Exam 3 Flashcards
What is the basic structure of an amino acid?

What are the essential amino acids?
PVT TIM HALL
Phenylalanine
Tryptophan
Histidine
Valine
Isoleucine
(Arginine)
Threonine
Methionine
Leucine
Lysine
The other 11 are non-essential amino acids, what does that mean?
They can be synthesized by the body and we don’t have to provide it ourselves.
What is a complete protein?
Complete proteins contain all 9 essential amino acids in the required ratios.
What is an incomplete protein?
An incomplete protein is missing one or more essential amino acids.
Where do you find both complete and incomplete proteins in the diet?
Sources of complete proteins are from animal sources (meat, dairy, eggs).
Plant based proteins are typically incomplete proteins. Multiple sources of plant proteins should be combined to make a complete protein.
Protein recommendations for vegetarians and vegans
It’s recommended that they consume ~10% more protein than estimated requirements to ensure adequate essential amino acid intake and to account for the lower digestibility of plant proteins.
What are good protein sources for vegetarians and vegans?
- Black beans, chickpeas and other legumes
- Nuts and seeds
- Milk and dairy (for vegetarians)
- Quinoa
- Tempeh and Tofu
What is Nitrogen balance?
Nitrogen balance is a method to measure whole body protein balance.
Protein balance = synthesis – degradation
Nitrogen balance = nitrogen intake (protein) – nitrogen excretion (urea in urine)
What do the different protein balances mean?
When individuals are in protein balance (or nitrogen balance), there is no overall net gain or loss of protein.
Positive balance indicates protein accumulation, usually as a result of building muscle mass.
Negative balance indicates protein loss, which typically only occurs under sustained protein deficient diets and severe illness.
What is nitrogen balance during exercise like?
when beginning an exercise program, individuals will go into negative protein balance for the first 1-2 weeks. After this time period, nitrogen balance can be maintained without increasing protein intake.
Increases over the RDA will allow athletes to maintain positive nitrogen balance and build muscle mass.
Protein AMDR
The AMDR for protein is 10-35% of total caloric intake.
Protein RDA for sedentary adults
The RDA for protein for healthy, sedentary adults is 0.8 g/kg.
Protein recommendation for endurance athletes?
The recommendation for endurance athletes is 1.2-2.0 g/kg.
Protein recommendation for strength athletes?
The recommendation for strength athletes is 1.6-2.0 g/kg.
Do wesern diets supply enough protein?
Typically, western diets supply an abundance of high quality protein and deficiency is relatively uncommon. The general population has an intake substantially higher than the RDA.
Protein intake and aging
Aging adults also benefit from a higher protein intake than the RDA. High levels of protein stimulate muscle protein synthesis and help combat the muscle mass loss associated with aging.
Protein intake during weight loss correlation?
There is good evidence that a higher protein intake (on the order of a strength athlete’s recommendation) during weight loss will aid in retaining lean body mass.
How is protein digested? (Step by step)
Mechanical digestion begins in the mouth with chewing.
Chemical digestion begins in the stomach. HCl denatures proteins- breaking down the 3D structure. Pepsin breaks down proteins into smaller fragments (peptides).
In the small intestine, pancreatic enzymes called peptidases break down peptides to small strings of 1-3 amino acids.
Amino acids are absorbed into the intestinal cell and then the blood through transporters.
Amino acids are absorbed by cells and used for synthesizing new proteins, oxidation for energy or use in other metabolic intermediates. Some amino acids can be modified to form other (non-essential) amino acids when needed.
Amino acid transport into muscle cells is greatly enhanced by insulin.
What are some of the functions of protein other than maintaining and building muscle mass?
Enzymes used during chemical reactions
Component of the immune system
Involved in fluid, electrolyte, and acid/base balance
Many hormones are either proteins or transported through protein carriers
Provide structure (bones, teeth, etc.) and required for maintenance of tissues and organs.
Can be oxidized for energy
How are we able to measure protein metabolism?
This is done by infusing a known amount of amino acids with stable isotope tracers into the subject. The stable isotopes allow researchers to track protein synthesis, degradation, and oxidation.
Which amino acids can be used as stable isotope tracers? Why?
Only essential amino acids (EAA) can be used as stable isotope tracers because non-essential amino acids can be synthesized. This would result in an unknown portion of amino acids coming from the stable isotope and some coming from synthesis. By using only essential amino acids, the researcher knows the level of amino acid pools (only what was given to the subject).
Since branched chain amino acids are preferentially oxidized, the amino acid with the stable isotope tracer should be an essential amino acid and a branched chain amino acid.
What does the rate of appearance and disappearance correlate to?
In the blood, the rate of disappearance of the tracer indicates protein synthesis while the rate of appearance indicates degradation.
What aspect of protein metabolism can we measure through CO2 production?
Oxidation of proteins can also be measured through CO2 production.




