Exam 3 Flashcards
In female birds, elevation of serum _____ above a threshold level causes a GnRH surge, leading to an ___ surge, and ovulation.
- Progesterone
- LH
What is the source of progesterone that can cause ovulation in birds when it is above a threshold level?
-F1 follicle
What two things does FSH doe in mammals and birds?
- Stimulate granulosal cells
2. Promote follicular development
What does LH do in mammals and birds?
- Stimulates thecal cells
- When granulosa cells of a preovulatory follicle acquire LH receptors, ovulation will occur in response to the LH surge
In female mammals, granulosa cells convert ____ to _____ and are the primary source of follicular _____. Thecal cells are the primary source of ______.
- Androgen
- Estrogen
- Estrogen
- Androgen
True or False: the chicken follicle has no antrum and no follicular fluid.
True
What is the chicken follicle filled with?
Yolky oocyte
What is ovulation in the bird?
The release of the ovum with yolk from the follicle
The interval from the LH surge to ovulation is ___ - ____ hours. The interval from ovulation to oviposition is ______ hours. The interval from oviposition to the next ovulation is _____ hours. Under what condition is this not true?
- 4-8
- 24
- .5
- If it is the last egg in the clutch
What occurs to the post-ovulatory follicle of the bird?
-It will become inactive and be reabsorbed
Atresia occurs in ___% of primordial and growing follicles of the laying hen.
90
The _____ receives the ovulated oocyte and the yolk. What other two things occur here?
- Infundibulum
1. Fertilization occurs here from sperm stored in storage tubules
2. Chalazae formed here
Describe the chaliziferous layer.
-Fibrous layer of the albumen that covers the entire yolk. It is twisted at both ends of the yolk to form thick rope-like structures that allow the yolk to rotate and keep it in the center of the egg. It is formed in the infundibulum.
The egg spends ________ in the infundibulum, ______ in the magnum, ________ in the isthmus, ______ in the shell gland, and ______ in the vagina.
- 15 minutes
- 3 hours
- 1 hour 15 minutes
- 18-25 hours
- 1 minute
In the ______ the albumen is secreted. What does it do? What are the two layers?
-Magnum
-Surrounds and protects the yolk and blastoderm that gives rise to the embryo
-Inner layer: Thick albumen
Outer Layer: Thin Albumen
The _____ is where the soft shell membrane is added around the albumen.
-Isthmus
What occurs in the shell gland?
-The hard shell is added
Where is the primary site of sperm storage tubules? What is their purpose?
- Utero-vaginal junction
- To protect sperm from the hen’s immune system, preserve energy, and prolong the sperm’s life span
What occurs in the vagina of the hen?
-The mucin coating is added to the egg
The _____ inverts for oviposition.
Cloaca
Why does the yolk of the egg have light and dark layers?
- When the hen eats, she adds more fat, creating dark yellow layers
- When the hen is not eating, she adds more protein, adding light white layers
A ____ is consecutive days over which eggs are laid.
-Clutch
What three things does optimal spermatogenesis require?
- Adequate secretion of GnRH from the hypothalamus
- FSH and LH secretion from the anterior pituitary
- Secretion of gonadal steroids such as testosterone
What are tonic GnRH secretion patterns like? What are LH and FSH secretion patterns like?
- GnRH: Frequent, intermitten pulses lasting several minutes
- LH and FSH: Follow GnRH pulses with 4-8 pulses per day. FSH is lower in amplitude and longer in duration than LH.
Why are patterns of FSH and LH secretion different?
- Inhibit inhibits only FSH, so FSH amplitude is lower
2. FSH has a longer half-life. so duration is longer
Describe the testosterone secretion patterns and why the patterns are pulsatile.
- Follow LH pulses
- If LH levels were continuously high, superstimulation would occur and lead to desensitizing of the LH receptors. This way, LH stimulates a burst of testosterone and then the testes can relax and respond to the next burst.
_____ concentrations of androgens withing the _____ _______ are essential for optimal spermatogenesis
- High
- Seminiferous Tubules
Androgens such as testosterone and dihydrogentestosterone are produced by ____ cells. What is their job?
- Leydig
- To maintain spermatogenesis by decreasing germ cell degeneration to increase the number of sperm cells produced
___ from the anterior pituitary stimulates leydig cells to produce _____.
- LH
- Androgen
____ from the anterior pituitary stimulates sertoli cells to increase the production of _____ ______ _____.
- FSH
- Androgen binding protein.
