Exam 2 Structural/Membrane 10/3 Flashcards

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1
Q

Membrane proteins?

A

Peripheral

Integral
- can move laterally
- may exist as micro domains

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2
Q

How do organisms take up nutrients?

A

microbes only take in dissolved etc

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3
Q

Active Transport

A

energy dependent process
move molecules against gradient
concentrated molecules inside cell
involves specific carrier proteins (permeases)

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4
Q

3 types of Active transport

A

Primary
secondary
group translocation

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5
Q

ABC transporters

A
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6
Q

secondary active transport

A
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7
Q

The plasma membrane

A
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8
Q

group translocation phosphotransferase System (PTS)

A

PEP-Sugar (outside) —> putivate + sugar-phosphate (inside)

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9
Q

Bacterial PTS Transport

A

Schematic

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10
Q

Iron uptake special case of active transport

A

microorganisms require iron
ferric iron is very insolvable so uptake is difficult
microorganisms secrete siderophores to aid uptake

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11
Q

The plasma membrane

A

PM can be used to capture energy

embedded electron transport chains help create proton motive force (PMF)
used for respiration/photosynthesis
used to derive energy for motion

PM can hold sensory systems

proteins in the PM can be used to detect environment changes
the cell can use the detected changes to alter gene expression to respond

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12
Q

Siderophores

A

molecule secreted outside of cell

co translational secretion
- in cognition if signal peptide
rest of protein production
messenger rna and slightly synthesize peptide and rhybosomes are produced this way

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13
Q

Some secretion systems of gram negative bacteria

A

Components may have needle like structure to inject or secrete

Schematic

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14
Q

Bacterial Cell Wall

A

Peptidoglycan (murein)

rigid structure that lies just outside the cell membrane

two types based in Gram stain
- gram positive
- gram negative

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15
Q

Cell wall functions

A

maintains shape
helps protect cell from osmotic lysis
protect from toxic materials
may contribute to pathogenicity

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16
Q

Peptidoglycan structure if cell wall

A

mesh like polymer of identical subunits forming long strands

two alternating sugars
- NAG
- NAM

17
Q

The cell wall rigidness

A

Amino acids vary

Cross-linking varies

(strings that stick out can allow formation of peptide bonds)

18
Q

The cell wall D forms

A

D form amino acids associated with NAM in peptidoglycan

D forms are stereoisomers (mirror images) of the L forms normally found in biological proteins

19
Q

lysozyme secretions

A

Look at schematic

20
Q

How can bacteria resist antibiotics?

A

Question asked by professor, might be on exam