Exam 2: Hepatitis Flashcards
Define Hepatitis
- Non- specific inflammation of the hepatocytes, liver cells
- etiology can be single or multifactorial
the pathophysiology of hepatitis
- inflammation triggers fibrogenesis
-hepatic stellate cells are activated, which cause fibrosis - fibrosis can lead to cirrhosis
what does inflammation in the liver cells activate?
it activates stellate cells and these cells make fibrosis
can cirrhotic be reversed?
no, once cirrhotic, it cannot be reversed. there is no cure.
-early stages of cirrhosis, may be reversed with anti-fibrotic agents
what can cause hepatitis? (5)
-heavy alcohol use
-toxins
-some medications
-certain medical conditions
-virus
the common hepatitis viruses in the US
-hepatitis A virus
-hepatitis B virus
-hepatitis C virus
what is the portal triads composed of?
composed of three major tubes
- hepatic artery
-portal vein
-bile duct
function of the hepatic artery
carry oxygenated blood to the hepatocytes
function of the portal vein
carry blood with nutrients from the small intestines
function of the bile duct
carries bile products away from the hepatocytes, to the larger ducts and gallbladder
Serologic Markers (5)
- IgM
o means “Miserable NOW= acute”
if you are worried for an acute infection, you order this! - IgG
o Means “good to go, Gone= chronic”
Exposed at some point in your life - Either by vaccine or actual infection
- Antibody (Ab) Total
o Just checks immunity
When it comes back + = exposed, or vaccinated.
You have immunity towards the virus! - ALT
o More specific for Liver damage
Important lab to follow and monitor! - Antigen (Ag)
o Looking for something infecting, acute!
Surface (sAg) - Outside of the virus
Envelope (eAg) - Around the virus
Core (cAg) - Inside the virus
what is Nonalcoholic Steatohepatitis (NASH)
- Significant form of chronic liver disease in adults and children
- Presence of hepatic steatosis and inflammation with hepatocyte injury (ballooning) with or without fibrosis
o Fat in liver, with inflammation surrounding it. Liver cells blow up like a balloon, then can progress to fibrosis
o Only diagnosed by biopsy - Seen as early as 8 years of age, most common liver abnormality in children from 2 to 19 yrs
the pathogenesis of NASH
o NASH is hepatocellular injury, inflammation, and fibrosis
The most severe forms of NASH progress to cirrhosis, end stage liver disease, and increased risk for hepatocellular carcinoma
o Insulin resistance
o Change in lipogenesis
o Mitochondrial dysfunction
o Cytokine and adipokine association –liver damage and alterations in repair in the fatty liver
o 2 “hit” model or theory
steatosis can be reversible process
“Hit” 1 is excessive hepatocyte triglyceride accumulation resulting from insulin resistance
“Hit” 2 involves an inflammatory injury to the liver possibly by oxidative stress, associated with lipid peroxidation and cytokines
What is Hepatitis A? And what injuries can you get from it?
o Only an ACUTE illness!!!
o A highly contagious, short-term liver infection, caused by the virus Hep A.
o You can get two type of injuries; hepatocellular injury or Cholestasis
what is hepatocellular injury?
- “Basically, injury to the actual liver cells”
- Diffuse liver necrosis
- Prominent Centrilobular damage
- Increased portal cellularity
- Regional lymph node enlargement
what is cholestasis?
- “causing injury to bile ducts, therefore bile leaks out, gets absorbed in the blood, causing jaundice”
- Jaundice, this is a more severe case of Hep. A
- Hyperbilirubinemia
Impaired Synthetic Function - Prolonged PT
- Decreased albumin
pathophysiology of hepatitis A
Viral replication on the liver cells
After entry into cell (virus), RNA is uncoated and ribosomes bind to form polysomes
* -> viral proteins are then synthesized
* -> genome is copied by polymerase
* -> assembled virus particles are shed into biliary tree and excreted into the feces
incubation period for hepatitis A
28 days
-symptoms last about 4-6 weeks
transmission of hepatitis A
o Fecal-oral
Hep A virus bile excretion with shedding in stool occurring 2 weeks prior to and 1 week after onset of symptoms/illness
pertinent patient hx (hep A)
recent travel outside of US
presentation of Hepatitis A (8)
o Flu-like symptoms
o Anorexia
o Headache
o Abdominal pain
o Myalgia
o Dark urine
o Clay colored stools
o Jaundice (common)
Abnormal Liver Enzymes/Serologic Markers: hepatitis A
o AST/ ALT usually elevated >500 in acute infection
o Serological testing
HAV Ab IgG- chronic marker- + test = immune/cleared
HAV Ab IgM- acute marker- + test = acute
HAV Ab Total- does not differentiate between the two tests
* It is helpful to determine immunity, not active acute infection
* May have acquired immunity
complications of hepatitis A
-extremely rare
-transplantation
preventative measures for hepatitis A
o HAV vaccine!!
Available for adults and children
Given to “risky” population
* Military, travelers, moving to endemic areas, illicit drug users, gays
• Serum immunoglobulin is given to
those exposed (HAV Immunoglobulin)
− Pre-exposure prophylaxis
• < 2 week trip
• < 2 y/o
− Post-exposure prophylaxis
• Up to 2 weeks post exposure
• Household, sexual contact, daycare, food
handlers etc.
what is hepatitis B
o Liver infection caused by Hep. B virus
o Member of the hepadnavirus family
o Acute and chronic infection
Some people are sick for a few weeks, others the disease progresses to a serious lifelong illness, chronic HBV.
30% acute, 15% chronic
* 95% clear the infection and develop a lifelong immunity
o **rmbr it is not curable, you can stop the activity, but since it is replicated in the nucleus you cannot get rid of the virus’ DNA
pathophysiology of hepatitis B
Once the body is exposed to HBV, a cell-mediated immune response is triggered
Cytotoxic T cells and natural killer cells are sent to the virus and release inflammatory cytokines
Liver cells are attacked and are infiltrated by HBV
* Ground glass appearance
Virus is constantly being shed into the blood
Replication TAKES PLACE IN NUCLEUS OF THE CELL. “DEEP IN THE CELL” THEREFORE THERE IS NO CURE!
incubation period for hepatitis B
30-60 days
-can survive 7 days outside of the body and still cause infection
transmission of hepatitis B
o Mother to child
20% transmission rate, if HBs Ag positive
90% transmission rate, if HBe Ag positive
o Blood to blood
Mucosal contact with infected blood or body fluid
* Hemodialysis patients are always at risk
Can be transmitted sexually, if mucosal gets torn
* Sometimes semen or saliva has blood
* Having sex on your menstrual cycle
* Perinatal infection is common
pertinent patient hx (risk factors) for hepatitis B
o Are they getting HD
o Have they had transfusion, outside of US
o Sexual encounters
o Someone at home who has it
o Are they IV drug users
o What kind of work do they do
what are three initial testing for HBV
-HBsAg (surface antigen): it is the marker of active disease–first detectable viral antigen
-HBcAb Total (core total): the core protein is part of the nucleus, it will remain positive for life, if they have been exposed or had it
-HBsAb (surface antibody): marker of immunity–it will be (+) if you have had vaccine