Exam 3 Flashcards

1
Q

What are the three eras of microbiology? The Big 3 so to speak?

A

the 1600s and before, pre-microbe era
the 1880s and before, pre-germ theory era
from the 1880s to now, post germ theory/ the modern era

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2
Q

How did vaccines lead to the total eradication of smallpox?

A

Vaccines help the body develop immunity to the live virus and this method was used by the world and by 1980, the disease smallpox was considered eradicated by the WHO.

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3
Q

How was variolation practiced by the Chinese?

A

16th century
Dried mixture of scabs
Blown up nose using a long tube

In the 16th century, a mixture of dried scabs from an individual that had a mild version of smallpox was blown up the nose and this is how they practiced inoculation.

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4
Q

How was variolation practiced by the Turks?

A

17th century
Fluid from fresh pustule
Rubbed into small incision in the skin

In the 17th century, the Turks took the fluid from a pustule of an infected person and rubbed that into a small incision on a patch of skin to inoculate an individual.

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5
Q

What was Jenner’s approach to smallpox vaccination?

A

He used the pus from an individual infected with cowpox to inoculate another individual from smallpox.

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6
Q

What is the medical based opposition to vaccination?

A

When people don’t trust physicians and suspect that they could be injected with poison instead of a true vaccine.

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7
Q

What is the major/ minor religious based opposition to vaccination?

A

Major- People decline medical help for major diseases and instead choose to believe that the disease will be cured or prevented through prayer. This is also an ego trip- they believe declining medicinal aid affirms the idea of human perfection. ((Think of the Monk religious cult episode for major opposition))

Minor- Vaccines could have fetal-derived products or vaccines contain residual pork products.

FDP—Roman Catholicism
RPP—Orthodox Jews

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8
Q

What is the safety based opposition to vaccination?

A

People were concerned about the unsafe additives or stabilizers contained in vaccines/// Allergic reactions are also a cause of concern.

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9
Q

Who are nutters?

A

People that spread misinformation and create dissension to vaccination, usually via baseless claims that only spark outrage.

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10
Q

What is the antivax movement?

A

Where vaccines were linked to giving children autism.

Parents with autistic children were no longer participating in regular vaccination of their children and parents without autistic children chose to not vaccinate their children in the first place to avoid that “danger”.

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11
Q

When did HIV start infecting humans?

Where did HIV start infecting humans?

A

1920s

DRC (Democratic Republic of Congo)

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12
Q

When was AIDS first recognized as a disease?

When did the silent spread of AIDS end?

A

The silent spread of HIV/AIDS ended in 1981.

AKA

HIV/AIDS was recognized as a disease in 1981.

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13
Q

Why was ‘96 described as a miracle year in terms of HIV/AIDS?

A

A cure was found to stop the transmission of HIV from an HIV positive individual.

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14
Q

Once an individual is HIV positive, can they be cured of the virus?

Can we prevent them from developing AIDS?

A

No, they cannot be cured of the virus, but the symptoms can be suppressed.

They can be prevented from developing AIDS (the endemic version of this virus)

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15
Q

Why is it not complicated to maintain a healthy gut microbiome?

A

Our microbiome is made of microbes that display conserved molecular patterns. These patterns are complicated, but giving these patterns proper fuel is easy- eating probiotic and prebiotic foods provides the microbes the necessary energy to sustain a healthy gut microbiome.

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16
Q

What are prebiotics and how do they help maintain a healthy microbiome?

A

Prebiotics are food for GOOD bacteria and they help maintain a healthy microbiome by controlling the environment of the intestinal microbiome. The prebiotics facilitate the production of SCFAs and some examples of them are GREEN- garlic, leafy greens, and dandelion greens.

17
Q

What types of diseases have fecal transplants been shown to be a viable treatment option?

A

IBS and CC

Irritable Bowel Syndrome and Chronic Constipation

18
Q

What are the key ideas of virus transmission from animals to humans.

