Exam 3 lecture 15 study of rats and retina by Ochrietor

Question 1

Conclusions for the study is lg0 domain of basigin 1 binds to what

Answer 1

basigin 2

Question 2

basigin 1 does not induce what?

Answer 2

IL-6 expression in mouse monocytes

Question 3

did the results of the study support or contradict an earlier study of human cells?

Answer 3

the results contradicts

Question 4

the results of basigin-1 plays what kind of role n mouse and humans

Answer 4

possibly a different role in mouse than in humans.

Question 5

to test their hypothesis that lg0 domain of basigin-1 binds to basigin-2 what did they perform?

Answer 5

recombinant protein production.
-clone DNA
-transform bacterial cells with vector
-allow to transcribe
-extract protein
-analysis

Question 6

what were the 2 recombinant proteins of this study?

Answer 6

Lg0-6xHis
and
c-6xHis (control protein)

Question 7

what test did they do to test results?

Answer 7

they performed an ELISA analysis

Question 8

what was the independent variable and dependent variable?

Answer 8

I= the presences of the lg0 domain of basigin-1

D= The expression level of IL-6 in mouse monocytes

Question 9

main conclusion of this study

Answer 9

it does bind to basigin-2 but it doesn’t stimulate the IL-6 expression in mice monocytes

suggesting the role of inflammation could be species dependent