LH stimulates ____ cells, while FSH stimulates _____ cells.
- Leydig
- Sertoli
Sertoli cells make androgen binding protein. What two other things doe they produce?
- Inhibin for negative feedback
2. Estrogen
What does androgen binding protein do and why?
Binds androgen to concentrate it within the seminiferous tubules for optimal spermatogenesis
What does androgen inhibit?
The hypothalamus and anterior pituitary for decreased GnRH, FSH, and LH. Decreased LH leads to decreased androgen production.
What does estrogen inhibit?
Gonadotropin secretion at the hypothalamus and anterior pituitary
Inhibin is produced by ____ cells and inhibits _____ at the ____ ____.
- Sertoli
- FSH
- Anterior Pituitary
____ cells, also called nurse cells, reach from the basement membrane of seminiferous tubules to the lumen. What doe they do? What three things do they secrete?
- Sertoli
- Help sperm develop and mature
- Fluid for sperm transport
2. Androgen binding protein
3. Inhibin
- Fluid for sperm transport
What is the spermatogenic cell lineage?
Spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, spermatozoa
____ are primitive germ cells resting on the basement membrane. They are ___n and replicate by ___.
- Spermatogonia
- 2
- Mitosis
____ ______are larger, rounded cells derived form spermatogonia that move away from the basement membrane. They are ___n and will undergo _____.
- Primary spermatocytes
- 2
- meiosis
______ _____ result from the first meiotic division of primary spermatocytes. They are ___n.
- Secondary spermatocytes
- 1
_____ result from the second meiotic division. They are _____n and are the smallest cells that are close to the lumen.
- Spermatids
- 1
_____ result from the morphological development of spermatids, in which the head and tail are visible.
-Spermatozoa
In males, you get ____ spermatids from ____ primary spermatocyte. In females, you get ___ polar bodies and ____ ovum from one primary ooctye.
- 4
- 1
- 2
- 1
Mitotic divisions result in _____ of spermatogonia. As they divide, ____ changes occur.
- proliferation
- Maturational
During meiosis, a primary spermatocyte becomes ___ spermatids.
-4
What occurs during differentiation of sperm?
- Spermatids mature into spermatozoa
- Changes include condensation of nuclear chromatin, formation of flagellum, and development of the acrosome.
The _____ is a balloon of enzymes over top of the sperm head that is acquired during differentiation.
Acrosome
True or False: Blood and lymph penetrate the seminiferous tubules
False, because of the blood-testis barrier
What are the components of the blood-testis barrier?
- Peritubular cells surrounding seminiferous tubules
- Sertoli cell tight junctions are the principal barrier
What are the three functions of the blood-testis barrier?
- Exclude substances that may be harmful to spermatogenesis such as toxins
- Retain high levels of substances such as androgen binding protein
- Immunological isolation of developing spermatids, since haploid cells are recognized as foreign
Primary spermatocytes udergo _____ ____ and are _____. Secondary spermatocytes undergo ____ ____ and are _____.
- Meiosis I
- Diploid
- Meiosis II
- Haploid
______ _____ is one of the strongest drives in animals that often takes precedence over other drives such as eating and sleeping.
-Reproductive behavior
When do males and females with estrous cycles show reproductive behaviors?
- Males: Anytime after puberty
- Females: In estrus
What are the three distinct stages of reproductive behavior in the male and describe each.
- Precopulatory stage: Male is searching for a sexual partner, courtship, arousal, erection, penile protrusion out of the sheath
- Copulatory stage: Mounting, intromission of penis into vagina, ejaculation
- Postcopulatory stage: Dismount, refractory period, memory
The _____ period is the period of time when a second copulation will not take place.
Refractory
In most species, female reproductive behaviors are promoted by high levels of circulating ____.
Estrogen
What are the precopulatory and copulatory behaviors of the female and describe them.
- Precopulatory: 1. Attractivity: signals that attract the male such as posture, vocalizations, and pheromones 2. Proceptivity: Behaviors stimulate the male to copulate such as maintaining proximity, presentation of genetalia, increase in activity, and mounting other females
- Copulatory: Receptivity behaviors may include accepting the male, standing to be mounted, lordosis, and deviation of the tail
What courtship behaviors are common in several species?
- sniffing of the vulva by the male
- urination by the female in the presence of the male
- flehmen behavior of the male
- Male’s chin resting on the hind end of the female
- Increased phonation
Name three sustained copulators.
- Boar
- Dog
- Camelid