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PMG maldonado UTTEEP > exam 3 > Flashcards

exam 3 Flashcards

1
Q

The transposons are also name as a) jumping genes b) mobile genetic elements c) junk DNA d) a and b e) a, b and c

A

e) a, b and c

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2
Q

Transposons can contribute to genetic diversity by causing mutations

A

true

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3
Q

Retrotransposons copy themselves to RNA (transcription), but instead of being translated the RNA is copied into DNA by a reverse transcriptase

A

a) True

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4
Q

The transposase cut and paste the transposon

A

a) True

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5
Q

Insertion sequence elements only encode gene products required for transposase synthesis and regulation

A

a) True

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6
Q

Transposon with inactive transposase continue to jump through the genome

A

a) True

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7
Q

Composite transposon only is composed by two IS sequences

A

a) True

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8
Q

The target DNA has a short direct repeat that bracket the transposon

A

a) True

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9
Q

During the transposition of the insertion elements the repeat in the target DNA is not duplicated

A

b) False

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10
Q

In the Out-site end Transposition, each IS element can transpose independently as long as the transposase acts on both of its ends

A

a) True

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11
Q

13) The Inside end transposition occur in
a) Composite transposon
b) Non-composite transposon
c) IS elements

A

a) Composite transposon

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12
Q

14) Consequences of the inside end transposition

  • a) Creation of a new composite transposon
  • b) Deletion and inversion to a nearby target on the same DNA
  • c) a and b
  • d) None of the options
A

c) a and b

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13
Q

15) A common way of delivering transposons is to use ______________ that cannot replicate in the recipient cells. pGP704 is an AmpR plasmid that is unable to replicate unless the Pi protein (encoded by the pir gene) is provided in the recipient cell. a) Plasmid b) Phage c) Suicide Vector

A

c) Suicide Vector

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14
Q

The distinction between an insertion sequence and a composite transposon is that the insertion sequence only contains genes for the mobility of the sequence

A

a) True

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15
Q

Replicative mechanism, part of the transposon replicates during transposition resulting in two copies of the transposon

A

b) False

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16
Q

Cut-and-Paste or conservative or non-replicative mechanism, the transposon is removed from one place and inserted into another

A

a) True

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17
Q

Cointegrates are obligate intermediaries in copy and paste transposition

A

b) False

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18
Q

Resolvase promotes recombination

A

a) True

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19
Q

In the cut and paste mechanism the transposase makes a double-stranded beak at the ends of the transposon

A

a) True

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20
Q

Transposition is regulated by Dam methylation

A

a) True

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21
Q

24) __________________ copy themselves to RNA (transcription), but instead of being translated the RNA is copied into DNA by a reverse transcriptase (often coded by the transposon itself) and inserted back into the genome
a) Class I transposon
b) Class II transposon
c) Retrotransposons
d) a and c
e) b and c

A

Retrotansposons

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22
Q

The definitive experiment proving this mechanism for transposon Tn10 was done by Bender and Kleckner

A

a) True

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23
Q

In both transposition mechanism the cut 5’ ends of the target DNA are join to the free 3’ end of the transposon and the target DNA 3’ end is used as a primer for replication until the free 5’ end is reached

A

a) True

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24
Q

One amino acid change in the transposase can convert a non-replicative transposon into a replicative transposon

A

a) True

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25
Q

Typically, the transposition frequency is decrease when a transposon enters a naive host or immediately after DNA replication

A

b) False

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26
Q

Hemimethylation is a signal that DNA has just replicated

A

a) True

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27
Q

In hemimetilated DNA the binding of RNA pol to the dam gene promoter decrease and the transposition increase

A

b) False

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28
Q

In both recombination models, Holliday Double-strand Invasion and Double Break Repair the formation of two Holidays junction is carried out

A

b) False

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29
Q

In the Isomerization hydrogen bonds are broken and therefore energy is used.

