermentation?
To regenerate NAD+
Gluconeogenesis utilizes a lot of the same reactions as glycolysis but in reverse. There are however a few unique steps in the gluconeogenesis pathway. What intermediate is seen in the pathway of gluconeogenesis that is not seen in glycolosis?
Oxaloacetate
In the Cori cycle, _____ is converted to _____ in the liver
Lactate, glucose
In gluconeogensis, fructose 1,6-biphosphate is converted to fructose-6-phosphate by what class of enzyme?
phosphatase
When pyruvate is converted to acetyl CoA, what are the other products of the reaction?
NADH and CO2
Coenzyme A contains what organic functional group that binds acetyl groups?
Thiol
The condensation of acetyl CoA with oxaloacetate to form citrate is catalyze by what type of enzyme?
Synthase
The oxidation of isocitrate to form alpha-ketoglutarate is catalyze by what type of enzyme?
Dehydrogenase
How many CO2 are released for every acetyl CoA that is metabolized via the TCA cycle?
2
Approximately how many equivalents of ATP are generated (both directly and indirectly) per every acetyl CoA that is metabolized via the TCA cycle?
10 equivalents
A function of the glyoxylate cycle is:
to allow acetyl CoA to be converted into glucose
Complexes I, II, and III of the electron transport chain all contain what common cofactor?
Fe-S clusters
Which complexes in the electron transport chain contribute to the proton gradient that is established across the inner mitochondrial membrane?
Complexes I, III, And IV
NOT II
How many electrons are required to reduce 1 molecule of O2 to 2 molecules of H2O?
4
In the Boyer binding change mechanism for ATP synthase, proton passage through the protein complex results in ________
rotation of the Y subunit