A

Viruses that can be transmitted to humans from animals are called zoonotic diseases.

An estimated 3 out of 4 emerging infectious human diseases are caused from animal to human transmission.

19
Q

How do SARS-COV2 and HIV viruses enter human cells?

A

The SARS viral protein interacts with ACE2 receptor to enter human cells.

HIV enters the cell via CD4.

20
Q

How do viruses use host cells to replicate?

A

Viruses use the host cell machinery to replicate and produce new viral particles to further invade the host.

21
Q

What are the different types of vaccines?

A

Inactivated, Live/ Weakened, Nucleic Acid vaccines

22
Q

What is an inactivated vaccine?

A

An inactivated vaccine uses the killed version of the germ that caused the disease

23
Q

What is a live vaccine?

A

A weakened/ live vaccine contain a small amount of the weakened live virus and need to be used with caution.

24
Q

What is a nucleic acid vaccine?

A

They are DNA or mRNA vaccines that produce strong, long term immunity

25
Q

Why is the immune response to HIV and designing vaccines against HIV pose problems?

A

There is an extraordinary demand on the antiHIV neutralizing ABS so only a few of them can reach the CD4 binding site.

26
Q

Which immune cells clear the virus infection?

A

Adaptive immune cells- B + T cells & CD4/ CD8
Innate immune cells- dendritic cells, natural killer cells, macrophages ((DC, NK, MAC))

27
Q

What does the clonal nature of adaptive immunity, aka the B cell clonal selection theory, relate to the very high number of antibody secretion by B cells?

A

B cells express a receptor on their surface, are activated by an antigen, and this triggers the mass multiplication of antigens.

So, B cells express a unique receptor on their surface, binds to a specific antigen, the receptor then activates the B cell and triggers multiplication. “The chosen one finds its partner”

Once activated, B cells trigger the multiplication of massive amounts of antibodies that swarm their target.

28
Q

What is the role of T cell help in B cell antibody production?

A

B cells need T helper (CD4) cells for antibody production.

B CELLS NEED T HELPER CELLS FOR ANTIBODY PRODUCTION. ANTIBODIES SPECIFIC TO THE VIRUS.

29
Q

What are the differences in primary and memory immune response?

A

Memory immune response is faster and more robust than primary immune response.

Primary immune response= innate immunity; it’s what the immune system uses as it’s first line of defense without too much specificity. Think throwing all of its tools at a problem without knowing the specifics of what’s going on. This is where the DC, NK, and MP trio come in. (Dendritic cell, natural killer, macrophage)

Memory immune response= adaptive immunity; it’s the much more robust response and here’s where B & T cells come into the mix. Here, the virus can be neutralized and killed more effectively.

30
Q

As a key idea, molecular recognition is the basis of both microbe-immune coexistence and immune attack against microbes. What are some examples of this relationship in your immune system?

A

There is a virus-receptor relationship,
Molecular patterns relationship, and
Protein-protein interactions inside the cells.

31
Q

As a key idea, the power of adaptive immunity is in a large repertoire of cells that can each target specific microbes.

A

As an example, an antigen-specific B cell clone multiplies into billions to make massive amounts of the antibody against that antigen.

32
Q

So how do these B cell clones multiply?

A

The antibodies attach to the B cell membrane before they are secreted and then triggers the cell to begin multiplication; that’s the basis for biological processes inside the cells.

33
Q

Key idea: Vaccines make your body expereince the infection without getting infected.

A

Parts and pieces of the virus (antigens) show the immune system how the enemy looks like (molecular recognition)

A vaccine can only happen if it mimics a pathogen, the immune system will see the structures shown by the vaccine and become stimulated to begin creating a protein.

34
Q

Key Idea: Errors in genes can cause immune deficiency or autoimmunity; HOW?

A

There are mutations that can disable an important immune gene= immunodeficiency

Genetic variations that overactivate the immune system= autoimmune diseases, SNPs, gain of function