A

b) False

30
Q

36) It is movement of the Holliday junction up and down the DNA by breaking and reforming the hydrogen bonds between the bases a) Resolution b) Isomerization c) Branch migration

A

c) Branch migration

31
Q

In the single strand invasion model of genetic recombination one strand of the DNA molecules is cut and invade the other double strand DNA molecule until it finds its complementary sequence

A

a) True

32
Q

38) RecBCD is required for
a) Recombination
b) Degradation of foreign linear DNA
c) Restart replication
d) a, b and c

A

d) a, b and c

33
Q

Different species of bacteria have different sequences for χ site a) True b) False

A

True

34
Q

34) Pathway used to prepare single stranded DNA for D-loop formation in E. coli a) RecBCD pathway b) RecF pathway c) RecE pathway

A

b) RecF pathway

35
Q

17) Mention the properties of the transposable elements
a) Movement is not totally random, not capable of self-replication, Transposition mediated by site-specific recombination and Transposition can be accompanied by duplication
b) Movement is not totally random, not capable of self-replication, Transposition mediated by site-specific recombination
c) Movement is not totally random, it is capable of self-replication, Transposition mediated by site-specific recombination and Transposition can be accompanied by duplication

A

a) Movement is not totally random, not capable of self-replication, Transposition mediated by site-specific recombination and Transposition can be accompanied by duplication

36
Q

RecA is required both RecBCD and RecF pathway a) True b) False

A

True

37
Q

36) The diagram bellow represents which type of recombination:
a) Holliday double strand invasion model
b) Single strand invasion model of genetic recombination
c) Double-Strand Break Repair Model

A

b) Single strand invasion model of genetic recombination

38
Q

37) ____________ is the recombination of DNA sequences having nearly the same nucleotide sequence and involving Rec A proteins.
a) homologous recombination
b) heterologous recombination
c) transfer of genetic information
d) Transposition

A

a) homologous recombination

39
Q

38) It is movement of the Holliday junction up and down the DNA by breaking and reforming the hydrogen bonds between the bases
a) Synapse
b) Branch migration
c) Isomerisation

A

b) Branch migration

40
Q

In the holliday double strand invasion model if the same strands are cleaved a second time then non-recombinant DNA molecule are generated, but they each contain a region of heteroduplex DNA that spans the region of branch migration

a) True
b) False

A

a) True

41
Q

40) The rearrangement between the strands after the formation of the Holliday junction called
a) Synapse
b) Branch migration
c) Isomerisation
d) Resolution

A

c) Isomerisation

42
Q

________________ is the cut and ligation of DNA strand in the Holliday junction

a) Synapse
b) Branch migration
c) Isomerisation
d) Resolution

A

d) Resolution

43
Q

42) ____________________ recombinant molecules are generated when each molecule contains a region of heteroduplex DNA that spans the region of branch migration (configuration II)
a) holliday double strand invasion model
b) holliday single strand invasion model
c) Double break repair model

A

a) holliday double strand invasion model

44
Q

43) Recombination depends of the holliday junction configuration when they are resolved, If both junctions with the same configuration-either one or II, then it is not crossover and no recombination happen
a) holliday double strand invasion model
b) holliday single strand invasion model
c) Double break repair model

A

c) Double break repair model

45
Q

45) ______________ is required for recombination (prepare the single strand end of DNA to invade the other double-strand DNA – first step in recombination), degradation of foreign linear DNA and in restart the replication when the replication fork stalls at single or double-strand-breaks in the template DNA.
a) RecA
b) SSB
c) RecBCD
d) RecQ

A

c) RecBCD

46
Q

The χ sequence is only recognizing in one strand of DNA a) True b) False

A

True

47
Q

χ site inhibit RecD subunit and stimulate 5’to 3’ activity a) Tre b) False

A

True

48
Q

48) ______________ is formed when two DNA molecules are joined through the formation of the D-loop
a) Synapse
b) Branch migration
c) Isomerisation
d) Resolution

A

Synapse

49
Q

49) _______________ bind to ssDNA and form nucleoprotein filaments
a) RecA
b) SSB
c) RecBCD
d) RecF

A

RecA

50
Q

50) _____________ force the ssDNA into the helical conformation
a) RecA
b) SSB
c) RecBCD
d) RecQ
e) a and b

A

a) RecA

51
Q

51) It has ATP and DNA binding activities and weak ATPase activity
a) RecA
b) SSB
c) RecBCD
d) RecF
e) a and b

