Exam 2 eek

Question 1

What would indicate that a signal sequence was necessary for ER translocation?

Answer 1

Deleting it from an ER protein and seeing the protein not localize to he ER

Question 2

What would indicate that a signal sequence was sufficient for ER translocation?

Answer 2

Putting it on a cytosolic protein and seeing it localize to the ER

Question 3

What is N-linked glycosylation in protein folding?

Answer 3

Initial (co-translational) transfer of the precursor chain to the protein through OST

Question 4

When does more modification of the N-glycosylation occur?

Answer 4

Prior to the exit of the protein from the ER to the Golgi

Question 5

What are the successive cycles of trimming of terminal glucose of N-glycosylation?

Answer 5

-They are associated with proper protein folding in ER.
-The final glucose is only removed after successful folding

Question 6

What binds to a monoglucosylated protein ?

Answer 6

Calnexin, a protein chaperone