Exam 3

Question 1

Name the levels of autolysosome formation

Answer 1

1. Omegasome
2. Phagophore
3. Autophagosome
4. Autolysosome

Question 2

Describe autophagosome structure

Answer 2

• Double layer membrane
• Mammalian autophagosomes are 500-1500 nm
• Their formation is controlled by Atg genes and LC3 complexes
• Autophagosomes fuse with lysosomes to form autolysosomes
• Material in the autophagosome is degraded by lysosomal hydrolases