A

RecF

52
Q

__________________ pass over the site only on the other strand making the orientation of the χ sequence important

a) RecA b) SSB c) RecBCD d) RecF

A

RecBCD

53
Q

54) The following diagram represents

  1. Holliday double strand invasion model
  2. Holliday single strand invasion model
  3. Double break repair model
A

c) Double break repair model

54
Q

55) chi site stop RecD 3’ to 5’ nuclease activity and stimulate 5’to 3’ nuclease activity, leaving a single stranded 3’ end
a) True b) False

A

a) True

55
Q

56) ______________ is required for recombination (prepare the single strand end of DNA to invade the other double-strand DNA – first step in recombination), degradation of foreign linear DNA and in restart the replication when the replication fork stalls at single or double-strand-breaks in the template DNA.
a) RecA
b) SSB
c) RecBCD
d) RecQ
e) RecE

A

c) RecBCD

56
Q

57) In the Holliday double strand invasion model if the same strands are cleaved a second time then non-recombinant DNA molecule are generated, but they each contain a region of heteroduplex DNA that spans the region of branch migration
a) True b) False

A

a) True

57
Q

It is movement of the Holliday junction up and down the DNA by breaking and reforming the hydrogen bonds between the bases

a) Sinapse
b) Branch migration
c) Isomerisation

A

b) Branch migration

58
Q

______________ is formed when two DNA molecules are joined through the formation of the D-loop a) Synapse b) Branch migration c) Isomerisation d) Resolution

A

a) Synapse

59
Q

DnaK binds to sigma-32 and makes sigma-32 more susceptible to protease degradation. a) True b) False

A

a) True

60
Q

Sigma-32 is transcriptional regulated a) True b) False

A

False

61
Q

Sigma-32 binds to the RBS of its own mRNA and blocks access of the ribosome a) True b) False

A

True

62
Q

CAP is responsible for the global regulation of carbon utilization. a) True b) False

A

true

63
Q

The figure below represents

a) Class I CAP-activated promoters
b) Class II CAP-activated promoters
c) Class III CAP-activated promoters

A

b) Class II CAP-activated promoters

64
Q

In heat shock, the Hsp bind to denatured proteins and either help to refold them into their active conformations or, if the protein is extensively denatured, chaperones target these proteins for destruction. a) True b) False

A

true

65
Q

67) Recombination Pathway used to prepare single stranded DNA for D-loop formation in E. coli and initiate recombination at single-stranded gaps in DNA.
a) RecBCD pathway
b) RecF pathway
c) RecE pathway

A

b) RecF pathway

66
Q

69) umu C, umu D gene family and Rec A proteins are involve in a) BER b) NER c) SOS repair d) Recombination repair

A

c) SOS repair

67
Q

70) Dimer repair mechanism include
a) Excision repair
b) Photoreactivation
c) Recombinant repair
d) all of these

A

d) all of there

68
Q

71) Recombinational repair is often due to
a) Incorporation of many incorrect nucleotide by DNA polymerase
b) Many cytidine dimer and associated large gaps in a strand
c) many thymidine dimer formation and associated large gaps in a strand
d) all of these

A

c) many thymidine dimer formation and associated large

69
Q

73) In base excision repair, the lesion is removed by __________
a) DNA glycosylase
b) Excisionase
c) Transposase
d) DNA polymerase

A

a) DNA glycosylase

70
Q

74) In nucleotide excision repair, an endonuclease makes nicks on either side of the lesion, which is then removed to leave a gap. This gap is filled by __________ to makes the final phosphodiester bond. I.DNA polymerase II.DNA ligase III. DNA glycolase
a) I only
b) II only
c) III only
d) I and II
e) I, II, and III

A

d) I and II

71
Q

75) The enzyme of Escherichia coli is a nuclease that initiates the repair of double-stranded DNA breaks by homologous recombination.
a) RNA polymerase
b) DNA polymerase
c) DNA ligase
d) RecBCD
e) DNA glycolase

A

d) RecBCD

PMG maldonado UTTEEP (6 decks)

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