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Microbial Physiology > Exam 3 > Flashcards

Exam 3 Flashcards

1
Q

The fructose bisphosphate aldolase pathway (or EMP) is the only method of glycolysis.

A

False

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2
Q 
"Having the appropriate enzymes, which of the following can be used by bacteria for energy production?"
Amylopectin
Cyclodextrin
Fatty acids
Triglycerides
all of the above can be used
A

all of the above can be used

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3
Q

Amylotransferase releases a molecule of glucose from maltose, what is the next step in the metabolism of maltose through the EMP pathway?”

A

It is phosphorylated

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4
Q

“During growth when glucose is plentiful, thoughtful bacteria will plan ahead to do what?

A

Store glucose as glycogen

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5
Q

What is the ultimate goal of glycolysis?

A

Convert sugars to pyruvate.

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6
Q 
Having the appropriate enzymes, which of the following can be used by bacteria for energy production?"
Maltose
Mannitol
Sorbital
Mannose
all of the above can be used
A

all of the above can be used

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7
Q

What is the major metabolic pathway?

A

Embden-Meyerhof-Parnas Pathway

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8
Q

The process of converting glucose to pyruvate is called …

A

Glycolysis

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9
Q

When grown on carbon poor sources such as acetate or pyruvate, bacteria will utilize what special pathway to regenerate OAA?”

A

Glyoxylate shunt

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10
Q

When mannose is taken up by bacteria it is phosphorylated by hexokinase, what is the next step in the metabolism of mannose through the EMP pathway?

A

It is isomerized to glucose-6-P

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11
Q

Which pathway generates the unique sugar KDPG?

A

Entner-Doudoroff pathway

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12
Q

What is the purpose of glycolysis?

A

make pyruvate

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13
Q

What unique sugar is generated by the Pentose Phosphate pathway, is a common component of the inner core region of LPS?

A

Sedoheptulose

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14
Q

Alternate pathways of glycolysis (other than EMP) convert glucose to what intermediate in E. coli?

A

6-Phosphogluconate Gluconate

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15
Q

Which enzyme catalyzes the split of fructose-1,6-P2 into glyceraldehyde-3-P and DHAP in the EMP pathway?

A

Fructose bisphosphate aldolase

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16
Q

We discussed several pathways of glucose metabolism, which is/are the most energetically favorable for the bacteria?

A

Embden-Meyerof-Parnas pathway

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17
Q

Which pathway generates the unqiue sedoheptulose sugar which is a percursor for the heptose used in LPS?

A

Hexose monophosphate (pentose phosphate) pathway

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18
Q

Which pathway generates ribose used as the base in DNA and RNA?

A

Pentose phosphate pathway

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19
Q

What is the goal of the TCA/Kreb’s cycle?

A

Makes NADH + H+

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20
Q

Pyruvate enters the TCA/Kreb’s cycle by bindinig to Oxaloacetate making a 6-carbon citrate. Through the cycle it losses 3 carbons to regenerate oxaloacetate, where do they go?

A

Removed as CO2

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21
Q

Under anaerobic conditions, what happens to the TCA/Creb’s cycle?

A

It operates as a branched pathway

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22
Q

In EMP pathway of glycolysis is involved with net gain of

A

2 ATP+ 2NADH

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23
Q

If a baceria is growing on acetate only, what happens to the TCA/Kreb’s cycle?

A

The glyoxylate shunt is utilized

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24
Q

Pentose Phosphate pathway involves

A

generation of 5-C sugars as Ribose

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25
Q

Intermediates in TCA cycles are involved in

generation of amino acids as arginine, proline
generation of ATP
Generation of NADH
All the statements are true

A

All the statements are true

26
Q

Why must a bacteria ferment if it can’t utilize oxygen?

A

To regenerate NAD+

27
Q

Which of the following is NOT true about Electron transport chain

A

It consist of just one protein ATP Synthase

28
Q

Oxidative phosphorylation is same as substrate level phosphorylation ?

A

false

29
Q

Transport of electrons down the electron transport chain generates.

A

proton motive force

30
Q

What molecules generated during glycolysis and the Kreb’s cycle deliver electrons to the electron transport chain?

A

NADH + H+

31
Q

Utilization of the PMF to create energy is called what?

