Exam 3 Flashcards
The fructose bisphosphate aldolase pathway (or EMP) is the only method of glycolysis.
False
"Having the appropriate enzymes, which of the following can be used by bacteria for energy production?" Amylopectin Cyclodextrin Fatty acids Triglycerides all of the above can be used
all of the above can be used
Amylotransferase releases a molecule of glucose from maltose, what is the next step in the metabolism of maltose through the EMP pathway?”
It is phosphorylated
“During growth when glucose is plentiful, thoughtful bacteria will plan ahead to do what?
Store glucose as glycogen
What is the ultimate goal of glycolysis?
Convert sugars to pyruvate.
Having the appropriate enzymes, which of the following can be used by bacteria for energy production?" Maltose Mannitol Sorbital Mannose all of the above can be used
all of the above can be used
What is the major metabolic pathway?
Embden-Meyerhof-Parnas Pathway
The process of converting glucose to pyruvate is called …
Glycolysis
When grown on carbon poor sources such as acetate or pyruvate, bacteria will utilize what special pathway to regenerate OAA?”
Glyoxylate shunt
When mannose is taken up by bacteria it is phosphorylated by hexokinase, what is the next step in the metabolism of mannose through the EMP pathway?
It is isomerized to glucose-6-P
Which pathway generates the unique sugar KDPG?
Entner-Doudoroff pathway
What is the purpose of glycolysis?
make pyruvate
What unique sugar is generated by the Pentose Phosphate pathway, is a common component of the inner core region of LPS?
Sedoheptulose
Alternate pathways of glycolysis (other than EMP) convert glucose to what intermediate in E. coli?
6-Phosphogluconate Gluconate
Which enzyme catalyzes the split of fructose-1,6-P2 into glyceraldehyde-3-P and DHAP in the EMP pathway?
Fructose bisphosphate aldolase
We discussed several pathways of glucose metabolism, which is/are the most energetically favorable for the bacteria?
Embden-Meyerof-Parnas pathway
Which pathway generates the unqiue sedoheptulose sugar which is a percursor for the heptose used in LPS?
Hexose monophosphate (pentose phosphate) pathway
Which pathway generates ribose used as the base in DNA and RNA?
Pentose phosphate pathway
What is the goal of the TCA/Kreb’s cycle?
Makes NADH + H+
Pyruvate enters the TCA/Kreb’s cycle by bindinig to Oxaloacetate making a 6-carbon citrate. Through the cycle it losses 3 carbons to regenerate oxaloacetate, where do they go?
Removed as CO2
Under anaerobic conditions, what happens to the TCA/Creb’s cycle?
It operates as a branched pathway
In EMP pathway of glycolysis is involved with net gain of
2 ATP+ 2NADH
If a baceria is growing on acetate only, what happens to the TCA/Kreb’s cycle?
The glyoxylate shunt is utilized
Pentose Phosphate pathway involves
generation of 5-C sugars as Ribose
Intermediates in TCA cycles are involved in
generation of amino acids as arginine, proline
generation of ATP
Generation of NADH
All the statements are true
All the statements are true
Why must a bacteria ferment if it can’t utilize oxygen?
To regenerate NAD+
Which of the following is NOT true about Electron transport chain
It consist of just one protein ATP Synthase
Oxidative phosphorylation is same as substrate level phosphorylation ?
false
Transport of electrons down the electron transport chain generates.
proton motive force
What molecules generated during glycolysis and the Kreb’s cycle deliver electrons to the electron transport chain?
NADH + H+
Utilization of the PMF to create energy is called what?
Oxidative phosphorylation
In electron transport chain CO2 can be used as terminal electron acceptor?
False
Which of the following components is a membrane-embedded motor with channels connecting the inside and outside of a bacterial cell?
F0-F1 ATP synthase
During glycolysis, ATP is made by what process?
substrate-level phosphorylation
A high level of NADH would have which effect on respiration
Decrease TCA cycle, induce fermentation
Catabolic processes make molecules while anabolic processes break molecules down.
False
In the presence of oxygen, anaerobic organisms utilize which:
Glyoxylate shunt
In the presence of oxygen, anaerobic organisms utilize which:
Glyoxylate shunt
In the presence of oxygen, aerobic organisms utilize the TCA cycle for what purpose?
make NADH+H
We discussed several pathways of glucose metabolism, which is a pathway of fermentation?
Phosphoketolase pathway
Which is not a possible end product of fermentation?
Lactate Ethanol Butanol Formic Acid All are end products
All are end products of fermentation
Which of the following cannot use oxygen for respiration?
Anaerobe
We discussed several pathways of glucose metabolism, which involves the transaldolase and transketolase catalyzed movement of carbons between 5-carbon sugars?
Pentose Phosphate pathway
Entner-Doudoroff pathway
Phosphoketolase pathway
EMP pathway
Pentose Phosphate pathway
In the presence of oxygen, aerobic organisms utilize the TCA cycle for what purpose?
make NADH+ H+
We discussed several pathways of glucose metabolism, which generates the least amount of ATP (not involving TCA or ETC)
Entner-Doudonoff pathway
Which enzyme phosphorylates glucose in the EMP pathway?
Hexokinase
Substrate level phosphorylation involves synthesis of ATP and inorganic phosphate (Pi)
False
Which of the following does not happen in the Embden-Meyerhof-parnas pathway of glycolysis:
generation of PMF
The F0-F1 ATPase can utilize what differential to generate ATP under alkaline environmental conditions?
Glucose
The F0-F1 ATPase can utilize what differential to generate ATP under alkaline environmental conditions?
Glucose
Which enzyme catalyzes the removal of phosphate from PEP yielding the ultimate end product of glycolysis in EMP pathway?
Pyruvate kinase
What unique sugar is generated by the EMP pathway?
KDPG
What is the goal of fermentation?
make NAD+
why is fructose-6-p phosphorylated again using more energy:
when split into glyceraldehyde and dihydroxyacetone, Both products will be phosphorylated
What is the goal of glycolysis?
make pyruvate
Which of the following is false regarding the electron transport chain (ETC) in bacteria :
the ETC proteins pump protons out of the cell
.Lactose is a disaccharide of glucose and galactose, upon cleavage by beta-galactosidase both sugars are phosphorylated. The glucose-6-p is processed through the EMP pathway, what is the next step for the galactose-1-p:
It is epimerized to glucose (while bound to the carrier UDP)
.Lactose is a disaccharide of glucose and galactose, upon cleavage by beta-galactosidase both sugars are phosphorylated. The glucose-6-p is processed through the EMP pathway, what is the next step for the galactose-1-p:
It is epimerized to glucose (while bound to the carrier UDP)
The F0-F1 ATPase can utilize what differential to generate ATP under neutral pH environmental conditions?
Proton
Which of the following is FALSE about the F0-F1 ATPase?
F0-F1 ATPase requires active energy input to generate ATP
Which of the following can use oxygen for respiration?
Aerobe and Facultative anaerobe
Which enzyme rephosphorylates fructose 6-phosphate in the EMP pathway?
Phosphofructokinase