A

Oxidative phosphorylation

32
Q

In electron transport chain CO2 can be used as terminal electron acceptor?

A

False

33
Q

Which of the following components is a membrane-embedded motor with channels connecting the inside and outside of a bacterial cell?

A

F0-F1 ATP synthase

34
Q

During glycolysis, ATP is made by what process?

A

substrate-level phosphorylation

35
Q

A high level of NADH would have which effect on respiration

A

Decrease TCA cycle, induce fermentation

36
Q

Catabolic processes make molecules while anabolic processes break molecules down.

A

False

37
Q

In the presence of oxygen, anaerobic organisms utilize which:

A

Glyoxylate shunt

38
Q

In the presence of oxygen, anaerobic organisms utilize which:

A

Glyoxylate shunt

39
Q

In the presence of oxygen, aerobic organisms utilize the TCA cycle for what purpose?

A

make NADH+H

40
Q

We discussed several pathways of glucose metabolism, which is a pathway of fermentation?

A

Phosphoketolase pathway

41
Q

Which is not a possible end product of fermentation?

Lactate
Ethanol
Butanol
Formic Acid
All are end products
A

All are end products of fermentation

42
Q

Which of the following cannot use oxygen for respiration?

A

Anaerobe

43
Q

We discussed several pathways of glucose metabolism, which involves the transaldolase and transketolase catalyzed movement of carbons between 5-carbon sugars?

Pentose Phosphate pathway
Entner-Doudoroff pathway
Phosphoketolase pathway
EMP pathway

A

Pentose Phosphate pathway

44
Q

In the presence of oxygen, aerobic organisms utilize the TCA cycle for what purpose?

A

make NADH+ H+

45
Q

We discussed several pathways of glucose metabolism, which generates the least amount of ATP (not involving TCA or ETC)

A

Entner-Doudonoff pathway

46
Q

Which enzyme phosphorylates glucose in the EMP pathway?

A

Hexokinase

47
Q

Substrate level phosphorylation involves synthesis of ATP and inorganic phosphate (Pi)

A

False

48
Q

Which of the following does not happen in the Embden-Meyerhof-parnas pathway of glycolysis:

A

generation of PMF

49
Q

The F0-F1 ATPase can utilize what differential to generate ATP under alkaline environmental conditions?

A

Glucose

50
Q

The F0-F1 ATPase can utilize what differential to generate ATP under alkaline environmental conditions?

A

Glucose

51
Q

Which enzyme catalyzes the removal of phosphate from PEP yielding the ultimate end product of glycolysis in EMP pathway?

A

Pyruvate kinase

52
Q

What unique sugar is generated by the EMP pathway?

A

KDPG

53
Q

What is the goal of fermentation?

A

make NAD+

54
Q

why is fructose-6-p phosphorylated again using more energy:

A

when split into glyceraldehyde and dihydroxyacetone, Both products will be phosphorylated

55
Q

What is the goal of glycolysis?

A

make pyruvate

56
Q

Which of the following is false regarding the electron transport chain (ETC) in bacteria :

A

the ETC proteins pump protons out of the cell

57
Q

.Lactose is a disaccharide of glucose and galactose, upon cleavage by beta-galactosidase both sugars are phosphorylated. The glucose-6-p is processed through the EMP pathway, what is the next step for the galactose-1-p:

A

It is epimerized to glucose (while bound to the carrier UDP)

58
Q

.Lactose is a disaccharide of glucose and galactose, upon cleavage by beta-galactosidase both sugars are phosphorylated. The glucose-6-p is processed through the EMP pathway, what is the next step for the galactose-1-p:

A

It is epimerized to glucose (while bound to the carrier UDP)

59
Q

The F0-F1 ATPase can utilize what differential to generate ATP under neutral pH environmental conditions?

A

Proton

60
Q

Which of the following is FALSE about the F0-F1 ATPase?

A

F0-F1 ATPase requires active energy input to generate ATP

61
Q

Which of the following can use oxygen for respiration?

A

Aerobe and Facultative anaerobe

62
Q

Which enzyme rephosphorylates fructose 6-phosphate in the EMP pathway?

A

Phosphofructokinase

Microbial Physiology (2 decks)

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